Cargando…
The Accuracy of Serum Biomarkers in the Diagnosis of Steatosis, Fibrosis, and Inflammation in Patients with Nonalcoholic Fatty Liver Disease in Comparison to a Liver Biopsy
Background and Objective: This study was conducted to evaluate the diagnostic performance of various biomarkers for steatosis, fibrosis, and inflammation in comparison to a liver biopsy (LB) in patients with nonalcoholic fatty liver disease (NAFLD). Materials and Methods: This was a cross-sectional...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8880154/ https://www.ncbi.nlm.nih.gov/pubmed/35208576 http://dx.doi.org/10.3390/medicina58020252 |
_version_ | 1784659109352570880 |
---|---|
author | Mikolasevic, Ivana Domislovic, Viktor Krznaric-Zrnic, Irena Krznaric, Zeljko Virovic-Jukic, Lucija Stojsavljevic, Sanja Grgurevic, Ivica Milic, Sandra Vukoja, Ivan Puz, Petra Aralica, Merica Hauser, Goran |
author_facet | Mikolasevic, Ivana Domislovic, Viktor Krznaric-Zrnic, Irena Krznaric, Zeljko Virovic-Jukic, Lucija Stojsavljevic, Sanja Grgurevic, Ivica Milic, Sandra Vukoja, Ivan Puz, Petra Aralica, Merica Hauser, Goran |
author_sort | Mikolasevic, Ivana |
collection | PubMed |
description | Background and Objective: This study was conducted to evaluate the diagnostic performance of various biomarkers for steatosis, fibrosis, and inflammation in comparison to a liver biopsy (LB) in patients with nonalcoholic fatty liver disease (NAFLD). Materials and Methods: This was a cross-sectional study that included 135 patients with biopsy-proven NAFLD. Fatty liver index (FLI), hepatic steatosis index (HSI), cell death markers (CK-18 M30 and CK-18 M65), FIB-4 index, NAFLD fibrosis score (NFS), BARD, and AST to platelet ratio index (APRI) were calculated and analysed. Results: FLI, HSI scores, and the cell death biomarkers showed poor diagnostic accuracy for steatosis detection and quantification, with an area under the curve (AUC) of <0.70. The cell death biomarkers likewise did not perform well for the detection of nonalcoholic steatohepatitis (NASH) (AUC < 0.7). As for the fibrosis staging, only APRI and the cell death biomarkers had moderate accuracy (AUC > 0.7) for advanced fibrosis, whereas FIB-4, BARD, and NFS scores demonstrated poor performance (AUC < 0.70). However, a combination of FIB-4 and NFS with the cell death biomarkers had moderate accuracy for advanced (≥F3) fibrosis detection, with an AUC of >0.70. Conclusions: In this first study on Croatian patients with NAFLD, serum biomarkers demonstrated poor diagnostic performance for the noninvasive diagnosis of liver steatosis and NASH. APRI and the cell death biomarkers had only moderate accuracy for diagnosing advanced fibrosis, as did the combination of FIB-4 and NFS with the cell death biomarkers. Further studies regarding serum biomarkers for all NAFLD stages are needed. |
format | Online Article Text |
id | pubmed-8880154 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88801542022-02-26 The Accuracy of Serum Biomarkers in the Diagnosis of Steatosis, Fibrosis, and Inflammation in Patients with Nonalcoholic Fatty Liver Disease in Comparison to a Liver Biopsy Mikolasevic, Ivana Domislovic, Viktor Krznaric-Zrnic, Irena Krznaric, Zeljko Virovic-Jukic, Lucija Stojsavljevic, Sanja Grgurevic, Ivica Milic, Sandra Vukoja, Ivan Puz, Petra Aralica, Merica Hauser, Goran Medicina (Kaunas) Article Background and Objective: This study was conducted to evaluate the diagnostic performance of various biomarkers for steatosis, fibrosis, and inflammation in comparison to a liver biopsy (LB) in patients with