Cargando…

Reliable and Scalable SARS-CoV-2 qPCR Testing at a High Sample Throughput: Lessons Learned from the Belgian Initiative

We present our approach to rapidly establishing a standardized, multi-site, nation-wide COVID-19 screening program in Belgium. Under auspices of a federal government Task Force responsible for upscaling the country’s testing capacity, we were able to set up a national testing initiative with readily...

Descripción completa

Detalles Bibliográficos
Autores principales: Van Vooren, Steven, Grayson, James, Van Ranst, Marc, Dequeker, Elisabeth, Laenen, Lies, Janssen, Reile, Gillet, Laurent, Bureau, Fabrice, Coppieters, Wouter, Devos, Nathalie, Hengchen, Benjamin, Wattiau, Pierre, Méhauden, Sibylle, Verlinden, Yvan, Van Baelen, Kurt, Pattery, Theresa, Valentin, Jean-Pierre, Janssen, Kris, Geraerts, Martine, Smeraglia, John, Hellemans, Jan, Wytynck, Pieter, Mestdagh, Pieter, Besbrugge, Nienke, Höfer, René, Nollet, Friedel, Vandesompele, Jo, De Smet, Pieter, Lebon, John, Vandewynckele, Emmanuel, Verstrepen, Steven, Uten, Wouter, Capron, Arnaud, Malonne, Hugues, Poels, Jeroen, André, Emmanuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8879918/
https://www.ncbi.nlm.nih.gov/pubmed/35207446
http://dx.doi.org/10.3390/life12020159
_version_ 1784659034114097152
author Van Vooren, Steven
Grayson, James
Van Ranst, Marc
Dequeker, Elisabeth
Laenen, Lies
Janssen, Reile
Gillet, Laurent
Bureau, Fabrice
Coppieters, Wouter
Devos, Nathalie
Hengchen, Benjamin
Wattiau, Pierre
Méhauden, Sibylle
Verlinden, Yvan
Van Baelen, Kurt
Pattery, Theresa
Valentin, Jean-Pierre
Janssen, Kris
Geraerts, Martine
Smeraglia, John
Hellemans, Jan
Wytynck, Pieter
Mestdagh, Pieter
Besbrugge, Nienke
Höfer, René
Nollet, Friedel
Vandesompele, Jo
De Smet, Pieter
Lebon, John
Vandewynckele, Emmanuel
Verstrepen, Steven
Uten, Wouter
Capron, Arnaud
Malonne, Hugues
Poels, Jeroen
André, Emmanuel
author_facet Van Vooren, Steven
Grayson, James
Van Ranst, Marc
Dequeker, Elisabeth
Laenen, Lies
Janssen, Reile
Gillet, Laurent
Bureau, Fabrice
Coppieters, Wouter
Devos, Nathalie
Hengchen, Benjamin
Wattiau, Pierre
Méhauden, Sibylle
Verlinden, Yvan
Van Baelen, Kurt
Pattery, Theresa
Valentin, Jean-Pierre
Janssen, Kris
Geraerts, Martine
Smeraglia, John
Hellemans, Jan
Wytynck, Pieter
Mestdagh, Pieter
Besbrugge, Nienke
Höfer, René
Nollet, Friedel
Vandesompele, Jo
De Smet, Pieter
Lebon, John
Vandewynckele, Emmanuel
Verstrepen, Steven
Uten, Wouter
Capron, Arnaud
Malonne, Hugues
Poels, Jeroen
André, Emmanuel
author_sort Van Vooren, Steven
collection PubMed
description We present our approach to rapidly establishing a standardized, multi-site, nation-wide COVID-19 screening program in Belgium. Under auspices of a federal government Task Force responsible for upscaling the country’s testing capacity, we were able to set up a national testing initiative with readily available resources, putting in place a robust, validated, high-throughput, and decentralized qPCR molecular testing platform with embedded proficiency testing. We demonstrate how during an acute scarcity of equipment, kits, reagents, personnel, protective equipment, and sterile plastic supplies, we introduced an approach to rapidly build a reliable, validated, high-volume, high-confidence workflow based on heterogeneous instrumentation and diverse assays, assay components, and protocols. The workflow was set up with continuous quality control monitoring, tied together through a clinical-grade information management platform for automated data analysis, real-time result reporting across different participating sites, qc monitoring, and making result data available to the requesting physician and the patient. In this overview, we address challenges in optimizing high-throughput cross-laboratory workflows with minimal manual intervention through software, instrument and assay validation and standardization, and a process for harmonized result reporting and nation-level infection statistics monitoring across the disparate testing methodologies and workflows, necessitated by a rapid scale-up as a response to the pandemic.
format Online
Article
Text
id pubmed-8879918
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-88799182022-02-26 Reliable and Scalable SARS-CoV-2 qPCR Testing at a High Sample Throughput: Lessons Learned from the Belgian Initiative Van Vooren, Steven Grayson, James Van Ranst, Marc Dequeker, Elisabeth Laenen, Lies Janssen, Reile Gillet, Laurent Bureau, Fabrice Coppieters, Wouter Devos, Nathalie Hengchen, Benjamin Wattiau, Pierre Méhauden, Sibylle Verlinden, Yvan Van Baelen, Kurt Pattery, Theresa Valentin, Jean-Pierre Janssen, Kris Geraerts, Martine Smeraglia, John Hellemans, Jan Wytynck, Pieter Mestdagh, Pieter Besbrugge, Nienke Höfer, René Nollet, Friedel Vandesompele, Jo De Smet, Pieter Lebon, John Vandewynckele, Emmanuel Verstrepen, Steven Uten, Wouter Capron, Arnaud Malonne, Hugues Poels, Jeroen André, Emmanuel Life (Basel) Article We present our approach to rapidly establishing a standardized, multi-site, nation-wide COVID-19 screening program in Belgium. Under auspices of a federal government Task Force responsible for upscaling the country’s testing capacity, we were able to set up a national testing initiative with readily available resources, putting in place a robust, validated, high-throughput, and decentralized qPCR molecular testing platform with embedded proficiency testing. We demonstrate how during an acute scarcity