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Application of Optical Methods for Determination of Concentration of Doxorubicin in Blood and Plasma
The aim of presented research is to develop a simple and quick method of spectrophotometric detection for the determination of doxorubicin hydrochloride in blood and plasma. Anthracycline antibiotics are among the most effective antineoplastic agents. However, despite their high efficacy in the trea...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8880482/ https://www.ncbi.nlm.nih.gov/pubmed/35215225 http://dx.doi.org/10.3390/ph15020112 |
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author | Sikora, Tomasz Morawska, Karolina Lisowski, Wiesław Rytel, Paweł Dylong, Agnieszka |
author_facet | Sikora, Tomasz Morawska, Karolina Lisowski, Wiesław Rytel, Paweł Dylong, Agnieszka |
author_sort | Sikora, Tomasz |
collection | PubMed |
description | The aim of presented research is to develop a simple and quick method of spectrophotometric detection for the determination of doxorubicin hydrochloride in blood and plasma. Anthracycline antibiotics are among the most effective antineoplastic agents. However, despite their high efficacy in the treatment of various types of cancer, their administration is limited primarily because they exhibit myocardial toxicity. This may be a limiting factor in the dosage of medications; nevertheless, drugs exhibiting this mechanism of action constitute a very important group of chemotherapeutics. One of the more widely studied antibiotics from the anthracycline group is doxorubicin. It exhibits the highest antineoplastic activity from among a number of derivative compounds. Because of the adverse effects of doxorubicin, especially cardiotoxicity, it is important to maintain control of its concentration in body fluids. The method in the study consists of extraction doxorubicin from the plasma or blood and measurements of the absorbance of light in the visible light range in a DOX solution with respect to a reference sample. The research used blood and plasma samples spiked with doxorubicin to give concentrations in the range of 0.2–10 µg/mL. Obtained LODs were 1.6 µg/mL and 1.2 µg/mL, respectively. |
format | Online Article Text |
id | pubmed-8880482 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88804822022-02-26 Application of Optical Methods for Determination of Concentration of Doxorubicin in Blood and Plasma Sikora, Tomasz Morawska, Karolina Lisowski, Wiesław Rytel, Paweł Dylong, Agnieszka Pharmaceuticals (Basel) Article The aim of presented research is to develop a simple and quick method of spectrophotometric detection for the determination of doxorubicin hydrochloride in blood and plasma. Anthracycline antibiotics are among the most effective antineoplastic agents. However, despite their high efficacy in the treatment of various types of cancer, their administration is limited primarily because they exhibit myocardial toxicity. This may be a limiting factor in the dosage of medications; nevertheless, drugs exhibiting this mechanism of action constitute a very important group of chemotherapeutics. One of the more widely studied antibiotics from the anthracycline group is doxorubicin. It exhibits the highest antineoplastic activity from among a number of derivative compounds. Because of the adverse effects of doxorubicin, especially cardiotoxicity, it is important to maintain control of its concentration in body fluids. The method in the study consists of extraction doxorubicin from the plasma or blood and measurements of the absorbance of light in the visible light range in a DOX solution with respect to a reference sample. The research used blood and plasma samples spiked with doxorubicin to give concentrations in the range of 0.2–10 µg/mL. Obtained LODs were 1.6 µg/mL and 1.2 µg/mL, respectively. MDPI 2022-01-18 /pmc/articles/PMC8880482/ /pubmed/35215225 http://dx.doi.org/10.3390/ph15020112 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sikora, Tomasz Morawska, Karolina Lisowski, Wiesław Rytel, Paweł Dylong, Agnieszka Application of Optical Methods for Determination of Concentration of Doxorubicin in Blood and Plasma |
title | Application of Optical Methods for Determination of Concentration of Doxorubicin in Blood and Plasma |
title_full | Application of Optical Methods for Determination of Concentration of Doxorubicin in Blood and Plasma |
title_fullStr | Application of Optical Methods for Determination of Concentration of Doxorubicin in Blood and Plasma |
title_full_unstemmed | Application of Optical Methods for Determination of Concentration of Doxorubicin in Blood and Plasma |
title_short | Application of Optical Methods for Determination of Concentration of Doxorubicin in Blood and Plasma |
title_sort | application of optical methods for determination of concentration of doxorubicin in blood and plasma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8880482/ https://www.ncbi.nlm.nih.gov/pubmed/35215225 http://dx.doi.org/10.3390/ph15020112 |
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