nonalcoholic fatty liver disease (NAFLD). Materials and Methods: This was a cross-sectional study that included 135 patients with biopsy-proven NAFLD. Fatty liver index (FLI), hepatic steatosis index (HSI), cell death markers (CK-18 M30 and CK-18 M65), FIB-4 index, NAFLD fibrosis score (NFS), BARD, and AST to platelet ratio index (APRI) were calculated and analysed. Results: FLI, HSI scores, and the cell death biomarkers showed poor diagnostic accuracy for steatosis detection and quantification, with an area under the curve (AUC) of <0.70. The cell death biomarkers likewise did not perform well for the detection of nonalcoholic steatohepatitis (NASH) (AUC < 0.7). As for the fibrosis staging, only APRI and the cell death biomarkers had moderate accuracy (AUC > 0.7) for advanced fibrosis, whereas FIB-4, BARD, and NFS scores demonstrated poor performance (AUC < 0.70). However, a combination of FIB-4 and NFS with the cell death biomarkers had moderate accuracy for advanced (≥F3) fibrosis detection, with an AUC of >0.70. Conclusions: In this first study on Croatian patients with NAFLD, serum biomarkers demonstrated poor diagnostic performance for the noninvasive diagnosis of liver steatosis and NASH. APRI and the cell death biomarkers had only moderate accuracy for diagnosing advanced fibrosis, as did the combination of FIB-4 and NFS with the cell death biomarkers. Further studies regarding serum biomarkers for all NAFLD stages are needed. MDPI 2022-02-08 /pmc/articles/PMC8880154/ /pubmed/35208576 http://dx.doi.org/10.3390/medicina58020252 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mikolasevic, Ivana Domislovic, Viktor Krznaric-Zrnic, Irena Krznaric, Zeljko Virovic-Jukic, Lucija Stojsavljevic, Sanja Grgurevic, Ivica Milic, Sandra Vukoja, Ivan Puz, Petra Aralica, Merica Hauser, Goran The Accuracy of Serum Biomarkers in the Diagnosis of Steatosis, Fibrosis, and Inflammation in Patients with Nonalcoholic Fatty Liver Disease in Comparison to a Liver Biopsy |
title | The Accuracy of Serum Biomarkers in the Diagnosis of Steatosis, Fibrosis, and Inflammation in Patients with Nonalcoholic Fatty Liver Disease in Comparison to a Liver Biopsy |
title_full | The Accuracy of Serum Biomarkers in the Diagnosis of Steatosis, Fibrosis, and Inflammation in Patients with Nonalcoholic Fatty Liver Disease in Comparison to a Liver Biopsy |
title_fullStr | The Accuracy of Serum Biomarkers in the Diagnosis of Steatosis, Fibrosis, and Inflammation in Patients with Nonalcoholic Fatty Liver Disease in Comparison to a Liver Biopsy |
title_full_unstemmed | The Accuracy of Serum Biomarkers in the Diagnosis of Steatosis, Fibrosis, and Inflammation in Patients with Nonalcoholic Fatty Liver Disease in Comparison to a Liver Biopsy |
title_short | The Accuracy of Serum Biomarkers in the Diagnosis of Steatosis, Fibrosis, and Inflammation in Patients with Nonalcoholic Fatty Liver Disease in Comparison to a Liver Biopsy |
title_sort | accuracy of serum biomarkers in the diagnosis of steatosis, fibrosis, and inflammation in patients with nonalcoholic fatty liver disease in comparison to a liver biopsy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8880154/ https://www.ncbi.nlm.nih.gov/pubmed/35208576 http://dx.doi.org/10.3390/medicina58020252 |
