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Presence of Helicobacter Species in Gastric Mucosa of Human Patients and Outcome of Helicobacter Eradication Treatment

The genus Helicobacter is composed of bacteria that colonize both the human and animal gastrointestinal tract. Helicobacter pylori infects half of the world’s population, causing various disorders, such as gastritis, duodenitis and gastric cancer. Additionally, non-Helicobacter pylori Helicobacter s...

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Autores principales: Matos, Rita, Taillieu, Emily, De Bruyckere, Sofie, De Witte, Chloë, Rêma, Alexandra, Santos-Sousa, Hugo, Nogueiro, Jorge, Reis, Celso A., Carneiro, Fátima, Haesebrouck, Freddy, Amorim, Irina, Gärtner, Fátima
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8879780/
https://www.ncbi.nlm.nih.gov/pubmed/35207669
http://dx.doi.org/10.3390/jpm12020181
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author Matos, Rita
Taillieu, Emily
De Bruyckere, Sofie
De Witte, Chloë
Rêma, Alexandra
Santos-Sousa, Hugo
Nogueiro, Jorge
Reis, Celso A.
Carneiro, Fátima
Haesebrouck, Freddy
Amorim, Irina
Gärtner, Fátima
author_facet Matos, Rita
Taillieu, Emily
De Bruyckere, Sofie
De Witte, Chloë
Rêma, Alexandra
Santos-Sousa, Hugo
Nogueiro, Jorge
Reis, Celso A.
Carneiro, Fátima
Haesebrouck, Freddy
Amorim, Irina
Gärtner, Fátima
author_sort Matos, Rita
collection PubMed
description The genus Helicobacter is composed of bacteria that colonize both the human and animal gastrointestinal tract. Helicobacter pylori infects half of the world’s population, causing various disorders, such as gastritis, duodenitis and gastric cancer. Additionally, non-Helicobacter pylori Helicobacter species (NHPH) are commonly found in the stomach of pigs, dogs and cats. Most of these species have zoonotic potential and prevalence rates of 0.2–6.0%, and have been described in human patients suffering from gastric disorders undergoing a gastric biopsy. The aim of this study was to determine the occurrence of Helicobacter spp. in the stomach of patients with gastric cancer (n = 17) and obese (n = 63) patients. Furthermore, the outcome of the Helicobacter eradication treatment and the current infection status was evaluated. Overall, based on the genus-specific PCR followed by sequencing, DNA from Helicobacter spp. was detected in 46.3% of the patients, including single infections with H. pylori in 43.8% of the patients and mixed infections with H. pylori and canine- or feline-associated H. felis in 2.5%. About 32.5% of the patients had been subjected to previous Helicobacter eradication therapy and the triple standard therapy was the most frequent scheme (42.3%). In 48.0% of the patients who received eradication treatment, bacteria were still detected, including one mixed infection. In 23.1% of the patients who reported that a subsequent test had been performed to confirm the elimination of the bacteria, Helicobacter were still detected. In conclusion, although in a smaller percentage, NHPH may also be present in the human stomach. Thus, specific NHPH screening should be included in the diagnostic routine. The continued presence of H. pylori in the stomach of patients recently subjected to eradication schemes raises questions about the efficacy of the current Helicobacter treatments.
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spelling pubmed-88797802022-02-26 Presence of Helicobacter Species in Gastric Mucosa of Human Patients and Outcome of Helicobacter Eradication Treatment Matos, Rita Taillieu, Emily De Bruyckere, Sofie De Witte, Chloë Rêma, Alexandra Santos-Sousa, Hugo Nogueiro, Jorge Reis, Celso A. Carneiro, Fátima Haesebrouck, Freddy Amorim, Irina Gärtner, Fátima J Pers Med Article The genus Helicobacter is composed of bacteria that colonize both the human and animal gastrointestinal tract. Helicobacter pylori infects half of the world’s population, causing various disorders, such as gastritis, duodenitis and gastric cancer. Additionally, non-Helicobacter pylori Helicobacter species (NHPH) are commonly found in the stomach of pigs, dogs and cats. Most of these species have zoonotic potential and prevalence rates of 0.2–6.0%, and have been described in human patients suffering from gastric disorders undergoing a gastric biopsy. The aim of this study was to determine the occurrence of Helicobacter spp. in the stomach of patients with gastric cancer (n = 17) and obese (n = 63) patients. Furthermore, the outcome of the Helicobacter eradication treatment and the current infection status was evaluated. Overall, based on the genus-specific PCR followed by sequencing, DNA from Helicobacter spp. was detected in 46.3% of the patients, including single infections with H. pylori in 43.8% of the patients and mixed infections with H. pylori and canine- or feline-associated H. felis in 2.5%. About 32.5% of the patients had been subjected to previous Helicobacter eradication therapy and the triple standard therapy was the most frequent scheme (42.3%). In 48.0% of the patients who received eradication treatment, bacteria were still detected, including one mixed infection. In 23.1% of the patients who reported that a subsequent test had been performed to confirm the elimination of the bacteria, Helicobacter were still detected. In conclusion, although in a smaller percentage, NHPH may also be present in the human stomach. Thus, specific NHPH screening should be included in the diagnostic routine. The continued presence of H. pylori in the stomach of patients recently subjected to eradication schemes raises questions about the efficacy of the current Helicobacter treatments. MDPI 2022-01-29 /pmc/articles/PMC8879780/ /pubmed/35207669 http://dx.doi.org/10.3390/jpm12020181 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Matos, Rita
Taillieu, Emily
De Bruyckere, Sofie
De Witte, Chloë
Rêma, Alexandra
Santos-Sousa, Hugo
Nogueiro, Jorge
Reis, Celso A.
Carneiro, Fátima
Haesebrouck, Freddy
Amorim, Irina
Gärtner, Fátima
Presence of Helicobacter Species in Gastric Mucosa of Human Patients and Outcome of Helicobacter Eradication Treatment
title Presence of Helicobacter Species in Gastric Mucosa of Human Patients and Outcome of Helicobacter Eradication Treatment
title_full Presence of Helicobacter Species in Gastric Mucosa of Human Patients and Outcome of Helicobacter Eradication Treatment
title_fullStr Presence of Helicobacter Species in Gastric Mucosa of Human Patients and Outcome of Helicobacter Eradication Treatment
title_full_unstemmed Presence of Helicobacter Species in Gastric Mucosa of Human Patients and Outcome of Helicobacter Eradication Treatment
title_short Presence of Helicobacter Species in Gastric Mucosa of Human Patients and Outcome of Helicobacter Eradication Treatment
title_sort presence of helicobacter species in gastric mucosa of human patients and outcome of helicobacter eradication treatment
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8879780/
https://www.ncbi.nlm.nih.gov/pubmed/35207669
http://dx.doi.org/10.3390/jpm12020181
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