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Exosomal hsa_circ_0008925 from Urine Is Related to Chronic Renal Fibrosis

At present, there is no noninvasive biomarker of renal fibrosis. The potential diagnostic value of urinary exosome-derived circRNAs from glomerular disease patients for renal fibrosis is still uncertain. Here, we first detected the expression of hsa_circ_0008925 in TGF-β1-cultured HK-2 cell-derived...

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Autores principales: Cao, Yuhan, Shi, Yuanhui, Wang, Yuwei, Yang, Yanlang, Guo, Wenjun, Zhang, Cuifeng, Pei, Wenjun, Fu, Cong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8881153/
https://www.ncbi.nlm.nih.gov/pubmed/35222740
http://dx.doi.org/10.1155/2022/1899282
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author Cao, Yuhan
Shi, Yuanhui
Wang, Yuwei
Yang, Yanlang
Guo, Wenjun
Zhang, Cuifeng
Pei, Wenjun
Fu, Cong
author_facet Cao, Yuhan
Shi, Yuanhui
Wang, Yuwei
Yang, Yanlang
Guo, Wenjun
Zhang, Cuifeng
Pei, Wenjun
Fu, Cong
author_sort Cao, Yuhan
collection PubMed
description At present, there is no noninvasive biomarker of renal fibrosis. The potential diagnostic value of urinary exosome-derived circRNAs from glomerular disease patients for renal fibrosis is still uncertain. Here, we first detected the expression of hsa_circ_0008925 in TGF-β1-cultured HK-2 cell-derived exosomes. Secondly, we collected urine samples from 95 biopsy-proven glomerular disease patients and 34 healthy controls. The expression of hsa_circ_0008925 was analyzed, and the correlation with renal function and pathological changes was calculated. The receiver operating characteristic (ROC) curve for the diagnosis of renal fibrosis was performed. The results showed that in exosomes derived from TGF-β1-cultured HK-2 cells, the expression of hsa_circ_0008925 was increased compared with normal cultured. Further, the expression level of hsa_circ_0008925 was increased in urinary exosomes from renal fibrosis patients and correlated with serum creatinine, blood urea nitrogen (BUN), estimated glomerular filtration rate, and cystatin C. The level of hsa_circ_0008925 was furthermore correlated with the score of tubulointerstitial fibrosis (TIF) and the score of glomerular sclerosis. The ROC curve showed that hsa_circ_0008925 can diagnose renal fibrosis at a cut-off value of 0.093 with a sensitivity of 52.2% and specificity of 96.4%. In summary, we indicated that urinary exosomal hsa_circ_0008925 could be acted as a noninvasive biomarker for renal fibrosis in glomerular diseases patients.
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spelling pubmed-88811532022-02-26 Exosomal hsa_circ_0008925 from Urine Is Related to Chronic Renal Fibrosis Cao, Yuhan Shi, Yuanhui Wang, Yuwei Yang, Yanlang Guo, Wenjun Zhang, Cuifeng Pei, Wenjun Fu, Cong Dis Markers Research Article At present, there is no noninvasive biomarker of renal fibrosis. The potential diagnostic value of urinary exosome-derived circRNAs from glomerular disease patients for renal fibrosis is still uncertain. Here, we first detected the expression of hsa_circ_0008925 in TGF-β1-cultured HK-2 cell-derived exosomes. Secondly, we collected urine samples from 95 biopsy-proven glomerular disease patients and 34 healthy controls. The expression of hsa_circ_0008925 was analyzed, and the correlation with renal function and pathological changes was calculated. The receiver operating characteristic (ROC) curve for the diagnosis of renal fibrosis was performed. The results showed that in exosomes derived from TGF-β1-cultured HK-2 cells, the expression of hsa_circ_0008925 was increased compared with normal cultured. Further, the expression level of hsa_circ_0008925 was increased in urinary exosomes from renal fibrosis patients and correlated with serum creatinine, blood urea nitrogen (BUN), estimated glomerular filtration rate, and cystatin C. The level of hsa_circ_0008925 was furthermore correlated with the score of tubulointerstitial fibrosis (TIF) and the score of glomerular sclerosis. The ROC curve showed that hsa_circ_0008925 can diagnose renal fibrosis at a cut-off value of 0.093 with a sensitivity of 52.2% and specificity of 96.4%. In summary, we indicated that urinary exosomal hsa_circ_0008925 could be acted as a noninvasive biomarker for renal fibrosis in glomerular diseases patients. Hindawi 2022-02-18 /pmc/articles/PMC8881153/ /pubmed/35222740 http://dx.doi.org/10.1155/2022/1899282 Text en Copyright © 2022 Yuhan Cao et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Cao, Yuhan
Shi, Yuanhui
Wang, Yuwei
Yang, Yanlang
Guo, Wenjun
Zhang, Cuifeng
Pei, Wenjun
Fu, Cong
Exosomal hsa_circ_0008925 from Urine Is Related to Chronic Renal Fibrosis
title Exosomal hsa_circ_0008925 from Urine Is Related to Chronic Renal Fibrosis
title_full Exosomal hsa_circ_0008925 from Urine Is Related to Chronic Renal Fibrosis
title_fullStr Exosomal hsa_circ_0008925 from Urine Is Related to Chronic Renal Fibrosis
title_full_unstemmed Exosomal hsa_circ_0008925 from Urine Is Related to Chronic Renal Fibrosis
title_short Exosomal hsa_circ_0008925 from Urine Is Related to Chronic Renal Fibrosis
title_sort exosomal hsa_circ_0008925 from urine is related to chronic renal fibrosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8881153/
https://www.ncbi.nlm.nih.gov/pubmed/35222740
http://dx.doi.org/10.1155/2022/1899282
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