of equipment, kits, reagents, personnel, protective equipment, and sterile plastic supplies, we introduced an approach to rapidly build a reliable, validated, high-volume, high-confidence workflow based on heterogeneous instrumentation and diverse assays, assay components, and protocols. The workflow was set up with continuous quality control monitoring, tied together through a clinical-grade information management platform for automated data analysis, real-time result reporting across different participating sites, qc monitoring, and making result data available to the requesting physician and the patient. In this overview, we address challenges in optimizing high-throughput cross-laboratory workflows with minimal manual intervention through software, instrument and assay validation and standardization, and a process for harmonized result reporting and nation-level infection statistics monitoring across the disparate testing methodologies and workflows, necessitated by a rapid scale-up as a response to the pandemic. MDPI 2022-01-21 /pmc/articles/PMC8879918/ /pubmed/35207446 http://dx.doi.org/10.3390/life12020159 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Van Vooren, Steven
Grayson, James
Van Ranst, Marc
Dequeker, Elisabeth
Laenen, Lies
Janssen, Reile
Gillet, Laurent
Bureau, Fabrice
Coppieters, Wouter
Devos, Nathalie
Hengchen, Benjamin
Wattiau, Pierre
Méhauden, Sibylle
Verlinden, Yvan
Van Baelen, Kurt
Pattery, Theresa
Valentin, Jean-Pierre
Janssen, Kris
Geraerts, Martine
Smeraglia, John
Hellemans, Jan
Wytynck, Pieter
Mestdagh, Pieter
Besbrugge, Nienke
Höfer, René
Nollet, Friedel
Vandesompele, Jo
De Smet, Pieter
Lebon, John
Vandewynckele, Emmanuel
Verstrepen, Steven
Uten, Wouter
Capron, Arnaud
Malonne, Hugues
Poels, Jeroen
André, Emmanuel
Reliable and Scalable SARS-CoV-2 qPCR Testing at a High Sample Throughput: Lessons Learned from the Belgian Initiative
title Reliable and Scalable SARS-CoV-2 qPCR Testing at a High Sample Throughput: Lessons Learned from the Belgian Initiative
title_full Reliable and Scalable SARS-CoV-2 qPCR Testing at a High Sample Throughput: Lessons Learned from the Belgian Initiative
title_fullStr Reliable and Scalable SARS-CoV-2 qPCR Testing at a High Sample Throughput: Lessons Learned from the Belgian Initiative
title_full_unstemmed Reliable and Scalable SARS-CoV-2 qPCR Testing at a High Sample Throughput: Lessons Learned from the Belgian Initiative
title_short Reliable and Scalable SARS-CoV-2 qPCR Testing at a High Sample Throughput: Lessons Learned from the Belgian Initiative
title_sort reliable and scalable sars-cov-2 qpcr testing at a high sample throughput: lessons learned from the belgian initiative
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8879918/
https://www.ncbi.nlm.nih.gov/pubmed/35207446
http://dx.doi.org/10.3390/life12020159
work_keys_str_mv AT vanvoorensteven reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT graysonjames reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT vanranstmarc reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT dequekerelisabeth reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT laenenlies reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT janssenreile reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT gilletlaurent reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT bureaufabrice reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT coppieterswouter reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT devosnathalie reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT hengchenbenjamin reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT wattiaupierre reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT mehaudensibylle reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT verlindenyvan reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT vanbaelenkurt reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT patterytheresa reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT valentinjeanpierre reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT janssenkris reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT geraertsmartine reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT smeragliajohn reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT hellemansjan reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT wytynckpieter reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT mestdaghpieter reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT besbruggenienke reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT hoferrene reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT nolletfriedel reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT vandesompelejo reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT desmetpieter reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT lebonjohn reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT vandewynckeleemmanuel reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT verstrepensteven reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT utenwouter reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT capronarnaud reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT malonnehugues reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT poelsjeroen reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative
AT andreemmanuel reliableandscalablesarscov2qpcrtestingatahighsamplethroughputlessonslearnedfromthebelgianinitiative