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Development and Evaluation of a Combined Contagious Bovine Pleuropneumonia (CBPP) and Lumpy Skin Disease (LSD) Live Vaccine

Mycoplasma mycoides subsp. mycoides (Mmm) is the causative agent of contagious bovine pleuropneumonia (CBPP). Lumpy skin disease (LSD) is a viral disease of cattle caused by lumpy skin disease virus (LSDV). LSD and CBPP are both transboundary diseases spreading in the same areas of Africa and Asia....

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Autores principales: Safini, Najete, Elmejdoub, Soufiane, Bamouh, Zahra, Jazouli, Mohamed, Hamdi, Jihane, Boumart, Zineb, Rhazi, Halima, Tadlaoui, Khalid Omari, El Harrak, Mehdi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8880402/
https://www.ncbi.nlm.nih.gov/pubmed/35215965
http://dx.doi.org/10.3390/v14020372
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author Safini, Najete
Elmejdoub, Soufiane
Bamouh, Zahra
Jazouli, Mohamed
Hamdi, Jihane
Boumart, Zineb
Rhazi, Halima
Tadlaoui, Khalid Omari
El Harrak, Mehdi
author_facet Safini, Najete
Elmejdoub, Soufiane
Bamouh, Zahra
Jazouli, Mohamed
Hamdi, Jihane
Boumart, Zineb
Rhazi, Halima
Tadlaoui, Khalid Omari
El Harrak, Mehdi
author_sort Safini, Najete
collection PubMed
description Mycoplasma mycoides subsp. mycoides (Mmm) is the causative agent of contagious bovine pleuropneumonia (CBPP). Lumpy skin disease (LSD) is a viral disease of cattle caused by lumpy skin disease virus (LSDV). LSD and CBPP are both transboundary diseases spreading in the same areas of Africa and Asia. A combination vaccine to control CBPP and LSD offers significant value to small-scale livestock keepers as a single administration. Access to a bivalent vaccine may improve vaccination rates for both pathogens. In the present study, we evaluated the LSDV/CBPP live combined vaccine by testing the generation of virus neutralizing antibodies, immunogenicity, and safety on target species. In-vitro assessment of the Mycoplasma effect on LSDV growth in cell culture was evaluated by infectious virus titration and qPCR during 3 serial passages, whereas in-vivo interference was assessed through the antibody response to vaccination. This combined Mmm/LSDV vaccine could be used to protect cattle against both diseases with a single vaccination in the endemic countries. There were no adverse reactions detected in this study and inoculated cattle produced high levels of specific antibodies starting from day 7 post-vaccination, suggesting that this combination vaccine is both safe and effective.
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spelling pubmed-88804022022-02-26 Development and Evaluation of a Combined Contagious Bovine Pleuropneumonia (CBPP) and Lumpy Skin Disease (LSD) Live Vaccine Safini, Najete Elmejdoub, Soufiane Bamouh, Zahra Jazouli, Mohamed Hamdi, Jihane Boumart, Zineb Rhazi, Halima Tadlaoui, Khalid Omari El Harrak, Mehdi Viruses Article Mycoplasma mycoides subsp. mycoides (Mmm) is the causative agent of contagious bovine pleuropneumonia (CBPP). Lumpy skin disease (LSD) is a viral disease of cattle caused by lumpy skin disease virus (LSDV). LSD and CBPP are both transboundary diseases spreading in the same areas of Africa and Asia. A combination vaccine to control CBPP and LSD offers significant value to small-scale livestock keepers as a single administration. Access to a bivalent vaccine may improve vaccination rates for both pathogens. In the present study, we evaluated the LSDV/CBPP live combined vaccine by testing the generation of virus neutralizing antibodies, immunogenicity, and safety on target species. In-vitro assessment of the Mycoplasma effect on LSDV growth in cell culture was evaluated by infectious virus titration and qPCR during 3 serial passages, whereas in-vivo interference was assessed through the antibody response to vaccination. This combined Mmm/LSDV vaccine could be used to protect cattle against both diseases with a single vaccination in the endemic countries. There were no adverse reactions detected in this study and inoculated cattle produced high levels of specific antibodies starting from day 7 post-vaccination, suggesting that this combination vaccine is both safe and effective. MDPI 2022-02-11 /pmc/articles/PMC8880402/ /pubmed/35215965 http://dx.doi.org/10.3390/v14020372 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Safini, Najete
Elmejdoub, Soufiane
Bamouh, Zahra
Jazouli, Mohamed
Hamdi, Jihane
Boumart, Zineb
Rhazi, Halima
Tadlaoui, Khalid Omari
El Harrak, Mehdi
Development and Evaluation of a Combined Contagious Bovine Pleuropneumonia (CBPP) and Lumpy Skin Disease (LSD) Live Vaccine
title Development and Evaluation of a Combined Contagious Bovine Pleuropneumonia (CBPP) and Lumpy Skin Disease (LSD) Live Vaccine
title_full Development and Evaluation of a Combined Contagious Bovine Pleuropneumonia (CBPP) and Lumpy Skin Disease (LSD) Live Vaccine
title_fullStr Development and Evaluation of a Combined Contagious Bovine Pleuropneumonia (CBPP) and Lumpy Skin Disease (LSD) Live Vaccine
title_full_unstemmed Development and Evaluation of a Combined Contagious Bovine Pleuropneumonia (CBPP) and Lumpy Skin Disease (LSD) Live Vaccine
title_short Development and Evaluation of a Combined Contagious Bovine Pleuropneumonia (CBPP) and Lumpy Skin Disease (LSD) Live Vaccine
title_sort development and evaluation of a combined contagious bovine pleuropneumonia (cbpp) and lumpy skin disease (lsd) live vaccine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8880402/
https://www.ncbi.nlm.nih.gov/pubmed/35215965
http://dx.doi.org/10.3390/v14020372
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