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Changes in Skeletal Muscle PAK1 Levels Regulate Tissue Crosstalk to Impact Whole Body Glucose Homeostasis

Skeletal muscle accounts for ~80% of insulin-stimulated glucose uptake. The Group I p21–activated kinase 1 (PAK1) is required for the non-canonical insulin-stimulated GLUT4 vesicle translocation in skeletal muscle cells. We found that the abundances of PAK1 protein and its downstream effector in mus...

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Autores principales: Merz, Karla E., Tunduguru, Ragadeepthi, Ahn, Miwon, Salunkhe, Vishal A., Veluthakal, Rajakrishnan, Hwang, Jinhee, Bhattacharya, Supriyo, McCown, Erika M., Garcia, Pablo A., Zhou, Chunxue, Oh, Eunjin, Yoder, Stephanie M., Elmendorf, Jeffrey S., Thurmond, Debbie C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8881144/
https://www.ncbi.nlm.nih.gov/pubmed/35222279
http://dx.doi.org/10.3389/fendo.2022.821849
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author Merz, Karla E.
Tunduguru, Ragadeepthi
Ahn, Miwon
Salunkhe, Vishal A.
Veluthakal, Rajakrishnan
Hwang, Jinhee
Bhattacharya, Supriyo
McCown, Erika M.
Garcia, Pablo A.
Zhou, Chunxue
Oh, Eunjin
Yoder, Stephanie M.
Elmendorf, Jeffrey S.
Thurmond, Debbie C.
author_facet Merz, Karla E.
Tunduguru, Ragadeepthi
Ahn, Miwon
Salunkhe, Vishal A.
Veluthakal, Rajakrishnan
Hwang, Jinhee
Bhattacharya, Supriyo
McCown, Erika M.
Garcia, Pablo A.
Zhou, Chunxue
Oh, Eunjin
Yoder, Stephanie M.
Elmendorf, Jeffrey S.
Thurmond, Debbie C.
author_sort Merz, Karla E.
collection PubMed
description Skeletal muscle accounts for ~80% of insulin-stimulated glucose uptake. The Group I p21–activated kinase 1 (PAK1) is required for the non-canonical insulin-stimulated GLUT4 vesicle translocation in skeletal muscle cells. We found that the abundances of PAK1 protein and its downstream effector in muscle, ARPC1B, are significantly reduced in the skeletal muscle of humans with type 2 diabetes, compared to the non-diabetic controls, making skeletal muscle PAK1 a candidate regulator of glucose homeostasis. Although whole-body PAK1 knockout mice exhibit glucose intolerance and are insulin resistant, the contribution of skeletal muscle PAK1 in particular was unknown. As such, we developed inducible skeletal muscle-specific PAK1 knockout (skmPAK1-iKO) and overexpression (skmPAK1-iOE) mouse models to evaluate the role of PAK1 in skeletal muscle insulin sensitivity and glucose homeostasis. Using intraperitoneal glucose tolerance and insulin tolerance testing, we found that skeletal muscle PAK1 is required for maintaining whole body glucose homeostasis. Moreover, PAK1 enrichment in GLUT4-myc-L6 myoblasts preserves normal insulin-stimulated GLUT4 translocation under insulin resistance conditions. Unexpectedly, skmPAK1-iKO also showed aberrant plasma insulin levels following a glucose challenge. By applying conditioned media from PAK1-enriched myotubes or myoblasts to β-cells in culture, we established that a muscle-derived circulating factor(s) could enhance β-cell function. Taken together, these data suggest that PAK1 levels in the skeletal muscle can regulate not only skeletal muscle insulin sensitivity, but can also engage in tissue crosstalk with pancreatic β-cells, unveiling a new molecular mechanism by which PAK1 regulates whole-body glucose homeostasis.
