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Neglected Diseases—Parasitic Infections among Slovakian Children from Different Populations and Genotypes of Giardia duodenalis

Children are most prone to parasitic infections. The objectives of the study were to examine the occurrence of parasitic infections in children from different populations and to perform molecular characterization of human Giardia duodenalis isolates. We examined 631 stool samples from Roma and non-R...

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Autores principales: Šmigová, Júlia, Šnábel, Viliam, Cavallero, Serena, Šmiga, Ľubomír, Šoltys, Jindřich, Papaj, Ján, Papajová, Ingrid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8880538/
https://www.ncbi.nlm.nih.gov/pubmed/35208836
http://dx.doi.org/10.3390/microorganisms10020381
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author Šmigová, Júlia
Šnábel, Viliam
Cavallero, Serena
Šmiga, Ľubomír
Šoltys, Jindřich
Papaj, Ján
Papajová, Ingrid
author_facet Šmigová, Júlia
Šnábel, Viliam
Cavallero, Serena
Šmiga, Ľubomír
Šoltys, Jindřich
Papaj, Ján
Papajová, Ingrid
author_sort Šmigová, Júlia
collection PubMed
description Children are most prone to parasitic infections. The objectives of the study were to examine the occurrence of parasitic infections in children from different populations and to perform molecular characterization of human Giardia duodenalis isolates. We examined 631 stool samples from Roma and non-Roma children for the presence of parasitic developmental stages. Samples were collected from three eastern Slovakia districts. The ages of the children ranged from 1 months to 17 years. Subsequently, the molecular characterization of human G. duodenalis isolates by PCR detected triosephosphate isomerase (tpi) and beta-giardin (bg) genes was performed. The overall prevalence of parasitic infection was 19.8%. Ascaris lumbricoides eggs were the most frequent, with an occurrence of about 13.8%. G. duodenalis cysts were present in 6.3% of samples. G. duodenalis isolates obtained from 13 children were subjected to DNA sequencing with tpi and bg genes. Five isolates were categorized as bearing subassemblage BIII, the three isolates as subassemblage BIV, one person was infected with a mixture of subassemblages BIII and BIV, four children had subassemblage AII, and one isolate revealed a structure corresponding with subassemblage AI. Our work is proof that poverty and poor hygiene contribute the most to public health problems associated with neglected parasitic diseases.
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spelling pubmed-88805382022-02-26 Neglected Diseases—Parasitic Infections among Slovakian Children from Different Populations and Genotypes of Giardia duodenalis Šmigová, Júlia Šnábel, Viliam Cavallero, Serena Šmiga, Ľubomír Šoltys, Jindřich Papaj, Ján Papajová, Ingrid Microorganisms Article Children are most prone to parasitic infections. The objectives of the study were to examine the occurrence of parasitic infections in children from different populations and to perform molecular characterization of human Giardia duodenalis isolates. We examined 631 stool samples from Roma and non-Roma children for the presence of parasitic developmental stages. Samples were collected from three eastern Slovakia districts. The ages of the children ranged from 1 months to 17 years. Subsequently, the molecular characterization of human G. duodenalis isolates by PCR detected triosephosphate isomerase (tpi) and beta-giardin (bg) genes was performed. The overall prevalence of parasitic infection was 19.8%. Ascaris lumbricoides eggs were the most frequent, with an occurrence of about 13.8%. G. duodenalis cysts were present in 6.3% of samples. G. duodenalis isolates obtained from 13 children were subjected to DNA sequencing with tpi and bg genes. Five isolates were categorized as bearing subassemblage BIII, the three isolates as subassemblage BIV, one person was infected with a mixture of subassemblages BIII and BIV, four children had subassemblage AII, and one isolate revealed a structure corresponding with subassemblage AI. Our work is proof that poverty and poor hygiene contribute the most to public health problems associated with neglected parasitic diseases. MDPI 2022-02-06 /pmc/articles/PMC8880538/ /pubmed/35208836 http://dx.doi.org/10.3390/microorganisms10020381 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Šmigová, Júlia
Šnábel, Viliam
Cavallero, Serena
Šmiga, Ľubomír
Šoltys, Jindřich
Papaj, Ján
Papajová, Ingrid
Neglected Diseases—Parasitic Infections among Slovakian Children from Different Populations and Genotypes of Giardia duodenalis
title Neglected Diseases—Parasitic Infections among Slovakian Children from Different Populations and Genotypes of Giardia duodenalis
title_full Neglected Diseases—Parasitic Infections among Slovakian Children from Different Populations and Genotypes of Giardia duodenalis
title_fullStr Neglected Diseases—Parasitic Infections among Slovakian Children from Different Populations and Genotypes of Giardia duodenalis
title_full_unstemmed Neglected Diseases—Parasitic Infections among Slovakian Children from Different Populations and Genotypes of Giardia duodenalis
title_short Neglected Diseases—Parasitic Infections among Slovakian Children from Different Populations and Genotypes of Giardia duodenalis
title_sort neglected diseases—parasitic infections among slovakian children from different populations and genotypes of giardia duodenalis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8880538/
https://www.ncbi.nlm.nih.gov/pubmed/35208836
http://dx.doi.org/10.3390/microorganisms10020381
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