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Identification of Antimycobacterial Natural Products from a Library of Marine Invertebrate Extracts
Tuberculosis (TB) remains a public health crisis, requiring the urgent identification of new anti-mycobacterial drugs. We screened several organic and aqueous marine invertebrate extracts for their in vitro inhibitory activity against the causative organism, Mycobacterium tuberculosis. Here, we repo...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8880526/ https://www.ncbi.nlm.nih.gov/pubmed/35200753 http://dx.doi.org/10.3390/medicines9020009 |
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author | Acquah, Kojo Sekyi Beukes, Denzil R. Seldon, Ronnett Jordaan, Audrey Sunassee, Suthananda N. Warner, Digby F. Gammon, David W. |
author_facet | Acquah, Kojo Sekyi Beukes, Denzil R. Seldon, Ronnett Jordaan, Audrey Sunassee, Suthananda N. Warner, Digby F. Gammon, David W. |
author_sort | Acquah, Kojo Sekyi |
collection | PubMed |
description | Tuberculosis (TB) remains a public health crisis, requiring the urgent identification of new anti-mycobacterial drugs. We screened several organic and aqueous marine invertebrate extracts for their in vitro inhibitory activity against the causative organism, Mycobacterium tuberculosis. Here, we report the results obtained for 54 marine invertebrate extracts. The chemical components of two of the extracts were dereplicated, using (1)H NMR and HR-LCMS with GNPS molecular networking, and these extracts were further subjected to an activity-guided isolation process to purify the bioactive components. Hyrtios reticulatus yielded heteronemin 1 and Jaspis splendens was found to produce the bengamide class of compounds, of which bengamides P 2 and Q 3 were isolated, while a new derivative, bengamide S 5, was putatively identified and its structure predicted, based on the similarity of its MS/MS fragmentation pattern to those of other bengamides. The isolated bioactive metabolites and semi-pure fractions exhibited M. tuberculosis growth inhibitory activity, in the range <0.24 to 62.50 µg/mL. This study establishes the bengamides as potent antitubercular compounds, with the first report of whole-cell antitubercular activity of bengamides P 2 and Q 3. |
format | Online Article Text |
id | pubmed-8880526 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-88805262022-02-26 Identification of Antimycobacterial Natural Products from a Library of Marine Invertebrate Extracts Acquah, Kojo Sekyi Beukes, Denzil R. Seldon, Ronnett Jordaan, Audrey Sunassee, Suthananda N. Warner, Digby F. Gammon, David W. Medicines (Basel) Article Tuberculosis (TB) remains a public health crisis, requiring the urgent identification of new anti-mycobacterial drugs. We screened several organic and aqueous marine invertebrate extracts for their in vitro inhibitory activity against the causative organism, Mycobacterium tuberculosis. Here, we report the results obtained for 54 marine invertebrate extracts. The chemical components of two of the extracts were dereplicated, using (1)H NMR and HR-LCMS with GNPS molecular networking, and these extracts were further subjected to an activity-guided isolation process to purify the bioactive components. Hyrtios reticulatus yielded heteronemin 1 and Jaspis splendens was found to produce the bengamide class of compounds, of which bengamides P 2 and Q 3 were isolated, while a new derivative, bengamide S 5, was putatively identified and its structure predicted, based on the similarity of its MS/MS fragmentation pattern to those of other bengamides. The isolated bioactive metabolites and semi-pure fractions exhibited M. tuberculosis growth inhibitory activity, in the range <0.24 to 62.50 µg/mL. This study establishes the bengamides as potent antitubercular compounds, with the first report of whole-cell antitubercular activity of bengamides P 2 and Q 3. MDPI 2022-01-28 /pmc/articles/PMC8880526/ /pubmed/35200753 http://dx.doi.org/10.3390/medicines9020009 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Acquah, Kojo Sekyi Beukes, Denzil R. Seldon, Ronnett Jordaan, Audrey Sunassee, Suthananda N. Warner, Digby F. Gammon, David W. Identification of Antimycobacterial Natural Products from a Library of Marine Invertebrate Extracts |
title | Identification of Antimycobacterial Natural Products from a Library of Marine Invertebrate Extracts |
title_full | Identification of Antimycobacterial Natural Products from a Library of Marine Invertebrate Extracts |
title_fullStr | Identification of Antimycobacterial Natural Products from a Library of Marine Invertebrate Extracts |
title_full_unstemmed | Identification of Antimycobacterial Natural Products from a Library of Marine Invertebrate Extracts |
title_short | Identification of Antimycobacterial Natural Products from a Library of Marine Invertebrate Extracts |
title_sort | identification of antimycobacterial natural products from a library of marine invertebrate extracts |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8880526/ https://www.ncbi.nlm.nih.gov/pubmed/35200753 http://dx.doi.org/10.3390/medicines9020009 |
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