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Overexpression of cytosolic long noncoding RNA cytb protects against pressure-overload-induced heart failure via sponging microRNA-103-3p
Long noncoding RNAs (lncRNAs) play crucial roles in cardiovascular diseases. To date, only limited studies have reported the role of mitochondria-derived lncRNAs in heart failure (HF). In the current study, recombinant adeno-associated virus 9 was used to manipulate lncRNA cytb (lnccytb) expression...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society of Gene & Cell Therapy
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8881631/ https://www.ncbi.nlm.nih.gov/pubmed/35251768 http://dx.doi.org/10.1016/j.omtn.2022.02.002 |
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author | Zhang, Xudong Yuan, Shuai Liu, Jingbo Tang, Yuyan Wang, Yan Zhan, Jiabing Fan, Jiahui Nie, Xiang Zhao, Yanru Wen, Zheng Li, Huaping Chen, Chen Wang, Dao Wen |
author_facet | Zhang, Xudong Yuan, Shuai Liu, Jingbo Tang, Yuyan Wang, Yan Zhan, Jiabing Fan, Jiahui Nie, Xiang Zhao, Yanru Wen, Zheng Li, Huaping Chen, Chen Wang, Dao Wen |
author_sort | Zhang, Xudong |
collection | PubMed |
description | Long noncoding RNAs (lncRNAs) play crucial roles in cardiovascular diseases. To date, only limited studies have reported the role of mitochondria-derived lncRNAs in heart failure (HF). In the current study, recombinant adeno-associated virus 9 was used to manipulate lncRNA cytb (lnccytb) expression in vivo. Fluorescence in situ hybridization (FISH) assay was used to determine the location of lnccytb, while microRNA (miRNA) sequencing and bioinformatics analyses were applied to identify the downstream targets. The competitive endogenous RNA (ceRNA) function of lnccytb was evaluated by biotin-coupled miRNA pull-down assays and luciferase reporter assays. Results showed that lnccytb expression was decreased in the heart of mice with transverse aortic constriction (TAC), as well as in the heart and plasma of patients with HF. FISH assay and absolute RNA quantification via real-time reverse transcription PCR suggested that the reduction of the lnccytb transcripts mainly occurred in the cytosol. Upregulation of cytosolic lnccytb attenuated cardiac dysfunction in TAC mice. Moreover, overexpression of cytosolic lnccytb in cardiomyocytes alleviated isoprenaline-induced reactive oxidative species (ROS) production and hypertrophy. Mechanistically, lnccytb acted as a ceRNA via sponging miR-103-3p, ultimately mitigating the suppression of PTEN by miR-103-3p. In summary, we demonstrated that the overexpression of cytosolic lnccytb could ameliorate HF. |
format | Online Article Text |
id | pubmed-8881631 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Society of Gene & Cell Therapy |
record_format | MEDLINE/PubMed |
spelling | pubmed-88816312022-03-04 Overexpression of cytosolic long noncoding RNA cytb protects against pressure-overload-induced heart failure via sponging microRNA-103-3p Zhang, Xudong Yuan, Shuai Liu, Jingbo Tang, Yuyan Wang, Yan Zhan, Jiabing Fan, Jiahui Nie, Xiang Zhao, Yanru Wen, Zheng Li, Huaping Chen, Chen Wang, Dao Wen Mol Ther Nucleic Acids Original Article Long noncoding RNAs (lncRNAs) play crucial roles in cardiovascular diseases. To date, only limited studies have reported the role of mitochondria-derived lncRNAs in heart failure (HF). In the current study, recombinant adeno-associated virus 9 was used to manipulate lncRNA cytb (lnccytb) expression in vivo. Fluorescence in situ hybridization (FISH) assay was used to determine the location of lnccytb, while microRNA (miRNA) sequencing and bioinformatics analyses were applied to identify the downstream targets. The competitive endogenous RNA (ceRNA) function of lnccytb was evaluated by biotin-coupled miRNA pull-down assays and luciferase reporter assays. Results showed that lnccytb expression was decreased in the heart of mice with transverse aortic constriction (TAC), as well as in the heart and plasma of patients with HF. FISH assay and absolute RNA quantification via real-time reverse transcription PCR suggested that the reduction of the lnccytb transcripts mainly occurred in the cytosol. Upregulation of cytosolic lnccytb attenuated cardiac dysfunction in TAC mice. Moreover, overexpression of cytosolic lnccytb in cardiomyocytes alleviated isoprenaline-induced reactive oxidative species (ROS) production and hypertrophy. Mechanistically, lnccytb acted as a ceRNA via sponging miR-103-3p, ultimately mitigating the suppression of PTEN by miR-103-3p. In summary, we demonstrated that the overexpression of cytosolic lnccytb could ameliorate HF. American Society of Gene & Cell Therapy 2022-02-10 /pmc/articles/PMC8881631/ /pubmed/35251768 http://dx.doi.org/10.1016/j.omtn.2022.02.002 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Zhang, Xudong Yuan, Shuai Liu, Jingbo Tang, Yuyan Wang, Yan Zhan, Jiabing Fan, Jiahui Nie, Xiang Zhao, Yanru Wen, Zheng Li, Huaping Chen, Chen Wang, Dao Wen Overexpression of cytosolic long noncoding RNA cytb protects against pressure-overload-induced heart failure via sponging microRNA-103-3p |
title | Overexpression of cytosolic long noncoding RNA cytb protects against pressure-overload-induced heart failure via sponging microRNA-103-3p |
title_full | Overexpression of cytosolic long noncoding RNA cytb protects against pressure-overload-induced heart failure via sponging microRNA-103-3p |
title_fullStr | Overexpression of cytosolic long noncoding RNA cytb protects against pressure-overload-induced heart failure via sponging microRNA-103-3p |
title_full_unstemmed | Overexpression of cytosolic long noncoding RNA cytb protects against pressure-overload-induced heart failure via sponging microRNA-103-3p |
title_short | Overexpression of cytosolic long noncoding RNA cytb protects against pressure-overload-induced heart failure via sponging microRNA-103-3p |
title_sort | overexpression of cytosolic long noncoding rna cytb protects against pressure-overload-induced heart failure via sponging microrna-103-3p |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8881631/ https://www.ncbi.nlm.nih.gov/pubmed/35251768 http://dx.doi.org/10.1016/j.omtn.2022.02.002 |
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