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Virus MIP-composites for SARS-CoV-2 detection in the aquatic environment

SARS-CoV-2 is the virus responsible for causing the global COVID-19 pandemic. Identifying the presence of this virus in the environment could potentially improve the effectiveness of disease control measures. Environmental SARS-CoV-2 monitoring may become increasingly demanded in areas where the ava...

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Autores principales: Sukjee, Wannisa, Thitithanyanont, Arunee, Manopwisedjaroen, Suwimon, Seetaha, Supaphorn, Thepparit, Chutima, Sangma, Chak
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8882038/
https://www.ncbi.nlm.nih.gov/pubmed/35250128
http://dx.doi.org/10.1016/j.matlet.2022.131973
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author Sukjee, Wannisa
Thitithanyanont, Arunee
Manopwisedjaroen, Suwimon
Seetaha, Supaphorn
Thepparit, Chutima
Sangma, Chak
author_facet Sukjee, Wannisa
Thitithanyanont, Arunee
Manopwisedjaroen, Suwimon
Seetaha, Supaphorn
Thepparit, Chutima
Sangma, Chak
author_sort Sukjee, Wannisa
collection PubMed
description SARS-CoV-2 is the virus responsible for causing the global COVID-19 pandemic. Identifying the presence of this virus in the environment could potentially improve the effectiveness of disease control measures. Environmental SARS-CoV-2 monitoring may become increasingly demanded in areas where the available testing methods are ineffective. In this study, we present an electrochemical polymer composites biosensor for measuring SARS-CoV-2 whole-virus particles in the environment. The sensitized layer was prepared from molecularly imprinted polymer (MIP) composites of inactivated SARS-CoV-2. Testing demonstrated increased sensor signaling with SARS-CoV-2 specifically, while lower responses were observed to the negative controls, H5N1 influenza A virus and non-imprinted polymers (NIPs). This sensor detected SARS-CoV-2 at concentrations as low as 0.1 fM in buffer and samples prepared from reservoir water with a 3 log-scale linearity.
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spelling pubmed-88820382022-02-28 Virus MIP-composites for SARS-CoV-2 detection in the aquatic environment Sukjee, Wannisa Thitithanyanont, Arunee Manopwisedjaroen, Suwimon Seetaha, Supaphorn Thepparit, Chutima Sangma, Chak Mater Lett Article SARS-CoV-2 is the virus responsible for causing the global COVID-19 pandemic. Identifying the presence of this virus in the environment could potentially improve the effectiveness of disease control measures. Environmental SARS-CoV-2 monitoring may become increasingly demanded in areas where the available testing methods are ineffective. In this study, we present an electrochemical polymer composites biosensor for measuring SARS-CoV-2 whole-virus particles in the environment. The sensitized layer was prepared from molecularly imprinted polymer (MIP) composites of inactivated SARS-CoV-2. Testing demonstrated increased sensor signaling with SARS-CoV-2 specifically, while lower responses were observed to the negative controls, H5N1 influenza A virus and non-imprinted polymers (NIPs). This sensor detected SARS-CoV-2 at concentrations as low as 0.1 fM in buffer and samples prepared from reservoir water with a 3 log-scale linearity. Elsevier B.V. 2022-05-15 2022-02-26 /pmc/articles/PMC8882038/ /pubmed/35250128 http://dx.doi.org/10.1016/j.matlet.2022.131973 Text en © 2022 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Sukjee, Wannisa
Thitithanyanont, Arunee
Manopwisedjaroen, Suwimon
Seetaha, Supaphorn
Thepparit, Chutima
Sangma, Chak
Virus MIP-composites for SARS-CoV-2 detection in the aquatic environment
title Virus MIP-composites for SARS-CoV-2 detection in the aquatic environment
title_full Virus MIP-composites for SARS-CoV-2 detection in the aquatic environment
title_fullStr Virus MIP-composites for SARS-CoV-2 detection in the aquatic environment
title_full_unstemmed Virus MIP-composites for SARS-CoV-2 detection in the aquatic environment
title_short Virus MIP-composites for SARS-CoV-2 detection in the aquatic environment
title_sort virus mip-composites for sars-cov-2 detection in the aquatic environment
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8882038/
https://www.ncbi.nlm.nih.gov/pubmed/35250128
http://dx.doi.org/10.1016/j.matlet.2022.131973
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