Cargando…

Chikungunya Virus’ High Genomic Plasticity Enables Rapid Adaptation to Restrictive A549 Cells

Chikungunya virus (CHIKV) is an emerging arthropod-borne virus that has spread globally during the last two decades. The virus is mainly transmitted by Aedes aegypti and Aedes albopictus mosquitos and is thus capable of replicating in both human and mosquito cells. CHIKV has a broad tropism in vivo,...

Descripción completa

Detalles Bibliográficos
Autores principales: De Caluwé, Lien, Heyndrickx, Leo, Coppens, Sandra, Vereecken, Katleen, Quiñones-Mateu, Miguel E., Merits, Andres, Ariën, Kevin K., Bartholomeeusen, Koen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8879786/
https://www.ncbi.nlm.nih.gov/pubmed/35215875
http://dx.doi.org/10.3390/v14020282
_version_ 1784658990161985536
author De Caluwé, Lien
Heyndrickx, Leo
Coppens, Sandra
Vereecken, Katleen
Quiñones-Mateu, Miguel E.
Merits, Andres
Ariën, Kevin K.
Bartholomeeusen, Koen
author_facet De Caluwé, Lien
Heyndrickx, Leo
Coppens, Sandra
Vereecken, Katleen
Quiñones-Mateu, Miguel E.
Merits, Andres
Ariën, Kevin K.
Bartholomeeusen, Koen
author_sort De Caluwé, Lien
collection PubMed
description Chikungunya virus (CHIKV) is an emerging arthropod-borne virus that has spread globally during the last two decades. The virus is mainly transmitted by Aedes aegypti and Aedes albopictus mosquitos and is thus capable of replicating in both human and mosquito cells. CHIKV has a broad tropism in vivo, capable of replicating in various tissues and cell types but largely excluding blood cells. This was reflected in vitro by a broad array of adherent cell lines supporting CHIKV infection. One marked exception to this general rule is the resistance of the lung cancer-derived A549 cell line to CHIKV infection. We verified that A549 cells were restrictive to infection by multiple alphaviruses while being completely permissive to flavivirus infection. The adaptive growth of a primary CHIKV strain through multiple passages allowed the emergence of a CHIKV strain that productively infected A549 cells while causing overt cytopathic effects and without a fitness cost for replication in otherwise CHIKV-susceptible cells. Whole genome sequencing of polyclonal and monoclonal preparations of the adapted virus showed that a limited number of mutations consistently emerged in both structural (2 mutations in E2) and non-structural proteins (1 mutation in nsP1 and 1 mutation in nsP2). The introduction of the adaptive mutations, individually or in combinations, into a wild-type molecular clone of CHIKV allowed us to determine the relative contributions of the mutations to the new phenotype. We found that the mutations in the E2 envelope protein and non-structural proteins contributed significantly to the acquired phenotype. The nsP mutations were introduced in a split-genome trans-replicase assay to monitor their effect on viral genome replication efficiency. Interestingly, neither mutation supported increased viral genomic replication in either Vero or A549 cells.
format Online
Article
Text
id pubmed-8879786
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-88797862022-02-26 Chikungunya Virus’ High Genomic Plasticity Enables Rapid Adaptation to Restrictive A549 Cells De Caluwé, Lien Heyndrickx, Leo Coppens, Sandra Vereecken, Katleen Quiñones-Mateu, Miguel E. Merits, Andres Ariën, Kevin K. Bartholomeeusen, Koen Viruses Article Chikungunya virus (CHIKV) is an emerging arthropod-borne virus that has spread globally during the last two decades. The virus is mainly transmitted by Aedes aegypti and Aedes albopictus mosquitos and is thus capable of replicating in both human and mosquito cells. CHIKV has a broad tropism in vivo, capable of replicating in various tissues and cell types but largely excluding blood cells. This was reflected in vitro by a broad array of adherent cell lines supporting CHIKV infection. One marked exception to this general rule is the resistance of the lung cancer-derived A549 cell line to CHIKV infection. We verified that A549 cells were restrictive to infection by multiple alphaviruses while being completely permissive to flavivirus infection. The adaptive growth of a primary CHIKV strain through multiple passages allowed the emergence of a CHIKV strain that productively infected A549 cells while causing overt cytopathic effects and without a fitness cost for replication in otherwise CHIKV-susceptible cells. Whole genome sequencing of polyclonal and monoclonal preparations of the adapted virus showed that a limited number of mutations consistently emerged in both structural (2 mutations in E2) and non-structural proteins (1 mutation in nsP1 and 1 mutation in nsP2). The introduction of the adaptive mutations, individually or in combinations, into a wild-type molecular clone of CHIKV allowed us to determine the relative contributions of the mutations to the new phenotype. We found that the mutations in the E2 envelope protein and non-structural proteins contributed significantly to the acquired phenotype. The nsP mutations were introduced in a split-genome trans-replicase assay to monitor their effect on viral genome replication efficiency. Interestingly, neither mutation supported increased viral genomic replication in either Vero or A549 cells. MDPI 2022-01-28 /pmc/articles/PMC8879786/ /pubmed/35215875 http://dx.doi.org/10.3390/v14020282 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
De Caluwé, Lien
Heyndrickx, Leo
Coppens, Sandra
Vereecken, Katleen
Quiñones-Mateu, Miguel E.
Merits, Andres
Ariën, Kevin K.
Bartholomeeusen, Koen
Chikungunya Virus’ High Genomic Plasticity Enables Rapid Adaptation to Restrictive A549 Cells
title Chikungunya Virus’ High Genomic Plasticity Enables Rapid Adaptation to Restrictive A549 Cells
title_full Chikungunya Virus’ High Genomic Plasticity Enables Rapid Adaptation to Restrictive A549 Cells
title_fullStr Chikungunya Virus’ High Genomic Plasticity Enables Rapid Adaptation to Restrictive A549 Cells
title_full_unstemmed Chikungunya Virus’ High Genomic Plasticity Enables Rapid Adaptation to Restrictive A549 Cells
title_short Chikungunya Virus’ High Genomic Plasticity Enables Rapid Adaptation to Restrictive A549 Cells
title_sort chikungunya virus’ high genomic plasticity enables rapid adaptation to restrictive a549 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8879786/
https://www.ncbi.nlm.nih.gov/pubmed/35215875
http://dx.doi.org/10.3390/v14020282
work_keys_str_mv AT decaluwelien chikungunyavirushighgenomicplasticityenablesrapidadaptationtorestrictivea549cells
AT heyndrickxleo chikungunyavirushighgenomicplasticityenablesrapidadaptationtorestrictivea549cells
AT coppenssandra chikungunyavirushighgenomicplasticityenablesrapidadaptationtorestrictivea549cells
AT vereeckenkatleen chikungunyavirushighgenomicplasticityenablesrapidadaptationtorestrictivea549cells
AT quinonesmateumiguele chikungunyavirushighgenomicplasticityenablesrapidadaptationtorestrictivea549cells
AT meritsandres chikungunyavirushighgenomicplasticityenablesrapidadaptationtorestrictivea549cells
AT arienkevink chikungunyavirushighgenomicplasticityenablesrapidadaptationtorestrictivea549cells
AT bartholomeeusenkoen chikungunyavirushighgenomicplasticityenablesrapidadaptationtorestrictivea549cells