SARS-CoV-2 proteome microarray for COVID-19 patient sera profiling

The immunogenicity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) proteome is largely unknown. Here we describe a protocol for analyzing sera samples with SARS-CoV-2 proteome microarray. The proteins were expressed by either E. coli expression system or eukaryotic cell expression sy...

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Autores principales: Lai, Dan-yun, Jiang, He-wei, Li, Yang, Zhang, Hai-nan, Tao, Sheng-ce
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8882418/
https://www.ncbi.nlm.nih.gov/pubmed/35310073
http://dx.doi.org/10.1016/j.xpro.2022.101238
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author Lai, Dan-yun
Jiang, He-wei
Li, Yang
Zhang, Hai-nan
Tao, Sheng-ce
author_facet Lai, Dan-yun
Jiang, He-wei
Li, Yang
Zhang, Hai-nan
Tao, Sheng-ce
author_sort Lai, Dan-yun
collection PubMed
description The immunogenicity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) proteome is largely unknown. Here we describe a protocol for analyzing sera samples with SARS-CoV-2 proteome microarray. The proteins were expressed by either E. coli expression system or eukaryotic cell expression systems and obtained by affinity purification. The protocol includes microarray fabricating and sera profiling, which will be used to build an antibody response landscape for IgG and IgM. The protocol may help to facilitate a deeper understanding of immunity related to SARS-CoV-2. For complete details on the use and execution of this protocol, please refer to Li et al. (2021c).
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spelling pubmed-88824182022-02-28 SARS-CoV-2 proteome microarray for COVID-19 patient sera profiling Lai, Dan-yun Jiang, He-wei Li, Yang Zhang, Hai-nan Tao, Sheng-ce STAR Protoc Protocol The immunogenicity of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) proteome is largely unknown. Here we describe a protocol for analyzing sera samples with SARS-CoV-2 proteome microarray. The proteins were expressed by either E. coli expression system or eukaryotic cell expression systems and obtained by affinity purification. The protocol includes microarray fabricating and sera profiling, which will be used to build an antibody response landscape for IgG and IgM. The protocol may help to facilitate a deeper understanding of immunity related to SARS-CoV-2. For complete details on the use and execution of this protocol, please refer to Li et al. (2021c). Elsevier 2022-02-28 /pmc/articles/PMC8882418/ /pubmed/35310073 http://dx.doi.org/10.1016/j.xpro.2022.101238 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Lai, Dan-yun
Jiang, He-wei
Li, Yang
Zhang, Hai-nan
Tao, Sheng-ce
SARS-CoV-2 proteome microarray for COVID-19 patient sera profiling
title SARS-CoV-2 proteome microarray for COVID-19 patient sera profiling
title_full SARS-CoV-2 proteome microarray for COVID-19 patient sera profiling
title_fullStr SARS-CoV-2 proteome microarray for COVID-19 patient sera profiling
title_full_unstemmed SARS-CoV-2 proteome microarray for COVID-19 patient sera profiling
title_short SARS-CoV-2 proteome microarray for COVID-19 patient sera profiling
title_sort sars-cov-2 proteome microarray for covid-19 patient sera profiling
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8882418/
https://www.ncbi.nlm.nih.gov/pubmed/35310073
http://dx.doi.org/10.1016/j.xpro.2022.101238
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