work_keys_str_mv | AT mikolasevicivana theaccuracyofserumbiomarkersinthediagnosisofsteatosisfibrosisandinflammationinpatientswithnonalcoholicfattyliverdiseaseincomparisontoaliverbiopsy AT domislovicviktor theaccuracyofserumbiomarkersinthediagnosisofsteatosisfibrosisandinflammationinpatientswithnonalcoholicfattyliverdiseaseincomparisontoaliverbiopsy AT krznariczrnicirena theaccuracyofserumbiomarkersinthediagnosisofsteatosisfibrosisandinflammationinpatientswithnonalcoholicfattyliverdiseaseincomparisontoaliverbiopsy AT krznariczeljko theaccuracyofserumbiomarkersinthediagnosisofsteatosisfibrosisandinflammationinpatientswithnonalcoholicfattyliverdiseaseincomparisontoaliverbiopsy AT virovicjukiclucija theaccuracyofserumbiomarkersinthediagnosisofsteatosisfibrosisandinflammationinpatientswithnonalcoholicfattyliverdiseaseincomparisontoaliverbiopsy AT stojsavljevicsanja theaccuracyofserumbiomarkersinthediagnosisofsteatosisfibrosisandinflammationinpatientswithnonalcoholicfattyliverdiseaseincomparisontoaliverbiopsy AT grgurevicivica theaccuracyofserumbiomarkersinthediagnosisofsteatosisfibrosisandinflammationinpatientswithnonalcoholicfattyliverdiseaseincomparisontoaliverbiopsy AT milicsandra theaccuracyofserumbiomarkersinthediagnosisofsteatosisfibrosisandinflammationinpatientswithnonalcoholicfattyliverdiseaseincomparisontoaliverbiopsy AT vukojaivan theaccuracyofserumbiomarkersinthediagnosisofsteatosisfibrosisandinflammationinpatientswithnonalcoholicfattyliverdiseaseincomparisontoaliverbiopsy AT puzpetra theaccuracyofserumbiomarkersinthediagnosisofsteatosisfibrosisandinflammationinpatientswithnonalcoholicfattyliverdiseaseincomparisontoaliverbiopsy AT aralicamerica theaccuracyofserumbiomarkersinthediagnosisofsteatosisfibrosisandinflammationinpatientswithnonalcoholicfattyliverdiseaseincomparisontoaliverbiopsy AT hausergoran theaccuracyofserumbiomarkersinthediagnosisofsteatosisfibrosisandinflammationinpatientswithnonalcoholicfattyliverdiseaseincomparisontoaliverbiopsy AT mikolasevicivana accuracyofserumbiomarkersinthediagnosisofsteatosisfibrosisandinflammationinpatientswithnonalcoholicfattyliverdiseaseincomparisontoaliverbiopsy AT domislovicviktor accuracyofserumbiomarkersinthediagnosisofsteatosisfibrosisandinflammationinpatientswithnonalcoholicfattyliverdiseaseincomparisontoaliverbiopsy AT krznariczrnicirena accuracyofserumbiomarkersinthediagnosisofsteatosisfibrosisandinflammationinpatientswithnonalcoholicfattyliverdiseaseincomparisontoaliverbiopsy AT krznariczeljko accuracyofserumbiomarkersinthediagnosisofsteatosisfibrosisandinflammationinpatientswithnonalcoholicfattyliverdiseaseincomparisontoaliverbiopsy AT virovicjukiclucija accuracyofserumbiomarkersinthediagnosisofsteatosisfibrosisandinflammationinpatientswithnonalcoholicfattyliverdiseaseincomparisontoaliverbiopsy AT stojsavljevicsanja accuracyofserumbiomarkersinthediagnosisofsteatosisfibrosisandinflammationinpatientswithnonalcoholicfattyliverdiseaseincomparisontoaliverbiopsy AT grgurevicivica accuracyofserumbiomarkersinthediagnosisofsteatosisfibrosisandinflammationinpatientswithnonalcoholicfattyliverdiseaseincomparisontoaliverbiopsy AT milicsandra accuracyofserumbiomarkersinthediagnosisofsteatosisfibrosisandinflammationinpatientswithnonalcoholicfattyliverdiseaseincomparisontoaliverbiopsy AT vukojaivan accuracyofserumbiomarkersinthediagnosisofsteatosisfibrosisandinflammationinpatientswithnonalcoholicfattyliverdiseaseincomparisontoaliverbiopsy AT puzpetra accuracyofserumbiomarkersinthediagnosisofsteatosisfibrosisandinflammationinpatientswithnonalcoholicfattyliverdiseaseincomparisontoaliverbiopsy AT aralicamerica accuracyofserumbiomarkersinthediagnosisofsteatosisfibrosisandinflammationinpatientswithnonalcoholicfattyliverdiseaseincomparisontoaliverbiopsy AT hausergoran accuracyofserumbiomarkersinthediagnosisofsteatosisfibrosisandinflammationinpatientswithnonalcoholicfattyliverdiseaseincomparisontoaliverbiopsy |