Cargando…

Norepinephrine promotes glioma cell migration through up-regulating the expression of Twist1

BACKGROUND: Glioma cells are characterized by high migration ability, resulting in aggressive growth of the tumors and poor prognosis of patients. It has been reported that the stress-induced hormone norepinephrine (NE) contributes to tumor progression through mediating a number of important biologi...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Xue, Wang, Ying, Xie, Fang, Song, Zi-Tian, Zhang, Zi-Qian, Zhao, Yun, Wang, Shi-Da, Hu, Hui, Zhang, Yan-Shu, Qian, Ling-Jia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8882280/
https://www.ncbi.nlm.nih.gov/pubmed/35219305
http://dx.doi.org/10.1186/s12885-022-09330-9
_version_ 1784659661090193408
author Wang, Xue
Wang, Ying
Xie, Fang
Song, Zi-Tian
Zhang, Zi-Qian
Zhao, Yun
Wang, Shi-Da
Hu, Hui
Zhang, Yan-Shu
Qian, Ling-Jia
author_facet Wang, Xue
Wang, Ying
Xie, Fang
Song, Zi-Tian
Zhang, Zi-Qian
Zhao, Yun
Wang, Shi-Da
Hu, Hui
Zhang, Yan-Shu
Qian, Ling-Jia
author_sort Wang, Xue
collection PubMed
description BACKGROUND: Glioma cells are characterized by high migration ability, resulting in aggressive growth of the tumors and poor prognosis of patients. It has been reported that the stress-induced hormone norepinephrine (NE) contributes to tumor progression through mediating a number of important biological processes in various cancers. However, the role of NE in the regulation of glioma migration is still unclear. Epithelial-to-mesenchymal transition (EMT) is one of the most important steps for tumor migration and metastasis. Twist1, as a key regulator of EMT, has been found to be elevated during glioma migration. But it is still unknown whether Twist1 is involved in the effect of NE on the migration of glioma cells. METHODS: Wound healing assay and transwell assay were conducted to evaluate the migration of glioma cells upon different treatments. The mesenchymal-like phenotype and the expression of Twist1 after NE treatment were assessed by cell diameters, real-time PCR, western blot and immunofluorescence staining. The gain-and loss-of-function experiments were carried out to investigate the biological function of Twist1 in the migration induced by NE. Finally, the clinical significance of Twist1 was explored among three public glioma datasets. RESULTS: In this study, our finding revealed a facilitative effect of NE on glioma cell migration in a β-adrenergic receptor (ADRB)-dependent way. Mechanistically, NE induced mesenchymal-like phenotype and the expression of Twist1. Twist1 overexpression promoted glioma cells migration, while knockdown of Twist1 abolished the discrepancy in the migration ability between NE treated glioma cells and control cells. In addition, the clinical analysis demonstrated that Twist1 was up-regulated in malignant gliomas and recurrent gliomas, and predicted a poor prognosis of glioma patients. CONCLUSIONS: NE enhanced the migration ability of glioma cells through elevating the expression of Twist1. Our finding may provide potential therapeutic target for protecting patients with glioma from the detrimental effects of stress biology on the tumor progression. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12885-022-09330-9.
