Cargando…

A Target-Site Mutation Confers Cross-Resistance to ALS-Inhibiting Herbicides in Erigeron sumatrensis from Brazil

Cases of weed resistant to herbicides have changed the dynamics of agricultural areas in Brazil, and in recent years, Erigeron species have caused major problems to farmers in the country, mainly in relation to the ineffectiveness of herbicide treatments used. The objective of this study was to conf...

Descripción completa

Detalles Bibliográficos
Autores principales: Vital Silva, Vanessa, Mendes, Rafael, Suzukawa, Andreia, Adegas, Fernando, Marcelino-Guimaraes, Francismar, Oliveira, Rubem
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8879965/
https://www.ncbi.nlm.nih.gov/pubmed/35214801
http://dx.doi.org/10.3390/plants11040467
_version_ 1784659047859879936
author Vital Silva, Vanessa
Mendes, Rafael
Suzukawa, Andreia
Adegas, Fernando
Marcelino-Guimaraes, Francismar
Oliveira, Rubem
author_facet Vital Silva, Vanessa
Mendes, Rafael
Suzukawa, Andreia
Adegas, Fernando
Marcelino-Guimaraes, Francismar
Oliveira, Rubem
author_sort Vital Silva, Vanessa
collection PubMed
description Cases of weed resistant to herbicides have changed the dynamics of agricultural areas in Brazil, and in recent years, Erigeron species have caused major problems to farmers in the country, mainly in relation to the ineffectiveness of herbicide treatments used. The objective of this study was to confirm the cross-resistance to ALS inhibitors in populations of Erigeron sumatrensis as well as to investigate the existence of mutations in the site of action of ALS-inhibiting herbicides. To do this, 30 populations collected in the 2016/2017 crop season were grown in a greenhouse. Dose–response (chlorimuron-ethyl and cloransulam-methyl), inhibition of cytochrome P-450 with malathion, and ALS gene sequencing experiments were carried out in the F1 generations of two fleabane populations. The results proved the cross-resistance to chlorimuron-ethyl and cloransulam-methyl herbicides applied in the post-emergence of the resistant population of E. sumatrensis. The higher activity of P450 enzymes is unlikely responsible for the resistance of the population studied. The resistance mechanism found in R was the target site mutation Pro197Ser at the ALS gene. This is the first study in Brazil to identify a target-site change as a survival mechanism in E. sumatrensis for the resistance to ALS-inhibiting herbicides.
format Online
Article
Text
id pubmed-8879965
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-88799652022-02-26 A Target-Site Mutation Confers Cross-Resistance to ALS-Inhibiting Herbicides in Erigeron sumatrensis from Brazil Vital Silva, Vanessa Mendes, Rafael Suzukawa, Andreia Adegas, Fernando Marcelino-Guimaraes, Francismar Oliveira, Rubem Plants (Basel) Article Cases of weed resistant to herbicides have changed the dynamics of agricultural areas in Brazil, and in recent years, Erigeron species have caused major problems to farmers in the country, mainly in relation to the ineffectiveness of herbicide treatments used. The objective of this study was to confirm the cross-resistance to ALS inhibitors in populations of Erigeron sumatrensis as well as to investigate the existence of mutations in the site of action of ALS-inhibiting herbicides. To do this, 30 populations collected in the 2016/2017 crop season were grown in a greenhouse. Dose–response (chlorimuron-ethyl and cloransulam-methyl), inhibition of cytochrome P-450 with malathion, and ALS gene sequencing experiments were carried out in the F1 generations of two fleabane populations. The results proved the cross-resistance to chlorimuron-ethyl and cloransulam-methyl herbicides applied in the post-emergence of the resistant population of E. sumatrensis. The higher activity of P450 enzymes is unlikely responsible for the resistance of the population studied. The resistance mechanism found in R was the target site mutation Pro197Ser at the ALS gene. This is the first study in Brazil to identify a target-site change as a survival mechanism in E. sumatrensis for the resistance to ALS-inhibiting herbicides. MDPI 2022-02-09 /pmc/articles/PMC8879965/ /pubmed/35214801 http://dx.doi.org/10.3390/plants11040467 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vital Silva, Vanessa
Mendes, Rafael
Suzukawa, Andreia
Adegas, Fernando
Marcelino-Guimaraes, Francismar
Oliveira, Rubem
A Target-Site Mutation Confers Cross-Resistance to ALS-Inhibiting Herbicides in Erigeron sumatrensis from Brazil
title A Target-Site Mutation Confers Cross-Resistance to ALS-Inhibiting Herbicides in Erigeron sumatrensis from Brazil
title_full A Target-Site Mutation Confers Cross-Resistance to ALS-Inhibiting Herbicides in Erigeron sumatrensis from Brazil
title_fullStr A Target-Site Mutation Confers Cross-Resistance to ALS-Inhibiting Herbicides in Erigeron sumatrensis from Brazil
title_full_unstemmed A Target-Site Mutation Confers Cross-Resistance to ALS-Inhibiting Herbicides in Erigeron sumatrensis from Brazil
title_short A Target-Site Mutation Confers Cross-Resistance to ALS-Inhibiting Herbicides in Erigeron sumatrensis from Brazil
title_sort target-site mutation confers cross-resistance to als-inhibiting herbicides in erigeron sumatrensis from brazil
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8879965/
https://www.ncbi.nlm.nih.gov/pubmed/35214801
http://dx.doi.org/10.3390/plants11040467
work_keys_str_mv AT vitalsilvavanessa atargetsitemutationconferscrossresistancetoalsinhibitingherbicidesinerigeronsumatrensisfrombrazil
AT mendesrafael atargetsitemutationconferscrossresistancetoalsinhibitingherbicidesinerigeronsumatrensisfrombrazil
AT suzukawaandreia atargetsitemutationconferscrossresistancetoalsinhibitingherbicidesinerigeronsumatrensisfrombrazil
AT adegasfernando atargetsitemutationconferscrossresistancetoalsinhibitingherbicidesinerigeronsumatrensisfrombrazil
AT marcelinoguimaraesfrancismar atargetsitemutationconferscrossresistancetoalsinhibitingherbicidesinerigeronsumatrensisfrombrazil
AT oliveirarubem atargetsitemutationconferscrossresistancetoalsinhibitingherbicidesinerigeronsumatrensisfrombrazil
AT vitalsilvavanessa targetsitemutationconferscrossresistancetoalsinhibitingherbicidesinerigeronsumatrensisfrombrazil
AT mendesrafael targetsitemutationconferscrossresistancetoalsinhibitingherbicidesinerigeronsumatrensisfrombrazil
AT suzukawaandreia targetsitemutationconferscrossresistancetoalsinhibitingherbicidesinerigeronsumatrensisfrombrazil
AT adegasfernando targetsitemutationconferscrossresistancetoalsinhibitingherbicidesinerigeronsumatrensisfrombrazil
AT marcelinoguimaraesfrancismar targetsitemutationconferscrossresistancetoalsinhibitingherbicidesinerigeronsumatrensisfrombrazil
AT oliveirarubem targetsitemutationconferscrossresistancetoalsinhibitingherbicidesinerigeronsumatrensisfrombrazil