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Reliable and Scalable SARS-CoV-2 qPCR Testing at a High Sample Throughput: Lessons Learned from the Belgian Initiative
We present our approach to rapidly establishing a standardized, multi-site, nation-wide COVID-19 screening program in Belgium. Under auspices of a federal government Task Force responsible for upscaling the country’s testing capacity, we were able to set up a national testing initiative with readily...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8879918/ https://www.ncbi.nlm.nih.gov/pubmed/35207446 http://dx.doi.org/10.3390/life12020159 |
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author | Van Vooren, Steven Grayson, James Van Ranst, Marc Dequeker, Elisabeth Laenen, Lies Janssen, Reile Gillet, Laurent Bureau, Fabrice Coppieters, Wouter Devos, Nathalie Hengchen, Benjamin Wattiau, Pierre Méhauden, Sibylle Verlinden, Yvan Van Baelen, Kurt Pattery, Theresa Valentin, Jean-Pierre Janssen, Kris Geraerts, Martine Smeraglia, John Hellemans, Jan Wytynck, Pieter Mestdagh, Pieter Besbrugge, Nienke Höfer, René Nollet, Friedel Vandesompele, Jo De Smet, Pieter Lebon, John Vandewynckele, Emmanuel Verstrepen, Steven Uten, Wouter Capron, Arnaud Malonne, Hugues Poels, Jeroen André, Emmanuel |
author_facet | Van Vooren, Steven Grayson, James Van Ranst, Marc Dequeker, Elisabeth Laenen, Lies Janssen, Reile Gillet, Laurent Bureau, Fabrice Coppieters, Wouter Devos, Nathalie Hengchen, Benjamin Wattiau, Pierre Méhauden, Sibylle Verlinden, Yvan Van Baelen, Kurt Pattery, Theresa Valentin, Jean-Pierre Janssen, Kris Geraerts, Martine Smeraglia, John Hellemans, Jan Wytynck, Pieter Mestdagh, Pieter Besbrugge, Nienke Höfer, René Nollet, Friedel Vandesompele, Jo De Smet, Pieter Lebon, John Vandewynckele, Emmanuel Verstrepen, Steven Uten, Wouter Capron, Arnaud Malonne, Hugues Poels, Jeroen André, Emmanuel |
author_sort | Van Vooren, Steven |
collection | PubMed |
description | We present our approach to rapidly establishing a standardized, multi-site, nation-wide COVID-19 screening program in Belgium. Under auspices of a federal government Task Force responsible for upscaling the country’s testing capacity, we were able to set up a national testing initiative with readily available resources, putting in place a robust, validated, high-throughput, and decentralized qPCR molecular testing platform with embedded proficiency testing. We demonstrate how during an acute scarcity of equipment, kits, reagents, personnel, protective equipment, and sterile plastic supplies, we introduced an approach to rapidly build a reliable, validated, high-volume, high-confidence workflow based on heterogeneous instrumentation and diverse assays, assay components, and protocols. The workflow was set up with continuous quality control monitoring, tied together through a clinical-grade information management platform for automated data analysis, real-time result reporting across different participating sites, qc monitoring, and making result data available to the requesting physician and the patient. In this overview, we address challenges in optimizing high-throughput cross-laboratory workflows with minimal manual intervention through software, instrument and assay validation and standardization, and a process for harmonized result reporting and nation-level infection statistics monitoring across the disparate testing methodologies and workflows, necessitated by a rapid scale-up as a response to the pandemic. |
format | Online Article Text |
