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Platelet-derived growth factor-BB regenerates functional periodontal ligament in the tooth replantation

Tooth ankylosis is a pathological condition of periodontal ligament (PDL) restoration after tooth replantation. Platelet-derived growth factor-BB (PDGF-BB) has been proposed as a promising factor for preventing tooth ankylosis. Using rat tooth replantation model, we investigated whether PDGF-BB acce...

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Autores principales: Komatsu, Koichiro, Ideno, Hisashi, Shibata, Tatsuya, Nakashima, Kazuhisa, Nifuji, Akira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8881622/
https://www.ncbi.nlm.nih.gov/pubmed/35217688
http://dx.doi.org/10.1038/s41598-022-06865-6
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author Komatsu, Koichiro
Ideno, Hisashi
Shibata, Tatsuya
Nakashima, Kazuhisa
Nifuji, Akira
author_facet Komatsu, Koichiro
Ideno, Hisashi
Shibata, Tatsuya
Nakashima, Kazuhisa
Nifuji, Akira
author_sort Komatsu, Koichiro
collection PubMed
description Tooth ankylosis is a pathological condition of periodontal ligament (PDL) restoration after tooth replantation. Platelet-derived growth factor-BB (PDGF-BB) has been proposed as a promising factor for preventing tooth ankylosis. Using rat tooth replantation model, we investigated whether PDGF-BB accelerates the repair of PDL after tooth replantation without ankylosis, and its molecular mechanisms. In PDGF-BB pretreated replanted teeth (PDGF-BB group), ankylosis was markedly reduced and functionally organized PDL collagen fibers were restored; the mechanical strength of the healing PDL was restored to an average of 76% of that in non-replanted normal teeth at 21 days. The numbers of PDGF-Rβ- and BrdU-positive cells in the periodontal tissues of the PDGF-BB group were greater than those of atelocollagen pretreated replanted teeth (AC group). Moreover, in the PDGF-BB group, the periodontal tissues had fewer osteocalcin-positive cells and decreased number of nuclear β-catenin-positive cells compared to those in the AC group. In vitro analyses showed that PDGF-BB increased the proliferation and migration of human periodontal fibroblasts. PDGF-BB downregulated mRNA expressions of RUNX2 and ALP, and inhibited upregulatory effects of Wnt3a on β-catenin, AXIN2, RUNX2, COL1A1, and ALP mRNA expressions. These findings indicate that in tooth replantation, topical PDGF-BB treatment enhances cell proliferation and migration, and inhibits canonical Wnt signaling activation in bone-tooth ankylosis, leading to occlusal loading of the PDL tissues and subsequent functional restoration of the healing PDL. This suggests a possible clinical application of PDGF-BB to reduce ankylosis after tooth replantation and promote proper regeneration of PDL.
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spelling pubmed-88816222022-03-01 Platelet-derived growth factor-BB regenerates functional periodontal ligament in the tooth replantation Komatsu, Koichiro Ideno, Hisashi Shibata, Tatsuya Nakashima, Kazuhisa Nifuji, Akira Sci Rep Article Tooth ankylosis is a pathological condition of periodontal ligament (PDL) restoration after tooth replantation. Platelet-derived growth factor-BB (PDGF-BB) has been proposed as a promising factor for preventing tooth ankylosis. Using rat tooth replantation model, we investigated whether PDGF-BB accelerates the repair of PDL after tooth replantation without ankylosis, and its molecular mechanisms. In PDGF-BB pretreated replanted teeth (PDGF-BB group), ankylosis was markedly reduced and functionally organized PDL collagen fibers were restored; the mechanical strength of the healing PDL was restored to an average of 76% of that in non-replanted normal teeth at 21 days. The numbers of PDGF-Rβ- and BrdU-positive cells in the periodontal tissues of the PDGF-BB group were greater than those of atelocollagen pretreated replanted teeth (AC group). Moreover, in the PDGF-BB group, the periodontal tissues had fewer osteocalcin-positive cells and decreased number of nuclear β-catenin-positive cells compared to those in the AC group. In vitro analyses showed that PDGF-BB increased the proliferation and migration of human periodontal fibroblasts. PDGF-BB downregulated mRNA expressions of RUNX2 and ALP, and inhibited upregulatory effects of Wnt3a on β-catenin, AXIN2, RUNX2, COL1A1, and ALP mRNA expressions. These findings indicate that in tooth replantation, topical PDGF-BB treatment enhances cell proliferation and migration, and inhibits canonical Wnt signaling activation in bone-tooth ankylosis, leading to occlusal loading of the PDL tissues and subsequent functional restoration of the healing PDL. This suggests a possible clinical application of PDGF-BB to reduce ankylosis after tooth replantation and promote proper regeneration of PDL. Nature Publishing Group UK 2022-02-25 /pmc/articles/PMC8881622/ /pubmed/35217688 http://dx.doi.org/10.1038/s41598-022-06865-6 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Komatsu, Koichiro
Ideno, Hisashi
Shibata, Tatsuya
Nakashima, Kazuhisa
Nifuji, Akira
Platelet-derived growth factor-BB regenerates functional periodontal ligament in the tooth replantation
title Platelet-derived growth factor-BB regenerates functional periodontal ligament in the tooth replantation
title_full Platelet-derived growth factor-BB regenerates functional periodontal ligament in the tooth replantation
title_fullStr Platelet-derived growth factor-BB regenerates functional periodontal ligament in the tooth replantation
title_full_unstemmed Platelet-derived growth factor-BB regenerates functional periodontal ligament in the tooth replantation
title_short Platelet-derived growth factor-BB regenerates functional periodontal ligament in the tooth replantation
title_sort platelet-derived growth factor-bb regenerates functional periodontal ligament in the tooth replantation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8881622/
https://www.ncbi.nlm.nih.gov/pubmed/35217688
http://dx.doi.org/10.1038/s41598-022-06865-6
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