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Molecular Epidemiology of Norovirus (NoV) Infection in Mie Prefecture: The Kinetics of Norovirus Antigenemia in Pediatric Patients

Few studies have shown the presence of norovirus (NoV) RNA in blood circulation but there is no data on norovirus antigenemia. We examined both antigenemia and RNAemia from the sera of children with NoV infections and studied whether norovirus antigenemia is correlated with the levels of norovirus-s...

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Autores principales: Amexo, Jennifer X., Negoro, Manami, Kuurdor, Elijah Deku-Mwin, Lartey, Belinda L., Sokejima, Shigeru, Sugata, Ken, Tonto, Prince Baffour, Taniguchi, Kiyosu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8880472/
https://www.ncbi.nlm.nih.gov/pubmed/35215766
http://dx.doi.org/10.3390/v14020173
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author Amexo, Jennifer X.
Negoro, Manami
Kuurdor, Elijah Deku-Mwin
Lartey, Belinda L.
Sokejima, Shigeru
Sugata, Ken
Tonto, Prince Baffour
Taniguchi, Kiyosu
author_facet Amexo, Jennifer X.
Negoro, Manami
Kuurdor, Elijah Deku-Mwin
Lartey, Belinda L.
Sokejima, Shigeru
Sugata, Ken
Tonto, Prince Baffour
Taniguchi, Kiyosu
author_sort Amexo, Jennifer X.
collection PubMed
description Few studies have shown the presence of norovirus (NoV) RNA in blood circulation but there is no data on norovirus antigenemia. We examined both antigenemia and RNAemia from the sera of children with NoV infections and studied whether norovirus antigenemia is correlated with the levels of norovirus-specific antibodies and clinical severity of gastroenteritis. Both stool and serum samples were collected from 63 children admitted to Mie National Hospital with acute NoV gastroenteritis. Norovirus antigen and RNA were detected in sera by ELISA and real-time RT-PCR, respectively. NoV antigenemia was found in 54.8% (34/62) and RNAemia in 14.3% (9/63) of sera samples. Antigenemia was more common in the younger age group (0–2 years) than in the older age groups, and most patients were male. There was no correlation between stool viral load and norovirus antigen (NoV-Ag) levels (r(s) = −0.063; Cl −0.3150 to 0.1967; p = 0.6251). Higher levels of acute norovirus-specific IgG serum antibodies resulted in a lower antigenemia OD value (n = 61; r = −0.4258; CI −0.62 to −0.19; p = 0.0006). Norovirus antigenemia occurred more commonly in children under 2 years of age with NoV-associated acute gastroenteritis. The occurrence of antigenemia was not correlated with stool viral load or disease severity.
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spelling pubmed-88804722022-02-26 Molecular Epidemiology of Norovirus (NoV) Infection in Mie Prefecture: The Kinetics of Norovirus Antigenemia in Pediatric Patients Amexo, Jennifer X. Negoro, Manami Kuurdor, Elijah Deku-Mwin Lartey, Belinda L. Sokejima, Shigeru Sugata, Ken Tonto, Prince Baffour Taniguchi, Kiyosu Viruses Article Few studies have shown the presence of norovirus (NoV) RNA in blood circulation but there is no data on norovirus antigenemia. We examined both antigenemia and RNAemia from the sera of children with NoV infections and studied whether norovirus antigenemia is correlated with the levels of norovirus-specific antibodies and clinical severity of gastroenteritis. Both stool and serum samples were collected from 63 children admitted to Mie National Hospital with acute NoV gastroenteritis. Norovirus antigen and RNA were detected in sera by ELISA and real-time RT-PCR, respectively. NoV antigenemia was found in 54.8% (34/62) and RNAemia in 14.3% (9/63) of sera samples. Antigenemia was more common in the younger age group (0–2 years) than in the older age groups, and most patients were male. There was no correlation between stool viral load and norovirus antigen (NoV-Ag) levels (r(s) = −0.063; Cl −0.3150 to 0.1967; p = 0.6251). Higher levels of acute norovirus-specific IgG serum antibodies resulted in a lower antigenemia OD value (n = 61; r = −0.4258; CI −0.62 to −0.19; p = 0.0006). Norovirus antigenemia occurred more commonly in children under 2 years of age with NoV-associated acute gastroenteritis. The occurrence of antigenemia was not correlated with stool viral load or disease severity. MDPI 2022-01-18 /pmc/articles/PMC8880472/ /pubmed/35215766 http://dx.doi.org/10.3390/v14020173 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Amexo, Jennifer X.
Negoro, Manami
Kuurdor, Elijah Deku-Mwin
Lartey, Belinda L.
Sokejima, Shigeru
Sugata, Ken
Tonto, Prince Baffour
Taniguchi, Kiyosu
Molecular Epidemiology of Norovirus (NoV) Infection in Mie Prefecture: The Kinetics of Norovirus Antigenemia in Pediatric Patients
title Molecular Epidemiology of Norovirus (NoV) Infection in Mie Prefecture: The Kinetics of Norovirus Antigenemia in Pediatric Patients
title_full Molecular Epidemiology of Norovirus (NoV) Infection in Mie Prefecture: The Kinetics of Norovirus Antigenemia in Pediatric Patients
title_fullStr Molecular Epidemiology of Norovirus (NoV) Infection in Mie Prefecture: The Kinetics of Norovirus Antigenemia in Pediatric Patients
title_full_unstemmed Molecular Epidemiology of Norovirus (NoV) Infection in Mie Prefecture: The Kinetics of Norovirus Antigenemia in Pediatric Patients
title_short Molecular Epidemiology of Norovirus (NoV) Infection in Mie Prefecture: The Kinetics of Norovirus Antigenemia in Pediatric Patients
title_sort molecular epidemiology of norovirus (nov) infection in mie prefecture: the kinetics of norovirus antigenemia in pediatric patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8880472/
https://www.ncbi.nlm.nih.gov/pubmed/35215766
http://dx.doi.org/10.3390/v14020173
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