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Interferograms plotted with reference change value (RCV) may facilitate the management of hemolyzed samples

BACKGROUND: The European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) Working Group for Preanalytical Phase (WG-PRE) have recommended an algorithm based on the reference change value (RCV) to evaluate hemolysis. We utilized this algorithm to analyze hemolysis-sensitive parameters....

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Autores principales: Uçar, Kamil Taha, Çat, Abdulkadir, Gümüş, Alper, Nurlu, Nilhan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Society of Medical Biochemists of Serbia, Belgrade 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8882018/
https://www.ncbi.nlm.nih.gov/pubmed/35291494
http://dx.doi.org/10.5937/jomb0-31250
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author Uçar, Kamil Taha
Çat, Abdulkadir
Gümüş, Alper
Nurlu, Nilhan
author_facet Uçar, Kamil Taha
Çat, Abdulkadir
Gümüş, Alper
Nurlu, Nilhan
author_sort Uçar, Kamil Taha
collection PubMed
description BACKGROUND: The European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) Working Group for Preanalytical Phase (WG-PRE) have recommended an algorithm based on the reference change value (RCV) to evaluate hemolysis. We utilized this algorithm to analyze hemolysis-sensitive parameters. METHODS: Two tubes of blood were collected from each of the 10 participants, one of which was subjected to mechanical trauma while the other was centrifuged directly. Subsequently, the samples were diluted with the participant's hemolyzed sample to obtain the desired hemoglobin concentrations (0, 1, 2, 4, 6, 8, and 10 g/L). ALT, AST, K, LDH, T. Bil tests were performed using Beckman Coulter AU680 analyzer. The analytical and clinical cut-offs were based on the biological variation for the allowable imprecision and RCV. The algorithms could report the values directly below the analytical cut-off or those between the analytical and clinical cut-offs with comments. If the change was above the clinical cut-off, the test was rejected. The linear regression was used for interferograms, and the hemoglobin concentrations corresponding to cut-offs were calculated via the interferograms. RESULTS: The RCV was calculated as 29.6% for ALT. Therefore, ALT should be rejected in samples containing >5.9 g/L hemoglobin. The RCVs for AST, K, LDH, and T. Bil were calculated as 27.9%, 12.1%, 19.2%, and 61.2%, while the samples' hemoglobin concentrations for test rejection were 0.8, 1.6, 0.5, and 2.2 g/L, respectively. CONCLUSIONS: Algorithms prepared with RCV could provide evidence-based results and objectively manage hemolyzed samples.
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spelling pubmed-88820182022-03-14 Interferograms plotted with reference change value (RCV) may facilitate the management of hemolyzed samples Uçar, Kamil Taha Çat, Abdulkadir Gümüş, Alper Nurlu, Nilhan J Med Biochem Original Paper BACKGROUND: The European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) Working Group for Preanalytical Phase (WG-PRE) have recommended an algorithm based on the reference change value (RCV) to evaluate hemolysis. We utilized this algorithm to analyze hemolysis-sensitive parameters. METHODS: Two tubes of blood were collected from each of the 10 participants, one of which was subjected to mechanical trauma while the other was centrifuged directly. Subsequently, the samples were diluted with the participant's hemolyzed sample to obtain the desired hemoglobin concentrations (0, 1, 2, 4, 6, 8, and 10 g/L). ALT, AST, K, LDH, T. Bil tests were performed using Beckman Coulter AU680 analyzer. The analytical and clinical cut-offs were based on the biological variation for the allowable imprecision and RCV. The algorithms could report the values directly below the analytical cut-off or those between the analytical and clinical cut-offs with comments. If the change was above the clinical cut-off, the test was rejected. The linear regression was used for interferograms, and the hemoglobin concentrations corresponding to cut-offs were calculated via the interferograms. RESULTS: The RCV was calculated as 29.6% for ALT. Therefore, ALT should be rejected in samples containing >5.9 g/L hemoglobin. The RCVs for AST, K, LDH, and T. Bil were calculated as 27.9%, 12.1%, 19.2%, and 61.2%, while the samples' hemoglobin concentrations for test rejection were 0.8, 1.6, 0.5, and 2.2 g/L, respectively. CONCLUSIONS: Algorithms prepared with RCV could provide evidence-based results and objectively manage hemolyzed samples. Society of Medical Biochemists of Serbia, Belgrade 2022-02-02 2022-02-02 /pmc/articles/PMC8882018/ /pubmed/35291494 http://dx.doi.org/10.5937/jomb0-31250 Text en 2022 Kamil Taha Uçar, Abdulkadir Çat, Alper Gümüş, Nilhan Nurlu, published by CEON/CEES https://creativecommons.org/licenses/by/4.0/This work is licensed under the Creative Commons Attribution 4.0 License.
spellingShingle Original Paper
Uçar, Kamil Taha
Çat, Abdulkadir
Gümüş, Alper
Nurlu, Nilhan
Interferograms plotted with reference change value (RCV) may facilitate the management of hemolyzed samples
title Interferograms plotted with reference change value (RCV) may facilitate the management of hemolyzed samples
title_full Interferograms plotted with reference change value (RCV) may facilitate the management of hemolyzed samples
title_fullStr Interferograms plotted with reference change value (RCV) may facilitate the management of hemolyzed samples
title_full_unstemmed Interferograms plotted with reference change value (RCV) may facilitate the management of hemolyzed samples
title_short Interferograms plotted with reference change value (RCV) may facilitate the management of hemolyzed samples
title_sort interferograms plotted with reference change value (rcv) may facilitate the management of hemolyzed samples
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8882018/
https://www.ncbi.nlm.nih.gov/pubmed/35291494
http://dx.doi.org/10.5937/jomb0-31250
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