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The PAP Gene Family in Tomato: Comprehensive Comparative Analysis, Phylogenetic Relationships and Expression Profiles

Purple acid phosphatase (PAP) plays a vital role in plant phosphate acquisition and utilization, as well as cell wall synthesis and redox reactions. In this study, comprehensive comparative analyses of PAP genes were carried out using the integration of phylogeny, chromosomal localization, intron/ex...

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Autores principales: Pang, Xin, Cheng, Yuan, Ruan, Meiying, Ye, Qingjing, Wang, Rongqing, Yao, Zhuping, Zhou, Guozhi, Wan, Hongjian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8879926/
https://www.ncbi.nlm.nih.gov/pubmed/35214896
http://dx.doi.org/10.3390/plants11040563
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author Pang, Xin
Cheng, Yuan
Ruan, Meiying
Ye, Qingjing
Wang, Rongqing
Yao, Zhuping
Zhou, Guozhi
Wan, Hongjian
author_facet Pang, Xin
Cheng, Yuan
Ruan, Meiying
Ye, Qingjing
Wang, Rongqing
Yao, Zhuping
Zhou, Guozhi
Wan, Hongjian
author_sort Pang, Xin
collection PubMed
description Purple acid phosphatase (PAP) plays a vital role in plant phosphate acquisition and utilization, as well as cell wall synthesis and redox reactions. In this study, comprehensive comparative analyses of PAP genes were carried out using the integration of phylogeny, chromosomal localization, intron/exon structural characteristics, and expression profiling. It was shown that the number of introns of the PAP genes, which were distributed unevenly on 12 chromosomes, ranged from 1 to 12. These findings pointed to the existence of complex structures. Phylogenetic analyses revealed that PAPs from tomato, rice, and Arabidopsis could be divided into three groups (Groups I, II, and III). It was assumed that the diversity of these PAP genes occurred before the monocot–dicot split. RNA-seq analysis revealed that most of the genes were expressed in all of the tissues analyzed, with the exception of SlPAP02, SlPAP11, and SlPAP14, which were not detected. It was also found that expression levels of most of the SlPAP gene family of members were changed under phosphorus stress conditions, suggesting potential functional diversification. The findings of this work will help us to achieve a better insight into the function of SlPAP genes in the future, as well as enhance our understanding of their evolutionary relationships in plants.
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spelling pubmed-88799262022-02-26 The PAP Gene Family in Tomato: Comprehensive Comparative Analysis, Phylogenetic Relationships and Expression Profiles Pang, Xin Cheng, Yuan Ruan, Meiying Ye, Qingjing Wang, Rongqing Yao, Zhuping Zhou, Guozhi Wan, Hongjian Plants (Basel) Article Purple acid phosphatase (PAP) plays a vital role in plant phosphate acquisition and utilization, as well as cell wall synthesis and redox reactions. In this study, comprehensive comparative analyses of PAP genes were carried out using the integration of phylogeny, chromosomal localization, intron/exon structural characteristics, and expression profiling. It was shown that the number of introns of the PAP genes, which were distributed unevenly on 12 chromosomes, ranged from 1 to 12. These findings pointed to the existence of complex structures. Phylogenetic analyses revealed that PAPs from tomato, rice, and Arabidopsis could be divided into three groups (Groups I, II, and III). It was assumed that the diversity of these PAP genes occurred before the monocot–dicot split. RNA-seq analysis revealed that most of the genes were expressed in all of the tissues analyzed, with the exception of SlPAP02, SlPAP11, and SlPAP14, which were not detected. It was also found that expression levels of most of the SlPAP gene family of members were changed under phosphorus stress conditions, suggesting potential functional diversification. The findings of this work will help us to achieve a better insight into the function of SlPAP genes in the future, as well as enhance our understanding of their evolutionary relationships in plants. MDPI 2022-02-21 /pmc/articles/PMC8879926/ /pubmed/35214896 http://dx.doi.org/10.3390/plants11040563 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pang, Xin
Cheng, Yuan
Ruan, Meiying
Ye, Qingjing
Wang, Rongqing
Yao, Zhuping
Zhou, Guozhi
Wan, Hongjian
The PAP Gene Family in Tomato: Comprehensive Comparative Analysis, Phylogenetic Relationships and Expression Profiles
title The PAP Gene Family in Tomato: Comprehensive Comparative Analysis, Phylogenetic Relationships and Expression Profiles
title_full The PAP Gene Family in Tomato: Comprehensive Comparative Analysis, Phylogenetic Relationships and Expression Profiles
title_fullStr The PAP Gene Family in Tomato: Comprehensive Comparative Analysis, Phylogenetic Relationships and Expression Profiles
title_full_unstemmed The PAP Gene Family in Tomato: Comprehensive Comparative Analysis, Phylogenetic Relationships and Expression Profiles
title_short The PAP Gene Family in Tomato: Comprehensive Comparative Analysis, Phylogenetic Relationships and Expression Profiles
title_sort pap gene family in tomato: comprehensive comparative analysis, phylogenetic relationships and expression profiles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8879926/
https://www.ncbi.nlm.nih.gov/pubmed/35214896
http://dx.doi.org/10.3390/plants11040563
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