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A Combination of Aqueous Extraction and Ultrafiltration for the Purification of Phycocyanin from Arthrospira maxima
The purification of phycocyanin (PC) from Spirulina generally involves a combination of different techniques. Here, we report the results on PC yields from a combined aqueous extraction-ultrafiltration (UF) process of a strain of Arthrospira maxima cultivated in a farm devoted to producing PC with f...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8880360/ https://www.ncbi.nlm.nih.gov/pubmed/35208763 http://dx.doi.org/10.3390/microorganisms10020308 |
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author | Nisticò, Dante Matteo Piro, Amalia Oliva, Daniela Osso, Vincenzo Mazzuca, Silvia Fagà, Francesco Antonio Morelli, Rosanna Conidi, Carmela Figoli, Alberto Cassano, Alfredo |
author_facet | Nisticò, Dante Matteo Piro, Amalia Oliva, Daniela Osso, Vincenzo Mazzuca, Silvia Fagà, Francesco Antonio Morelli, Rosanna Conidi, Carmela Figoli, Alberto Cassano, Alfredo |
author_sort | Nisticò, Dante Matteo |
collection | PubMed |
description | The purification of phycocyanin (PC) from Spirulina generally involves a combination of different techniques. Here, we report the results on PC yields from a combined aqueous extraction-ultrafiltration (UF) process of a strain of Arthrospira maxima cultivated in a farm devoted to producing PC with food-grade purity. Samples optimized from different biomass/solvent ratios were purified by using a polyethersulphone (PES) membrane with a molecular weight cut-off (MWCO) of 20 kDa. The UF system was operated at 2.0 ± 0.1 bar and at 24 ± 2 °C up to a volume concentration factor (VCF) of 5. A diafiltration (DF) process was conducted after UF in order to increase the PC recovery in the retentate. Samples were collected during both UF and DF processes in order to evaluate membrane productivity and PC purity. The average permeate fluxes of about 14.4 L/m(2)h were measured in the selected operating conditions and more than 96% of PC was rejected by the UF membrane independently ofthe extraction yields and times. The concentration of PC in the final retentate was 1.17 mg/mL; this confirmed the observed rejection and the final VCF of the process (about 5-fold when compared to the concentration of PC in the crude extract). In addition, the combination of UF and diafiltration allowed the removal of about 91.7% of the DNA from the crude extract, thereby improving the purity of the phycocyanin in the retentate fraction. |
format | Online Article Text |
id | pubmed-8880360 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88803602022-02-26 A Combination of Aqueous Extraction and Ultrafiltration for the Purification of Phycocyanin from Arthrospira maxima Nisticò, Dante Matteo Piro, Amalia Oliva, Daniela Osso, Vincenzo Mazzuca, Silvia Fagà, Francesco Antonio Morelli, Rosanna Conidi, Carmela Figoli, Alberto Cassano, Alfredo Microorganisms Article The purification of phycocyanin (PC) from Spirulina generally involves a combination of different techniques. Here, we report the results on PC yields from a combined aqueous extraction-ultrafiltration (UF) process of a strain of Arthrospira maxima cultivated in a farm devoted to producing PC with food-grade purity. Samples optimized from different biomass/solvent ratios were purified by using a polyethersulphone (PES) membrane with a molecular weight cut-off (MWCO) of 20 kDa. The UF system was operated at 2.0 ± 0.1 bar and at 24 ± 2 °C up to a volume concentration factor (VCF) of 5. A diafiltration (DF) process was conducted after UF in order to increase the PC recovery in the retentate. Samples were collected during both UF and DF processes in order to evaluate membrane productivity and PC purity. The average permeate fluxes of about 14.4 L/m(2)h were measured in the selected operating conditions and more than 96% of PC was rejected by the UF membrane independently ofthe extraction yields and times. The concentration of PC in the final retentate was 1.17 mg/mL; this confirmed the observed rejection and the final VCF of the process (about 5-fold when compared to the concentration of PC in the crude extract). In addition, the combination of UF and diafiltration allowed the removal of about 91.7% of the DNA from the crude extract, thereby improving the purity of the phycocyanin in the retentate fraction. MDPI 2022-01-28 /pmc/articles/PMC8880360/ /pubmed/35208763 http://dx.doi.org/10.3390/microorganisms10020308 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nisticò, Dante Matteo Piro, Amalia Oliva, Daniela Osso, Vincenzo Mazzuca, Silvia Fagà, Francesco Antonio Morelli, Rosanna Conidi, Carmela Figoli, Alberto Cassano, Alfredo A Combination of Aqueous Extraction and Ultrafiltration for the Purification of Phycocyanin from Arthrospira maxima |
title | A Combination of Aqueous Extraction and Ultrafiltration for the Purification of Phycocyanin from Arthrospira maxima |
title_full | A Combination of Aqueous Extraction and Ultrafiltration for the Purification of Phycocyanin from Arthrospira maxima |
title_fullStr | A Combination of Aqueous Extraction and Ultrafiltration for the Purification of Phycocyanin from Arthrospira maxima |
title_full_unstemmed | A Combination of Aqueous Extraction and Ultrafiltration for the Purification of Phycocyanin from Arthrospira maxima |
title_short | A Combination of Aqueous Extraction and Ultrafiltration for the Purification of Phycocyanin from Arthrospira maxima |
title_sort | combination of aqueous extraction and ultrafiltration for the purification of phycocyanin from arthrospira maxima |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8880360/ https://www.ncbi.nlm.nih.gov/pubmed/35208763 http://dx.doi.org/10.3390/microorganisms10020308 |
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