Material Properties and Morphology of Prestomal Teeth in Relation to the Feeding Habits of Diptera (Brachycera)

SIMPLE SUMMARY: The prestomal teeth are structures on the mouthparts of some fly species that rasp surfaces to expose liquids for ingestion. Here, we investigated the material properties of prestomal teeth, including their hardness, elastic modulus, the extent of sclerotization, and elemental composition, and combined these results with morphology to determine their relationship to fly feeding habits. The results indicated that the prestomal teeth are heavily sclerotized and have hardness and elastic modulus values similar to the polymer polycarbonate. Although the presence of inorganic elements contributes to a harder cuticle in other insect species, the fly species studied here had only low concentrations of inorganic elements. We found evidence that the material properties and morphology of prestomal teeth relate to feeding habits, not phylogeny. In particular, flies that pierce tissues for liquids have larger prestomal teeth relative to their mouthpart sizes when compared to species that generally feed on exposed liquids. Flies are one of the most successful groups of organisms and their success likely relates to their ability to feed on a large array of nutritional liquids. Given their importance in ecology systems and relevance to medical and veterinary entomology, the functional morphology of fly mouthparts warrants additional studies. ABSTRACT: Prestomal teeth are cuticular projections on the mouthparts of some fly species that rasp surfaces when feeding. Although prestomal teeth morphology has been reported for several fly species, their material properties have not been investigated. Here we report the morphology, elemental composition, extent of sclerotization, hardness, and elastic modulus of prestomal teeth and relate these findings to feeding habits. Scanning electron microscopy revealed that species categorized as flower visitors have a large labellum with numerous pseudotracheae and lack prestomal teeth, generalist species have these same features but with prestomal teeth, and specialist species that feed on blood or other insects have a smaller labellum with few or no pseudotracheae and relatively large prestomal teeth. Confocal microscopy revealed that prestomal teeth are heavily sclerotized and the labellum contains resilin, an elastomeric protein. Hardness and elastic modulus were explored with nanoindentation and showed that the insectivorous Scathophaga stercoraria had the hardest prestomal teeth and the highest modulus. Energy dispersive x-ray spectroscopy revealed that prestomal teeth had low concentrations of inorganic elements, suggesting that hardness might be partially supplemented by inorganic elements. Our findings indicate that prestomal teeth morphology and material properties relate more to feeding habits than to phylogeny.