Application of the CRISPR/Cas9 System to Study Regulation Pathways of the Cellular Immune Response to Influenza Virus

Influenza A virus (IAV) causes a respiratory infection that affects millions of people of different age groups and can lead to acute respiratory distress syndrome. Currently, host genes, receptors, and other cellular components critical for IAV replication are actively studied. One of the most conve...

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Autores principales: Prokhorova, Daria, Zhukova (Eschenko), Natalya, Lemza, Anna, Sergeeva, Mariia, Amirkhanov, Rinat, Stepanov, Grigory
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8879999/
https://www.ncbi.nlm.nih.gov/pubmed/35216030
http://dx.doi.org/10.3390/v14020437
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author Prokhorova, Daria
Zhukova (Eschenko), Natalya
Lemza, Anna
Sergeeva, Mariia
Amirkhanov, Rinat
Stepanov, Grigory
author_facet Prokhorova, Daria
Zhukova (Eschenko), Natalya
Lemza, Anna
Sergeeva, Mariia
Amirkhanov, Rinat
Stepanov, Grigory
author_sort Prokhorova, Daria
collection PubMed
description Influenza A virus (IAV) causes a respiratory infection that affects millions of people of different age groups and can lead to acute respiratory distress syndrome. Currently, host genes, receptors, and other cellular components critical for IAV replication are actively studied. One of the most convenient and accessible genome-editing tools to facilitate these studies is the CRISPR/Cas9 system. This tool allows for regulating the expression of both viral and host cell genes to enhance or impair viral entry and replication. This review considers the effect of the genome editing system on specific target genes in cells (human and chicken) in terms of subsequent changes in the influenza virus life cycle and the efficiency of virus particle production.
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spelling pubmed-88799992022-02-26 Application of the CRISPR/Cas9 System to Study Regulation Pathways of the Cellular Immune Response to Influenza Virus Prokhorova, Daria Zhukova (Eschenko), Natalya Lemza, Anna Sergeeva, Mariia Amirkhanov, Rinat Stepanov, Grigory Viruses Review Influenza A virus (IAV) causes a respiratory infection that affects millions of people of different age groups and can lead to acute respiratory distress syndrome. Currently, host genes, receptors, and other cellular components critical for IAV replication are actively studied. One of the most convenient and accessible genome-editing tools to facilitate these studies is the CRISPR/Cas9 system. This tool allows for regulating the expression of both viral and host cell genes to enhance or impair viral entry and replication. This review considers the effect of the genome editing system on specific target genes in cells (human and chicken) in terms of subsequent changes in the influenza virus life cycle and the efficiency of virus particle production. MDPI 2022-02-21 /pmc/articles/PMC8879999/ /pubmed/35216030 http://dx.doi.org/10.3390/v14020437 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Prokhorova, Daria
Zhukova (Eschenko), Natalya
Lemza, Anna
Sergeeva, Mariia
Amirkhanov, Rinat
Stepanov, Grigory
Application of the CRISPR/Cas9 System to Study Regulation Pathways of the Cellular Immune Response to Influenza Virus
title Application of the CRISPR/Cas9 System to Study Regulation Pathways of the Cellular Immune Response to Influenza Virus
title_full Application of the CRISPR/Cas9 System to Study Regulation Pathways of the Cellular Immune Response to Influenza Virus
title_fullStr Application of the CRISPR/Cas9 System to Study Regulation Pathways of the Cellular Immune Response to Influenza Virus
title_full_unstemmed Application of the CRISPR/Cas9 System to Study Regulation Pathways of the Cellular Immune Response to Influenza Virus
title_short Application of the CRISPR/Cas9 System to Study Regulation Pathways of the Cellular Immune Response to Influenza Virus
title_sort application of the crispr/cas9 system to study regulation pathways of the cellular immune response to influenza virus
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8879999/
https://www.ncbi.nlm.nih.gov/pubmed/35216030
http://dx.doi.org/10.3390/v14020437
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