Selection of Aspergillus fumigatus isolates carrying the G448S substitution in CYP51A gene after long-term treatment with voriconazole in an immunocompromised patient

We present a case of a 55-year-old man with a heart transplant who acquired Invasive Aspergillosis by Aspergillus fumigatus with the focus in the kidney. During about two years of antifungal treatment, most of the time with voriconazole, it was possible to obtain nine isolates of A. fumigatus, with...

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Autores principales: Pontes, Laís, Gualtieri Beraquet, Caio Augusto, Arai, Teppei, Watanabe, Akira, Moretti, Maria Luiza, Schreiber, Angelica Zaninelli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Case Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8881195/
https://www.ncbi.nlm.nih.gov/pubmed/35242508
http://dx.doi.org/10.1016/j.mmcr.2022.02.002
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author Pontes, Laís
Gualtieri Beraquet, Caio Augusto
Arai, Teppei
Watanabe, Akira
Moretti, Maria Luiza
Schreiber, Angelica Zaninelli
author_facet Pontes, Laís
Gualtieri Beraquet, Caio Augusto
Arai, Teppei
Watanabe, Akira
Moretti, Maria Luiza
Schreiber, Angelica Zaninelli
author_sort Pontes, Laís
collection PubMed
description We present a case of a 55-year-old man with a heart transplant who acquired Invasive Aspergillosis by Aspergillus fumigatus with the focus in the kidney. During about two years of antifungal treatment, most of the time with voriconazole, it was possible to obtain nine isolates of A. fumigatus, with the same genotypic characteristics, but with an increase in MIC for several azoles. The two last isolates presented high MICs for Voriconazole (>8 μg/mL>). Sequencing of the CYP51A gene showed G448S amino acid substitution in the same two isolates. In long-term treatments with antifungals, it would be important to regularly evaluate the susceptibility of isolated strains, as resistance to azoles has been increasingly described around the world.
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spelling pubmed-88811952022-03-02 Selection of Aspergillus fumigatus isolates carrying the G448S substitution in CYP51A gene after long-term treatment with voriconazole in an immunocompromised patient Pontes, Laís Gualtieri Beraquet, Caio Augusto Arai, Teppei Watanabe, Akira Moretti, Maria Luiza Schreiber, Angelica Zaninelli Med Mycol Case Rep Case Report We present a case of a 55-year-old man with a heart transplant who acquired Invasive Aspergillosis by Aspergillus fumigatus with the focus in the kidney. During about two years of antifungal treatment, most of the time with voriconazole, it was possible to obtain nine isolates of A. fumigatus, with the same genotypic characteristics, but with an increase in MIC for several azoles. The two last isolates presented high MICs for Voriconazole (>8 μg/mL>). Sequencing of the CYP51A gene showed G448S amino acid substitution in the same two isolates. In long-term treatments with antifungals, it would be important to regularly evaluate the susceptibility of isolated strains, as resistance to azoles has been increasingly described around the world. Elsevier 2022-02-18 /pmc/articles/PMC8881195/ /pubmed/35242508 http://dx.doi.org/10.1016/j.mmcr.2022.02.002 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Case Report
Pontes, Laís
Gualtieri Beraquet, Caio Augusto
Arai, Teppei
Watanabe, Akira
Moretti, Maria Luiza
Schreiber, Angelica Zaninelli
Selection of Aspergillus fumigatus isolates carrying the G448S substitution in CYP51A gene after long-term treatment with voriconazole in an immunocompromised patient
title Selection of Aspergillus fumigatus isolates carrying the G448S substitution in CYP51A gene after long-term treatment with voriconazole in an immunocompromised patient
title_full Selection of Aspergillus fumigatus isolates carrying the G448S substitution in CYP51A gene after long-term treatment with voriconazole in an immunocompromised patient
title_fullStr Selection of Aspergillus fumigatus isolates carrying the G448S substitution in CYP51A gene after long-term treatment with voriconazole in an immunocompromised patient
title_full_unstemmed Selection of Aspergillus fumigatus isolates carrying the G448S substitution in CYP51A gene after long-term treatment with voriconazole in an immunocompromised patient
title_short Selection of Aspergillus fumigatus isolates carrying the G448S substitution in CYP51A gene after long-term treatment with voriconazole in an immunocompromised patient
title_sort selection of aspergillus fumigatus isolates carrying the g448s substitution in cyp51a gene after long-term treatment with voriconazole in an immunocompromised patient
topic Case Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8881195/
https://www.ncbi.nlm.nih.gov/pubmed/35242508
http://dx.doi.org/10.1016/j.mmcr.2022.02.002
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