Cargando…

EPA-Enriched Phospholipids Alleviate Renal Interstitial Fibrosis in Spontaneously Hypertensive Rats by Regulating TGF-β Signaling Pathways

Hypertensive nephropathy is a chronic kidney disease caused by hypertension. Eicosapentaenoic acid (EPA) has been reported to possess an antihypertensive effect, and our previous study suggested that EPA-enriched phospholipid (EPA-PL) had more significant bioactivities compared with traditional EPA....

Descripción completa

Detalles Bibliográficos
Autores principales: Shi, Hao-Hao, Zhang, Ling-Yu, Chen, Li-Pin, Yang, Jin-Yue, Wang, Cheng-Cheng, Xue, Chang-Hu, Wang, Yu-Ming, Zhang, Tian-Tian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8879699/
https://www.ncbi.nlm.nih.gov/pubmed/35200681
http://dx.doi.org/10.3390/md20020152
_version_ 1784658956161908736
author Shi, Hao-Hao
Zhang, Ling-Yu
Chen, Li-Pin
Yang, Jin-Yue
Wang, Cheng-Cheng
Xue, Chang-Hu
Wang, Yu-Ming
Zhang, Tian-Tian
author_facet Shi, Hao-Hao
Zhang, Ling-Yu
Chen, Li-Pin
Yang, Jin-Yue
Wang, Cheng-Cheng
Xue, Chang-Hu
Wang, Yu-Ming
Zhang, Tian-Tian
author_sort Shi, Hao-Hao
collection PubMed
description Hypertensive nephropathy is a chronic kidney disease caused by hypertension. Eicosapentaenoic acid (EPA) has been reported to possess an antihypertensive effect, and our previous study suggested that EPA-enriched phospholipid (EPA-PL) had more significant bioactivities compared with traditional EPA. However, the effect of dietary EPA-PL on hypertensive nephropathy has not been studied. The current study was designed to examine the protection of EPA-PL against kidney damage in spontaneously hypertensive rats (SHRs). Treatment with EPA-PL for three weeks significantly reduced blood pressure through regulating the renin–angiotensin system in SHRs. Moreover, dietary EPA-PL distinctly alleviated kidney dysfunction in SHRs, evidenced by reduced plasma creatinine, blood urea nitrogen, and 24 h proteinuria. Histology results revealed that treatment of SHRs with EPA-PL alleviated renal injury and reduced tubulointerstitial fibrosis. Further mechanistic studies indicated that dietary EPA-PL remarkably inhibited the activation of TGF-β and Smad 3, elevated the phosphorylation level of PI3K/AKT, suppressed the activation of NF-κB, reduced the expression of pro-inflammatory cytokines, including IL-1β and IL-6, and repressed the oxidative stress and the mitochondria-mediated apoptotic signaling pathway in the kidney. These results indicate that EPA-PL has potential value in the prevention and alleviation of hypertensive nephropathy.
format Online
Article
Text
id pubmed-8879699
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-88796992022-02-26 EPA-Enriched Phospholipids Alleviate Renal Interstitial Fibrosis in Spontaneously Hypertensive Rats by Regulating TGF-β Signaling Pathways Shi, Hao-Hao Zhang, Ling-Yu Chen, Li-Pin Yang, Jin-Yue Wang, Cheng-Cheng Xue, Chang-Hu Wang, Yu-Ming Zhang, Tian-Tian Mar Drugs Article Hypertensive nephropathy is a chronic kidney disease caused by hypertension. Eicosapentaenoic acid (EPA) has been reported to possess an antihypertensive effect, and our previous study suggested that EPA-enriched phospholipid (EPA-PL) had more significant bioactivities compared with traditional EPA. However, the effect of dietary EPA-PL on hypertensive nephropathy has not been studied. The current study was designed to examine the protection of EPA-PL against kidney damage in spontaneously hypertensive rats (SHRs). Treatment