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Harnessing Intronic microRNA Structures to Improve Tolerance and Expression of shRNAs in Animal Cells

Exogenous RNA polymerase III (pol III) promoters are commonly used to express short hairpin RNA (shRNA). Previous studies have indicated that expression of shRNAs using standard pol III promoters can cause toxicity in vivo due to saturation of the native miRNA pathway. A potential way of mitigating...

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Detalles Bibliográficos
Autores principales:	Challagulla, Arjun, Tizard, Mark L., Doran, Timothy J., Cahill, David M., Jenkins, Kristie A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8879667/ https://www.ncbi.nlm.nih.gov/pubmed/35200534 http://dx.doi.org/10.3390/mps5010018

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