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spelling pubmed-88811442022-02-26 Changes in Skeletal Muscle PAK1 Levels Regulate Tissue Crosstalk to Impact Whole Body Glucose Homeostasis Merz, Karla E. Tunduguru, Ragadeepthi Ahn, Miwon Salunkhe, Vishal A. Veluthakal, Rajakrishnan Hwang, Jinhee Bhattacharya, Supriyo McCown, Erika M. Garcia, Pablo A. Zhou, Chunxue Oh, Eunjin Yoder, Stephanie M. Elmendorf, Jeffrey S. Thurmond, Debbie C. Front Endocrinol (Lausanne) Endocrinology Skeletal muscle accounts for ~80% of insulin-stimulated glucose uptake. The Group I p21–activated kinase 1 (PAK1) is required for the non-canonical insulin-stimulated GLUT4 vesicle translocation in skeletal muscle cells. We found that the abundances of PAK1 protein and its downstream effector in muscle, ARPC1B, are significantly reduced in the skeletal muscle of humans with type 2 diabetes, compared to the non-diabetic controls, making skeletal muscle PAK1 a candidate regulator of glucose homeostasis. Although whole-body PAK1 knockout mice exhibit glucose intolerance and are insulin resistant, the contribution of skeletal muscle PAK1 in particular was unknown. As such, we developed inducible skeletal muscle-specific PAK1 knockout (skmPAK1-iKO) and overexpression (skmPAK1-iOE) mouse models to evaluate the role of PAK1 in skeletal muscle insulin sensitivity and glucose homeostasis. Using intraperitoneal glucose tolerance and insulin tolerance testing, we found that skeletal muscle PAK1 is required for maintaining whole body glucose homeostasis. Moreover, PAK1 enrichment in GLUT4-myc-L6 myoblasts preserves normal insulin-stimulated GLUT4 translocation under insulin resistance conditions. Unexpectedly, skmPAK1-iKO also showed aberrant plasma insulin levels following a glucose challenge. By applying conditioned media from PAK1-enriched myotubes or myoblasts to β-cells in culture, we established that a muscle-derived circulating factor(s) could enhance β-cell function. Taken together, these data suggest that PAK1 levels in the skeletal muscle can regulate not only skeletal muscle insulin sensitivity, but can also engage in tissue crosstalk with pancreatic β-cells, unveiling a new molecular mechanism by which PAK1 regulates whole-body glucose homeostasis. Frontiers Media S.A. 2022-02-11 /pmc/articles/PMC8881144/ /pubmed/35222279 http://dx.doi.org/10.3389/fendo.2022.821849 Text en Copyright © 2022 Merz, Tunduguru, Ahn, Salunkhe, Veluthakal, Hwang, Bhattacharya, McCown, Garcia, Zhou, Oh, Yoder, Elmendorf and Thurmond https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Merz, Karla E.
Tunduguru, Ragadeepthi
Ahn, Miwon
Salunkhe, Vishal A.
Veluthakal, Rajakrishnan
Hwang, Jinhee
Bhattacharya, Supriyo
McCown, Erika M.
Garcia, Pablo A.
Zhou, Chunxue
Oh, Eunjin
Yoder, Stephanie M.
Elmendorf, Jeffrey S.
Thurmond, Debbie C.
Changes in Skeletal Muscle PAK1 Levels Regulate Tissue Crosstalk to Impact Whole Body Glucose Homeostasis
title Changes in Skeletal Muscle PAK1 Levels Regulate Tissue Crosstalk to Impact Whole Body Glucose Homeostasis
title_full Changes in Skeletal Muscle PAK1 Levels Regulate Tissue Crosstalk to Impact Whole Body Glucose Homeostasis
title_fullStr Changes in Skeletal Muscle PAK1 Levels Regulate Tissue Crosstalk to Impact Whole Body Glucose Homeostasis
title_full_unstemmed Changes in Skeletal Muscle PAK1 Levels Regulate Tissue Crosstalk to Impact Whole Body Glucose Homeostasis
title_short Changes in Skeletal Muscle PAK1 Levels Regulate Tissue Crosstalk to Impact Whole Body Glucose Homeostasis
title_sort changes in skeletal muscle pak1 levels regulate tissue crosstalk to impact whole body glucose homeostasis
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8881144/
https://www.ncbi.nlm.nih.gov/pubmed/35222279
http://dx.doi.org/10.3389/fendo.2022.821849
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