Cargando…
EPA-Enriched Phospholipids Alleviate Renal Interstitial Fibrosis in Spontaneously Hypertensive Rats by Regulating TGF-β Signaling Pathways
Hypertensive nephropathy is a chronic kidney disease caused by hypertension. Eicosapentaenoic acid (EPA) has been reported to possess an antihypertensive effect, and our previous study suggested that EPA-enriched phospholipid (EPA-PL) had more significant bioactivities compared with traditional EPA....
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8879699/ https://www.ncbi.nlm.nih.gov/pubmed/35200681 http://dx.doi.org/10.3390/md20020152 |
_version_ | 1784658956161908736 |
---|---|
author | Shi, Hao-Hao Zhang, Ling-Yu Chen, Li-Pin Yang, Jin-Yue Wang, Cheng-Cheng Xue, Chang-Hu Wang, Yu-Ming Zhang, Tian-Tian |
author_facet | Shi, Hao-Hao Zhang, Ling-Yu Chen, Li-Pin Yang, Jin-Yue Wang, Cheng-Cheng Xue, Chang-Hu Wang, Yu-Ming Zhang, Tian-Tian |
author_sort | Shi, Hao-Hao |
collection | PubMed |
description | Hypertensive nephropathy is a chronic kidney disease caused by hypertension. Eicosapentaenoic acid (EPA) has been reported to possess an antihypertensive effect, and our previous study suggested that EPA-enriched phospholipid (EPA-PL) had more significant bioactivities compared with traditional EPA. However, the effect of dietary EPA-PL on hypertensive nephropathy has not been studied. The current study was designed to examine the protection of EPA-PL against kidney damage in spontaneously hypertensive rats (SHRs). Treatment with EPA-PL for three weeks significantly reduced blood pressure through regulating the renin–angiotensin system in SHRs. Moreover, dietary EPA-PL distinctly alleviated kidney dysfunction in SHRs, evidenced by reduced plasma creatinine, blood urea nitrogen, and 24 h proteinuria. Histology results revealed that treatment of SHRs with EPA-PL alleviated renal injury and reduced tubulointerstitial fibrosis. Further mechanistic studies indicated that dietary EPA-PL remarkably inhibited the activation of TGF-β and Smad 3, elevated the phosphorylation level of PI3K/AKT, suppressed the activation of NF-κB, reduced the expression of pro-inflammatory cytokines, including IL-1β and IL-6, and repressed the oxidative stress and the mitochondria-mediated apoptotic signaling pathway in the kidney. These results indicate that EPA-PL has potential value in the prevention and alleviation of hypertensive nephropathy. |
format | Online Article Text |
id | pubmed-8879699 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88796992022-02-26 EPA-Enriched Phospholipids Alleviate Renal Interstitial Fibrosis in Spontaneously Hypertensive Rats by Regulating TGF-β Signaling Pathways Shi, Hao-Hao Zhang, Ling-Yu Chen, Li-Pin Yang, Jin-Yue Wang, Cheng-Cheng Xue, Chang-Hu Wang, Yu-Ming Zhang, Tian-Tian Mar Drugs Article Hypertensive nephropathy is a chronic kidney disease caused by hypertension. Eicosapentaenoic acid (EPA) has been reported to possess an antihypertensive effect, and our previous study suggested that EPA-enriched phospholipid (EPA-PL) had more significant bioactivities compared with traditional EPA. However, the effect of dietary EPA-PL on hypertensive nephropathy has not been studied. The current study was designed to examine the protection of EPA-PL against kidney damage in spontaneously hypertensive rats (SHRs). Treatment with EPA-PL for three weeks significantly reduced blood pressure through regulating the renin–angiotensin system in SHRs. Moreover, dietary EPA-PL distinctly alleviated kidney dysfunction in SHRs, evidenced by reduced plasma creatinine, blood urea nitrogen, and 24 h proteinuria. Histology results revealed that treatment of SHRs with EPA-PL alleviated renal injury and reduced tubulointerstitial fibrosis. Further mechanistic studies indicated that dietary EPA-PL remarkably inhibited the activation of TGF-β and Smad 3, elevated the phosphorylation level of PI3K/AKT, suppressed the activation of NF-κB, reduced the expression of pro-inflammatory cytokines, including IL-1β and IL-6, and repressed the oxidative stress and the mitochondria-mediated apoptotic signaling pathway in the kidney. These results indicate that EPA-PL has potential value in the prevention and alleviation of hypertensive nephropathy. MDPI 2022-02-19 /pmc/articles/PMC8879699/ /pubmed/35200681 http://dx.doi.org/10.3390/md20020152 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Shi, Hao-Hao Zhang, Ling-Yu Chen, Li-Pin Yang, Jin-Yue Wang, Cheng-Cheng Xue, Chang-Hu Wang, Yu-Ming Zhang, Tian-Tian EPA-Enriched Phospholipids Alleviate Renal Interstitial Fibrosis in Spontaneously Hypertensive Rats by Regulating TGF-β Signaling Pathways |
title | EPA-Enriched Phospholipids Alleviate Renal Interstitial Fibrosis in Spontaneously Hypertensive Rats by Regulating TGF-β Signaling Pathways |
title_full | EPA-Enriched Phospholipids Alleviate Renal Interstitial Fibrosis in Spontaneously Hypertensive Rats by Regulating TGF-β Signaling Pathways |
title_fullStr | EPA-Enriched Phospholipids Alleviate Renal Interstitial Fibrosis in Spontaneously Hypertensive Rats by Regulating TGF-β Signaling Pathways |
title_full_unstemmed | EPA-Enriched Phospholipids Alleviate Renal Interstitial Fibrosis in Spontaneously Hypertensive Rats by Regulating TGF-β Signaling Pathways |
title_short | EPA-Enriched Phospholipids Alleviate Renal Interstitial Fibrosis in Spontaneously Hypertensive Rats by Regulating TGF-β Signaling Pathways |
title_sort | epa-enriched phospholipids alleviate renal interstitial fibrosis in spontaneously hypertensive rats by regulating tgf-β signaling pathways |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8879699/ https://www.ncbi.nlm.nih.gov/pubmed/35200681 http://dx.doi.org/10.3390/md20020152 |
work_keys_str_mv | AT shihaohao epaenrichedphospholipidsalleviaterenalinterstitialfibrosisinspontaneouslyhypertensiveratsbyregulatingtgfbsignalingpathways AT zhanglingyu epaenrichedphospholipidsalleviaterenalinterstitialfibrosisinspontaneouslyhypertensiveratsbyregulatingtgfbsignalingpathways AT chenlipin epaenrichedphospholipidsalleviaterenalinterstitialfibrosisinspontaneouslyhypertensiveratsbyregulatingtgfbsignalingpathways AT yangjinyue epaenrichedphospholipidsalleviaterenalinterstitialfibrosisinspontaneouslyhypertensiveratsbyregulatingtgfbsignalingpathways AT wangchengcheng epaenrichedphospholipidsalleviaterenalinterstitialfibrosisinspontaneouslyhypertensiveratsbyregulatingtgfbsignalingpathways AT xuechanghu epaenrichedphospholipidsalleviaterenalinterstitialfibrosisinspontaneouslyhypertensiveratsbyregulatingtgfbsignalingpathways AT wangyuming epaenrichedphospholipidsalleviaterenalinterstitialfibrosisinspontaneouslyhypertensiveratsbyregulatingtgfbsignalingpathways AT zhangtiantian epaenrichedphospholipidsalleviaterenalinterstitialfibrosisinspontaneouslyhypertensiveratsbyregulatingtgfbsignalingpathways |