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Risk factors for bovine rotavirus infection and genotyping of bovine rotavirus in diarrheic calves in Bangladesh

Bovine rotavirus (BRV) is considered the leading cause of calf diarrhea worldwide, including Bangladesh. In this study we aimed to identify risk factors for BRV infection and determine the G and P genotypes of BRV strains in diarrheic calves. Fecal samples were collected from 200 diarrheic calves in...

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Autores principales: Uddin Ahmed, Nasir, Khair, Abul, Hassan, Jayedul, Khan, Md. Abu Hadi Noor Ali, Rahman, A. K. M. Anisur, Hoque, Warda, Rahman, Mustafizur, Kobayashi, Nobumichi, Ward, Michael P., Alam, Md. Mahbub
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8880881/
https://www.ncbi.nlm.nih.gov/pubmed/35213667
http://dx.doi.org/10.1371/journal.pone.0264577
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author Uddin Ahmed, Nasir
Khair, Abul
Hassan, Jayedul
Khan, Md. Abu Hadi Noor Ali
Rahman, A. K. M. Anisur
Hoque, Warda
Rahman, Mustafizur
Kobayashi, Nobumichi
Ward, Michael P.
Alam, Md. Mahbub
author_facet Uddin Ahmed, Nasir
Khair, Abul
Hassan, Jayedul
Khan, Md. Abu Hadi Noor Ali
Rahman, A. K. M. Anisur
Hoque, Warda
Rahman, Mustafizur
Kobayashi, Nobumichi
Ward, Michael P.
Alam, Md. Mahbub
author_sort Uddin Ahmed, Nasir
collection PubMed
description Bovine rotavirus (BRV) is considered the leading cause of calf diarrhea worldwide, including Bangladesh. In this study we aimed to identify risk factors for BRV infection and determine the G and P genotypes of BRV strains in diarrheic calves. Fecal samples were collected from 200 diarrheic calves in three districts between January 2014 and October 2015. These samples were screened to detect the presence of BRV using rapid test-strips BIO K 152 (RTSBK). The RTSBK positive samples were further tested by polyacrylamide gel electrophoresis and the silver staining technique to detect rotavirus dsRNA. Risk factors were identified by multivariable logistic regression analysis. The G and P genotypes of BRV were determined by RT-PCR and sequencing. A phylogenetic tree was constructed based on the neighbor-joining method using CLC sequence viewer 8.0. About 23% of the diarrheic calves were BRV positive. The odds of BRV infection were 3.8- (95% confidence interval [95% CI]: 1.0–14.7) and 3.9-times (95% CI:1.1–14.2) higher in Barisal and Madaripur districts, respectively, than Sirrajganj. The risk of BRV infection was 3.1-times (95% CI: 1.5–6.5) higher in calves aged ≤ 5 weeks than those aged >5 weeks. Moreover, the risk of BRV infection was 2.6-times (95% CI:1.1–5.8) higher in crossbred (Holstein Friesian, Shahiwal) than indigenous calves. G6P[11] was the predominant genotype (94.4%), followed by G10P[11] (5.6%). The BRV G6 strains were found to be closest (98.9–99.9%) to Indian strains, and BRV G10 strains showed 99.9% identities with Indian strain. The VP4 gene of all P[11] strains showed >90% identities to each other and also with Indian strains. The most frequently identified BRV genotype was G6P[11]. About 23% of calf diarrhea cases were associated with BRV. To control disease, high-risk areas and younger crossbred calves should be targeted for surveillance and management. The predominant genotype could be utilized as the future vaccine candidate or vaccines with the dominant genotype should be used to control BRV diarrhea in Bangladesh.
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spelling pubmed-88808812022-02-26 Risk factors for bovine rotavirus infection and genotyping of bovine rotavirus in diarrheic calves in Bangladesh Uddin Ahmed, Nasir Khair, Abul Hassan, Jayedul Khan, Md. Abu Hadi Noor Ali Rahman, A. K. M. Anisur Hoque, Warda Rahman, Mustafizur Kobayashi, Nobumichi Ward, Michael P. Alam, Md. Mahbub PLoS One Research Article Bovine rotavirus (BRV) is considered the leading cause of calf diarrhea worldwide, including Bangladesh. In this study we aimed to identify risk factors for BRV infection and determine the G and P genotypes of BRV strains in diarrheic calves. Fecal samples were collected from 200 diarrheic calves in three districts between January 2014 and October 2015. These samples were screened to detect the presence of BRV using rapid test-strips BIO K 152 (RTSBK). The RTSBK positive samples were further tested by polyacrylamide gel electrophoresis and the silver staining technique to detect rotavirus dsRNA. Risk factors were identified by multivariable logistic regression analysis. The G and P genotypes of BRV were determined by RT-PCR and sequencing. A phylogenetic tree was constructed based on the neighbor-joining method using CLC sequence viewer 8.0. About 23% of the diarrheic calves were BRV positive. The odds of BRV infection were 3.8- (95% confidence interval [95% CI]: 1.0–14.7) and 3.9-times (95% CI:1.1–14.2) higher in Barisal and Madaripur districts, respectively, than Sirrajganj. The risk of BRV infection was 3.1-times (95% CI: 1.5–6.5) higher in calves aged ≤ 5 weeks than those aged >5 weeks. Moreover, the risk of BRV infection was 2.6-times (95% CI:1.1–5.8) higher in crossbred (Holstein Friesian, Shahiwal) than indigenous calves. G6P[11] was the predominant genotype (94.4%), followed by G10P[11] (5.6%). The BRV G6 strains were found to be closest (98.9–99.9%) to Indian strains, and BRV G10 strains showed 99.9% identities with Indian strain. The VP4 gene of all P[11] strains showed >90% identities to each other and also with Indian strains. The most frequently identified BRV genotype was G6P[11]. About 23% of calf diarrhea cases were associated with BRV. To control disease, high-risk areas and younger crossbred calves should be targeted for surveillance and management. The predominant genotype could be utilized as the future vaccine candidate or vaccines with the dominant genotype should be used to control BRV diarrhea in Bangladesh. Public Library of Science 2022-02-25 /pmc/articles/PMC8880881/ /pubmed/35213667 http://dx.doi.org/10.1371/journal.pone.0264577 Text en © 2022 Uddin Ahmed et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Uddin Ahmed, Nasir
Khair, Abul
Hassan, Jayedul
Khan, Md. Abu Hadi Noor Ali
Rahman, A. K. M. Anisur
Hoque, Warda
Rahman, Mustafizur
Kobayashi, Nobumichi
Ward, Michael P.
Alam, Md. Mahbub
Risk factors for bovine rotavirus infection and genotyping of bovine rotavirus in diarrheic calves in Bangladesh
title Risk factors for bovine rotavirus infection and genotyping of bovine rotavirus in diarrheic calves in Bangladesh
title_full Risk factors for bovine rotavirus infection and genotyping of bovine rotavirus in diarrheic calves in Bangladesh
title_fullStr Risk factors for bovine rotavirus infection and genotyping of bovine rotavirus in diarrheic calves in Bangladesh
title_full_unstemmed Risk factors for bovine rotavirus infection and genotyping of bovine rotavirus in diarrheic calves in Bangladesh
title_short Risk factors for bovine rotavirus infection and genotyping of bovine rotavirus in diarrheic calves in Bangladesh
title_sort risk factors for bovine rotavirus infection and genotyping of bovine rotavirus in diarrheic calves in bangladesh
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8880881/
https://www.ncbi.nlm.nih.gov/pubmed/35213667
http://dx.doi.org/10.1371/journal.pone.0264577
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