id | pubmed-8879918 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88799182022-02-26 Reliable and Scalable SARS-CoV-2 qPCR Testing at a High Sample Throughput: Lessons Learned from the Belgian Initiative Van Vooren, Steven Grayson, James Van Ranst, Marc Dequeker, Elisabeth Laenen, Lies Janssen, Reile Gillet, Laurent Bureau, Fabrice Coppieters, Wouter Devos, Nathalie Hengchen, Benjamin Wattiau, Pierre Méhauden, Sibylle Verlinden, Yvan Van Baelen, Kurt Pattery, Theresa Valentin, Jean-Pierre Janssen, Kris Geraerts, Martine Smeraglia, John Hellemans, Jan Wytynck, Pieter Mestdagh, Pieter Besbrugge, Nienke Höfer, René Nollet, Friedel Vandesompele, Jo De Smet, Pieter Lebon, John Vandewynckele, Emmanuel Verstrepen, Steven Uten, Wouter Capron, Arnaud Malonne, Hugues Poels, Jeroen André, Emmanuel Life (Basel) Article We present our approach to rapidly establishing a standardized, multi-site, nation-wide COVID-19 screening program in Belgium. Under auspices of a federal government Task Force responsible for upscaling the country’s testing capacity, we were able to set up a national testing initiative with readily available resources, putting in place a robust, validated, high-throughput, and decentralized qPCR molecular testing platform with embedded proficiency testing. We demonstrate how during an acute scarcity of equipment, kits, reagents, personnel, protective equipment, and sterile plastic supplies, we introduced an approach to rapidly build a reliable, validated, high-volume, high-confidence workflow based on heterogeneous instrumentation and diverse assays, assay components, and protocols. The workflow was set up with continuous quality control monitoring, tied together through a clinical-grade information management platform for automated data analysis, real-time result reporting across different participating sites, qc monitoring, and making result data available to the requesting physician and the patient. In this overview, we address challenges in optimizing high-throughput cross-laboratory workflows with minimal manual intervention through software, instrument and assay validation and standardization, and a process for harmonized result reporting and nation-level infection statistics monitoring across the disparate testing methodologies and workflows, necessitated by a rapid scale-up as a response to the pandemic. MDPI 2022-01-21 /pmc/articles/PMC8879918/ /pubmed/35207446 http://dx.doi.org/10.3390/life12020159 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Van Vooren, Steven Grayson, James Van Ranst, Marc Dequeker, Elisabeth Laenen, Lies Janssen, Reile Gillet, Laurent Bureau, Fabrice Coppieters, Wouter Devos, Nathalie Hengchen, Benjamin Wattiau, Pierre Méhauden, Sibylle Verlinden, Yvan Van Baelen, Kurt Pattery, Theresa Valentin, Jean-Pierre Janssen, Kris Geraerts, Martine Smeraglia, John Hellemans, Jan Wytynck, Pieter Mestdagh, Pieter Besbrugge, Nienke Höfer, René Nollet, Friedel Vandesompele, Jo De Smet, Pieter Lebon, John Vandewynckele, Emmanuel Verstrepen, Steven Uten, Wouter Capron, Arnaud Malonne, Hugues Poels, Jeroen André, Emmanuel Reliable and Scalable SARS-CoV-2 qPCR Testing at a High Sample Throughput: Lessons Learned from the Belgian Initiative |
title | Reliable and Scalable SARS-CoV-2 qPCR Testing at a High Sample Throughput: Lessons Learned from the Belgian Initiative |
title_full | Reliable and Scalable SARS-CoV-2 qPCR Testing at a High Sample Throughput: Lessons Learned from the Belgian Initiative |
title_fullStr | Reliable and Scalable SARS-CoV-2 qPCR Testing at a High Sample Throughput: Lessons Learned from the Belgian Initiative |
title_full_unstemmed | Reliable and Scalable SARS-CoV-2 qPCR Testing at a High Sample Throughput: Lessons Learned from the Belgian Initiative |
title_short | Reliable and Scalable SARS-CoV-2 qPCR Testing at a High Sample Throughput: Lessons Learned from the Belgian Initiative |
title_sort | reliable and scalable sars-cov-2 qpcr testing at a high sample throughput: lessons learned from the belgian initiative |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8879918/ https://www.ncbi.nlm.nih.gov/pubmed/35207446 http://dx.doi.org/10.3390/life12020159 |
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