CircCYP24A1 hampered malignant phenotype of renal cancer carcinoma through modulating CMTM-4 expression via sponging miR-421

Renal cell carcinoma (RCC) is a lethal urinary malignancy. Circular RNAs (circRNAs) contribute to the malignant phenotype and progression of several types of human cancers, including RCC. In this study, we identified relatively low hsa_circ_0060927 (circCYP24A1) expression in RCC tissue through high...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Xiaorong, Zhou, Jiale, Zhao, Ling, Yang, Zhaolin, Yang, Chen, Chen, Yonghui, Xue, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8882186/
https://www.ncbi.nlm.nih.gov/pubmed/35220395
http://dx.doi.org/10.1038/s41419-022-04623-0
_version_ 1784659640828559360
author Wu, Xiaorong
Zhou, Jiale
Zhao, Ling
Yang, Zhaolin
Yang, Chen
Chen, Yonghui
Xue, Wei
author_facet Wu, Xiaorong
Zhou, Jiale
Zhao, Ling
Yang, Zhaolin
Yang, Chen
Chen, Yonghui
Xue, Wei
author_sort Wu, Xiaorong
collection PubMed
description Renal cell carcinoma (RCC) is a lethal urinary malignancy. Circular RNAs (circRNAs) contribute to the malignant phenotype and progression of several types of human cancers, including RCC. In this study, we identified relatively low hsa_circ_0060927 (circCYP24A1) expression in RCC tissue through high-throughput sequencing and RT–qPCR. Fluorescence in situ hybridization (FISH) was used to validate the expression and subcellular localization of circCYP24A1 in RCC tissues. CCK-8, Transwell, EdU, and wound-healing assays indicated that circCYP24A1 overexpression inhibited the proliferation, invasion, and migration of RCC cells. Dual-luciferase reporter, RNA immunoprecipitation (RIP), FISH, and RNA-pulldown assays verified that circCYP24A1 inhibited RCC progression by sponging miR-421, thus inducing CMTM-4 expression. Xenograft assays and metastasis models further indicated that circCYP24A1 significantly inhibited the metastasis and proliferation of RCC cells in vivo. Taken together, circCYP24A1 is a prognosis-related circRNA in RCC that functions through the circCYP24A1/miR-421/CMTM-4 axis to modulate RCC progression.
format Online
Article
Text
id pubmed-8882186
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-88821862022-03-17 CircCYP24A1 hampered malignant phenotype of renal cancer carcinoma through modulating CMTM-4 expression via sponging miR-421 Wu, Xiaorong Zhou, Jiale Zhao, Ling Yang, Zhaolin Yang, Chen Chen, Yonghui Xue, Wei Cell Death Dis Article Renal cell carcinoma (RCC) is a lethal urinary malignancy. Circular RNAs (circRNAs) contribute to the malignant phenotype and progression of several types of human cancers, including RCC. In this study, we identified relatively low hsa_circ_0060927 (circCYP24A1) expression in RCC tissue through high-throughput sequencing and RT–qPCR. Fluorescence in situ hybridization (FISH) was used to validate the expression and subcellular localization of circCYP24A1 in RCC tissues. CCK-8, Transwell, EdU, and wound-healing assays indicated that circCYP24A1 overexpression inhibited the proliferation, invasion, and migration of RCC cells. Dual-luciferase reporter, RNA immunoprecipitation (RIP), FISH, and RNA-pulldown assays verified that circCYP24A1 inhibited RCC progression by sponging miR-421, thus inducing CMTM-4 expression. Xenograft assays and metastasis models further indicated that circCYP24A1 significantly inhibited the metastasis and proliferation of RCC cells in vivo. Taken together, circCYP24A1 is a prognosis-related circRNA in RCC that functions through the circCYP24A1/miR-421/CMTM-4 axis to modulate RCC progression. Nature Publishing Group UK 2022-02-26 /pmc/articles/PMC8882186/ /pubmed/35220395 http://dx.doi.org/10.1038/s41419-022-04623-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Wu, Xiaorong
Zhou, Jiale
Zhao, Ling
Yang, Zhaolin
Yang, Chen
Chen, Yonghui
Xue, Wei
CircCYP24A1 hampered malignant phenotype of renal cancer carcinoma through modulating CMTM-4 expression via sponging miR-421
title CircCYP24A1 hampered malignant phenotype of renal cancer carcinoma through modulating CMTM-4 expression via sponging miR-421
title_full CircCYP24A1 hampered malignant phenotype of renal cancer carcinoma through modulating CMTM-4 expression via sponging miR-421
title_fullStr CircCYP24A1 hampered malignant phenotype of renal cancer carcinoma through modulating CMTM-4 expression via sponging miR-421
title_full_unstemmed CircCYP24A1 hampered malignant phenotype of renal cancer carcinoma through modulating CMTM-4 expression via sponging miR-421
title_short CircCYP24A1 hampered malignant phenotype of renal cancer carcinoma through modulating CMTM-4 expression via sponging miR-421
title_sort circcyp24a1 hampered malignant phenotype of renal cancer carcinoma through modulating cmtm-4 expression via sponging mir-421
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8882186/
https://www.ncbi.nlm.nih.gov/pubmed/35220395
http://dx.doi.org/10.1038/s41419-022-04623-0
work_keys_str_mv AT wuxiaorong circcyp24a1hamperedmalignantphenotypeofrenalcancercarcinomathroughmodulatingcmtm4expressionviaspongingmir421
AT zhoujiale circcyp24a1hamperedmalignantphenotypeofrenalcancercarcinomathroughmodulatingcmtm4expressionviaspongingmir421
AT zhaoling circcyp24a1hamperedmalignantphenotypeofrenalcancercarcinomathroughmodulatingcmtm4expressionviaspongingmir421
AT yangzhaolin circcyp24a1hamperedmalignantphenotypeofrenalcancercarcinomathroughmodulatingcmtm4expressionviaspongingmir421
AT yangchen circcyp24a1hamperedmalignantphenotypeofrenalcancercarcinomathroughmodulatingcmtm4expressionviaspongingmir421
AT chenyonghui circcyp24a1hamperedmalignantphenotypeofrenalcancercarcinomathroughmodulatingcmtm4expressionviaspongingmir421
AT xuewei circcyp24a1hamperedmalignantphenotypeofrenalcancercarcinomathroughmodulatingcmtm4expressionviaspongingmir421

Ejemplares similares