Cargando…

In Situ Agarose Microfabrication Technology Using Joule Heating of Micro Ionic Current for On-Chip Cell Network Analysis

Agarose microfabrication technology is one of the micropatterning techniques of cells having advantages of simple and flexible real-time fabrication of three-dimensional confinement microstructures even during cell cultivation. However, the conventional photothermal etching procedure of focused infr...

Descripción completa

Detalles Bibliográficos
Autores principales: Shimoda, Kenji, Watanabe, Haruki, Hondo, Yoshitsune, Sentoku, Mitsuru, Sakamoto, Kazufumi, Yasuda, Kenji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8880086/
https://www.ncbi.nlm.nih.gov/pubmed/35208299
http://dx.doi.org/10.3390/mi13020174
_version_ 1784659090997248000
author Shimoda, Kenji
Watanabe, Haruki
Hondo, Yoshitsune
Sentoku, Mitsuru
Sakamoto, Kazufumi
Yasuda, Kenji
author_facet Shimoda, Kenji
Watanabe, Haruki
Hondo, Yoshitsune
Sentoku, Mitsuru
Sakamoto, Kazufumi
Yasuda, Kenji
author_sort Shimoda, Kenji
collection PubMed
description Agarose microfabrication technology is one of the micropatterning techniques of cells having advantages of simple and flexible real-time fabrication of three-dimensional confinement microstructures even during cell cultivation. However, the conventional photothermal etching procedure of focused infrared laser on thin agarose layer has several limitations, such as the undesired sudden change of etched width caused by the local change of absorbance of the bottom surface of cultivation plate, especially on the indium-tin-oxide (ITO) wiring on the multi-electrode array (MEA) cultivation chip. To overcome these limitations, we have developed a new agarose etching method exploiting the Joule heating of focused micro ionic current at the tip of the micrometer-sized capillary tube. When 75 V, 1 kHz AC voltage was applied to the tapered microcapillary tube, in which 1 M sodium ion buffer was filled, the formed micro ionic current at the open end of the microcapillary tube melted the thin agarose layer and formed stable 5 μm width microstructures regardless the ITO wiring, and the width was controlled by the change of applied voltage squared. We also found the importance of the higher frequency of applied AC voltage to form the stable microstructures and also minimize the fluctuation of melted width. The results indicate that the focused micro ionic current can create stable local spot heating in the medium buffer as the Joule heating of local ionic current and can perform the same quality of microfabrication as the focused infrared laser absorption procedure with a simple set-up of the system and several advantages.
format Online
Article
Text
id pubmed-8880086
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-88800862022-02-26 In Situ Agarose Microfabrication Technology Using Joule Heating of Micro Ionic Current for On-Chip Cell Network Analysis Shimoda, Kenji Watanabe, Haruki Hondo, Yoshitsune Sentoku, Mitsuru Sakamoto, Kazufumi Yasuda, Kenji Micromachines (Basel) Article Agarose microfabrication technology is one of the micropatterning techniques of cells having advantages of simple and flexible real-time fabrication of three-dimensional confinement microstructures even during cell cultivation. However, the conventional photothermal etching procedure of focused infrared laser on thin agarose layer has several limitations, such as the undesired sudden change of etched width caused by the local change of absorbance of the bottom surface of cultivation plate, especially on the indium-tin-oxide (ITO) wiring on the multi-electrode array (MEA) cultivation chip. To overcome these limitations, we have developed a new agarose etching method exploiting the Joule heating of focused micro ionic current at the tip of the micrometer-sized capillary tube. When 75 V, 1 kHz AC voltage was applied to the tapered microcapillary tube, in which 1 M sodium ion buffer was filled, the formed micro ionic current at the open end of the microcapillary tube melted the thin agarose layer and formed stable 5 μm width microstructures regardless the ITO wiring, and the width was controlled by the change of applied voltage squared. We also found the importance of the higher frequency of applied AC voltage to form the stable microstructures and also minimize the fluctuation of melted width. The results indicate that the focused micro ionic current can create stable local spot heating in the medium buffer as the Joule heating of local ionic current and can perform the same quality of microfabrication as the focused infrared laser absorption procedure with a simple set-up of the system and several advantages. MDPI 2022-01-25 /pmc/articles/PMC8880086/ /pubmed/35208299 http://dx.doi.org/10.3390/mi13020174 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shimoda, Kenji
Watanabe, Haruki
Hondo, Yoshitsune
Sentoku, Mitsuru
Sakamoto, Kazufumi
Yasuda, Kenji
In Situ Agarose Microfabrication Technology Using Joule Heating of Micro Ionic Current for On-Chip Cell Network Analysis
title In Situ Agarose Microfabrication Technology Using Joule Heating of Micro Ionic Current for On-Chip Cell Network Analysis
title_full In Situ Agarose Microfabrication Technology Using Joule Heating of Micro Ionic Current for On-Chip Cell Network Analysis
title_fullStr In Situ Agarose Microfabrication Technology Using Joule Heating of Micro Ionic Current for On-Chip Cell Network Analysis
title_full_unstemmed In Situ Agarose Microfabrication Technology Using Joule Heating of Micro Ionic Current for On-Chip Cell Network Analysis
title_short In Situ Agarose Microfabrication Technology Using Joule Heating of Micro Ionic Current for On-Chip Cell Network Analysis
title_sort in situ agarose microfabrication technology using joule heating of micro ionic current for on-chip cell network analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8880086/
https://www.ncbi.nlm.nih.gov/pubmed/35208299
http://dx.doi.org/10.3390/mi13020174
work_keys_str_mv AT shimodakenji insituagarosemicrofabricationtechnologyusingjouleheatingofmicroioniccurrentforonchipcellnetworkanalysis
AT watanabeharuki insituagarosemicrofabricationtechnologyusingjouleheatingofmicroioniccurrentforonchipcellnetworkanalysis
AT hondoyoshitsune insituagarosemicrofabricationtechnologyusingjouleheatingofmicroioniccurrentforonchipcellnetworkanalysis
AT sentokumitsuru insituagarosemicrofabricationtechnologyusingjouleheatingofmicroioniccurrentforonchipcellnetworkanalysis
AT sakamotokazufumi insituagarosemicrofabricationtechnologyusingjouleheatingofmicroioniccurrentforonchipcellnetworkanalysis
AT yasudakenji insituagarosemicrofabricationtechnologyusingjouleheatingofmicroioniccurrentforonchipcellnetworkanalysis