with EPA-PL for three weeks significantly reduced blood pressure through regulating the renin–angiotensin system in SHRs. Moreover, dietary EPA-PL distinctly alleviated kidney dysfunction in SHRs, evidenced by reduced plasma creatinine, blood urea nitrogen, and 24 h proteinuria. Histology results revealed that treatment of SHRs with EPA-PL alleviated renal injury and reduced tubulointerstitial fibrosis. Further mechanistic studies indicated that dietary EPA-PL remarkably inhibited the activation of TGF-β and Smad 3, elevated the phosphorylation level of PI3K/AKT, suppressed the activation of NF-κB, reduced the expression of pro-inflammatory cytokines, including IL-1β and IL-6, and repressed the oxidative stress and the mitochondria-mediated apoptotic signaling pathway in the kidney. These results indicate that EPA-PL has potential value in the prevention and alleviation of hypertensive nephropathy. MDPI 2022-02-19 /pmc/articles/PMC8879699/ /pubmed/35200681 http://dx.doi.org/10.3390/md20020152 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shi, Hao-Hao
Zhang, Ling-Yu
Chen, Li-Pin
Yang, Jin-Yue
Wang, Cheng-Cheng
Xue, Chang-Hu
Wang, Yu-Ming
Zhang, Tian-Tian
EPA-Enriched Phospholipids Alleviate Renal Interstitial Fibrosis in Spontaneously Hypertensive Rats by Regulating TGF-β Signaling Pathways
title EPA-Enriched Phospholipids Alleviate Renal Interstitial Fibrosis in Spontaneously Hypertensive Rats by Regulating TGF-β Signaling Pathways
title_full EPA-Enriched Phospholipids Alleviate Renal Interstitial Fibrosis in Spontaneously Hypertensive Rats by Regulating TGF-β Signaling Pathways
title_fullStr EPA-Enriched Phospholipids Alleviate Renal Interstitial Fibrosis in Spontaneously Hypertensive Rats by Regulating TGF-β Signaling Pathways
title_full_unstemmed EPA-Enriched Phospholipids Alleviate Renal Interstitial Fibrosis in Spontaneously Hypertensive Rats by Regulating TGF-β Signaling Pathways
title_short EPA-Enriched Phospholipids Alleviate Renal Interstitial Fibrosis in Spontaneously Hypertensive Rats by Regulating TGF-β Signaling Pathways
title_sort epa-enriched phospholipids alleviate renal interstitial fibrosis in spontaneously hypertensive rats by regulating tgf-β signaling pathways
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8879699/
https://www.ncbi.nlm.nih.gov/pubmed/35200681
http://dx.doi.org/10.3390/md20020152
work_keys_str_mv AT shihaohao epaenrichedphospholipidsalleviaterenalinterstitialfibrosisinspontaneouslyhypertensiveratsbyregulatingtgfbsignalingpathways
AT zhanglingyu epaenrichedphospholipidsalleviaterenalinterstitialfibrosisinspontaneouslyhypertensiveratsbyregulatingtgfbsignalingpathways
AT chenlipin epaenrichedphospholipidsalleviaterenalinterstitialfibrosisinspontaneouslyhypertensiveratsbyregulatingtgfbsignalingpathways
AT yangjinyue epaenrichedphospholipidsalleviaterenalinterstitialfibrosisinspontaneouslyhypertensiveratsbyregulatingtgfbsignalingpathways
AT wangchengcheng epaenrichedphospholipidsalleviaterenalinterstitialfibrosisinspontaneouslyhypertensiveratsbyregulatingtgfbsignalingpathways
AT xuechanghu epaenrichedphospholipidsalleviaterenalinterstitialfibrosisinspontaneouslyhypertensiveratsbyregulatingtgfbsignalingpathways
AT wangyuming epaenrichedphospholipidsalleviaterenalinterstitialfibrosisinspontaneouslyhypertensiveratsbyregulatingtgfbsignalingpathways
AT zhangtiantian epaenrichedphospholipidsalleviaterenalinterstitialfibrosisinspontaneouslyhypertensiveratsbyregulatingtgfbsignalingpathways