format Online
Article
Text
id pubmed-8882280
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-88822802022-02-28 Norepinephrine promotes glioma cell migration through up-regulating the expression of Twist1 Wang, Xue Wang, Ying Xie, Fang Song, Zi-Tian Zhang, Zi-Qian Zhao, Yun Wang, Shi-Da Hu, Hui Zhang, Yan-Shu Qian, Ling-Jia BMC Cancer Research BACKGROUND: Glioma cells are characterized by high migration ability, resulting in aggressive growth of the tumors and poor prognosis of patients. It has been reported that the stress-induced hormone norepinephrine (NE) contributes to tumor progression through mediating a number of important biological processes in various cancers. However, the role of NE in the regulation of glioma migration is still unclear. Epithelial-to-mesenchymal transition (EMT) is one of the most important steps for tumor migration and metastasis. Twist1, as a key regulator of EMT, has been found to be elevated during glioma migration. But it is still unknown whether Twist1 is involved in the effect of NE on the migration of glioma cells. METHODS: Wound healing assay and transwell assay were conducted to evaluate the migration of glioma cells upon different treatments. The mesenchymal-like phenotype and the expression of Twist1 after NE treatment were assessed by cell diameters, real-time PCR, western blot and immunofluorescence staining. The gain-and loss-of-function experiments were carried out to investigate the biological function of Twist1 in the migration induced by NE. Finally, the clinical significance of Twist1 was explored among three public glioma datasets. RESULTS: In this study, our finding revealed a facilitative effect of NE on glioma cell migration in a β-adrenergic receptor (ADRB)-dependent way. Mechanistically, NE induced mesenchymal-like phenotype and the expression of Twist1. Twist1 overexpression promoted glioma cells migration, while knockdown of Twist1 abolished the discrepancy in the migration ability between NE treated glioma cells and control cells. In addition, the clinical analysis demonstrated that Twist1 was up-regulated in malignant gliomas and recurrent gliomas, and predicted a poor prognosis of glioma patients. CONCLUSIONS: NE enhanced the migration ability of glioma cells through elevating the expression of Twist1. Our finding may provide potential therapeutic target for protecting patients with glioma from the detrimental effects of stress biology on the tumor progression. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12885-022-09330-9. BioMed Central 2022-02-26 /pmc/articles/PMC8882280/ /pubmed/35219305 http://dx.doi.org/10.1186/s12885-022-09330-9 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Wang, Xue
Wang, Ying
Xie, Fang
Song, Zi-Tian
Zhang, Zi-Qian
Zhao, Yun
Wang, Shi-Da
Hu, Hui
Zhang, Yan-Shu
Qian, Ling-Jia
Norepinephrine promotes glioma cell migration through up-regulating the expression of Twist1
title Norepinephrine promotes glioma cell migration through up-regulating the expression of Twist1
title_full Norepinephrine promotes glioma cell migration through up-regulating the expression of Twist1
title_fullStr Norepinephrine promotes glioma cell migration through up-regulating the expression of Twist1
title_full_unstemmed Norepinephrine promotes glioma cell migration through up-regulating the expression of Twist1
title_short Norepinephrine promotes glioma cell migration through up-regulating the expression of Twist1
title_sort norepinephrine promotes glioma cell migration through up-regulating the expression of twist1
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8882280/
https://www.ncbi.nlm.nih.gov/pubmed/35219305
http://dx.doi.org/10.1186/s12885-022-09330-9
work_keys_str_mv AT wangxue norepinephrinepromotesgliomacellmigrationthroughupregulatingtheexpressionoftwist1
AT wangying norepinephrinepromotesgliomacellmigrationthroughupregulatingtheexpressionoftwist1
AT xiefang norepinephrinepromotesgliomacellmigrationthroughupregulatingtheexpressionoftwist1
AT songzitian norepinephrinepromotesgliomacellmigrationthroughupregulatingtheexpressionoftwist1
AT zhangziqian norepinephrinepromotesgliomacellmigrationthroughupregulatingtheexpressionoftwist1
AT zhaoyun norepinephrinepromotesgliomacellmigrationthroughupregulatingtheexpressionoftwist1
AT wangshida norepinephrinepromotesgliomacellmigrationthroughupregulatingtheexpressionoftwist1
AT huhui norepinephrinepromotesgliomacellmigrationthroughupregulatingtheexpressionoftwist1
AT zhangyanshu norepinephrinepromotesgliomacellmigrationthroughupregulatingtheexpressionoftwist1
AT qianlingjia norepinephrinepromotesgliomacellmigrationthroughupregulatingtheexpressionoftwist1