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Implementation of an Analytical Method for Spectrophotometric Evaluation of Total Phenolic Content in Essential Oils

Over the past decade, there has been growing interest in polyphenols’ research since these compounds, as antioxidants, have several health benefits, such as preventing neurodegenerative diseases, inflammation, cancer, cardiovascular diseases, and type 2 diabetes. This study implements an analytical...

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Autores principales: Michiu, Delia, Socaciu, Maria-Ioana, Fogarasi, Melinda, Jimborean, Anamaria Mirela, Ranga, Floricuţa, Mureşan, Vlad, Semeniuc, Cristina Anamaria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8880325/
https://www.ncbi.nlm.nih.gov/pubmed/35209133
http://dx.doi.org/10.3390/molecules27041345
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author Michiu, Delia
Socaciu, Maria-Ioana
Fogarasi, Melinda
Jimborean, Anamaria Mirela
Ranga, Floricuţa
Mureşan, Vlad
Semeniuc, Cristina Anamaria
author_facet Michiu, Delia
Socaciu, Maria-Ioana
Fogarasi, Melinda
Jimborean, Anamaria Mirela
Ranga, Floricuţa
Mureşan, Vlad
Semeniuc, Cristina Anamaria
author_sort Michiu, Delia
collection PubMed
description Over the past decade, there has been growing interest in polyphenols’ research since these compounds, as antioxidants, have several health benefits, such as preventing neurodegenerative diseases, inflammation, cancer, cardiovascular diseases, and type 2 diabetes. This study implements an analytical method to assess the total phenolic content (TPC) in essential oils using Folin–Ciocalteu’s phenol reagent and quantifies the individual phenolic compounds by liquid chromatography. Thus, the research design and methodology included: (1) extraction of essential oil from dried thyme leaves by hydrodistillation; (2) spectrophotometric measurement of TPC by Folin–Ciocalteu method; and (3) identification and quantification of individual phenolic compounds by high-performance liquid chromatography-diode array detection/electrospray ionization mass spectrometry (HPLC-DAD-ESI-MS). Results revealed a TPC of 22.62 ± 0.482 mg GAE/100 µL and a polyphenolic profile characterized by phenolic acids (52.1%), flavonoids (16.1%), and other polyphenols (31.8%). Thymol, salvianolic acid A, and rosmarinic acid were the major compounds of thyme essential oil. The proposed analytical procedure has an acceptable level of repeatability, reproducibility, linearity, LOD (limit of detection), and LOQ (limit of quantification).
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spelling pubmed-88803252022-02-26 Implementation of an Analytical Method for Spectrophotometric Evaluation of Total Phenolic Content in Essential Oils Michiu, Delia Socaciu, Maria-Ioana Fogarasi, Melinda Jimborean, Anamaria Mirela Ranga, Floricuţa Mureşan, Vlad Semeniuc, Cristina Anamaria Molecules Article Over the past decade, there has been growing interest in polyphenols’ research since these compounds, as antioxidants, have several health benefits, such as preventing neurodegenerative diseases, inflammation, cancer, cardiovascular diseases, and type 2 diabetes. This study implements an analytical method to assess the total phenolic content (TPC) in essential oils using Folin–Ciocalteu’s phenol reagent and quantifies the individual phenolic compounds by liquid chromatography. Thus, the research design and methodology included: (1) extraction of essential oil from dried thyme leaves by hydrodistillation; (2) spectrophotometric measurement of TPC by Folin–Ciocalteu method; and (3) identification and quantification of individual phenolic compounds by high-performance liquid chromatography-diode array detection/electrospray ionization mass spectrometry (HPLC-DAD-ESI-MS). Results revealed a TPC of 22.62 ± 0.482 mg GAE/100 µL and a polyphenolic profile characterized by phenolic acids (52.1%), flavonoids (16.1%), and other polyphenols (31.8%). Thymol, salvianolic acid A, and rosmarinic acid were the major compounds of thyme essential oil. The proposed analytical procedure has an acceptable level of repeatability, reproducibility, linearity, LOD (limit of detection), and LOQ (limit of quantification). MDPI 2022-02-16 /pmc/articles/PMC8880325/ /pubmed/35209133 http://dx.doi.org/10.3390/molecules27041345 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Michiu, Delia
Socaciu, Maria-Ioana
Fogarasi, Melinda
Jimborean, Anamaria Mirela
Ranga, Floricuţa
Mureşan, Vlad
Semeniuc, Cristina Anamaria
Implementation of an Analytical Method for Spectrophotometric Evaluation of Total Phenolic Content in Essential Oils
title Implementation of an Analytical Method for Spectrophotometric Evaluation of Total Phenolic Content in Essential Oils
title_full Implementation of an Analytical Method for Spectrophotometric Evaluation of Total Phenolic Content in Essential Oils
title_fullStr Implementation of an Analytical Method for Spectrophotometric Evaluation of Total Phenolic Content in Essential Oils
title_full_unstemmed Implementation of an Analytical Method for Spectrophotometric Evaluation of Total Phenolic Content in Essential Oils
title_short Implementation of an Analytical Method for Spectrophotometric Evaluation of Total Phenolic Content in Essential Oils
title_sort implementation of an analytical method for spectrophotometric evaluation of total phenolic content in essential oils
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8880325/
https://www.ncbi.nlm.nih.gov/pubmed/35209133
http://dx.doi.org/10.3390/molecules27041345
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