Cargando…

Toll-Like Receptor Evolution: Does Temperature Matter?

Toll-like receptors (TLRs) recognize conserved pathogen-associated molecular patterns (PAMPs) and are an ancient and well-conserved group of pattern recognition receptors (PRRs). The isolation of the Antarctic continent and its unique teleost fish and microbiota prompted the present investigation in...

Descripción completa

Detalles Bibliográficos
Autores principales: Sousa, Cármen, Fernandes, Stefan A., Cardoso, João C. R., Wang, Ying, Zhai, Wanying, Guerreiro, Pedro M., Chen, Liangbiao, Canário, Adelino V. M., Power, Deborah M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8882821/
https://www.ncbi.nlm.nih.gov/pubmed/35237266
http://dx.doi.org/10.3389/fimmu.2022.812890
_version_ 1784659780642537472
author Sousa, Cármen
Fernandes, Stefan A.
Cardoso, João C. R.
Wang, Ying
Zhai, Wanying
Guerreiro, Pedro M.
Chen, Liangbiao
Canário, Adelino V. M.
Power, Deborah M.
author_facet Sousa, Cármen
Fernandes, Stefan A.
Cardoso, João C. R.
Wang, Ying
Zhai, Wanying
Guerreiro, Pedro M.
Chen, Liangbiao
Canário, Adelino V. M.
Power, Deborah M.
author_sort Sousa, Cármen
collection PubMed
description Toll-like receptors (TLRs) recognize conserved pathogen-associated molecular patterns (PAMPs) and are an ancient and well-conserved group of pattern recognition receptors (PRRs). The isolation of the Antarctic continent and its unique teleost fish and microbiota prompted the present investigation into Tlr evolution. Gene homologues of tlr members in teleosts from temperate regions were present in the genome of Antarctic Nototheniidae and the non-Antarctic sister lineage Bovichtidae. Overall, in Nototheniidae apart from D. mawsoni, no major tlr gene family expansion or contraction occurred. Instead, lineage and species-specific changes in the ectodomain and LRR of Tlrs occurred, particularly in the Tlr11 superfamily that is well represented in fish. Positive selective pressure and associated sequence modifications in the TLR ectodomain and within the leucine-rich repeats (LRR), important for pathogen recognition, occurred in Tlr5, Tlr8, Tlr13, Tlr21, Tlr22, and Tlr23 presumably associated with the unique Antarctic microbiota. Exposure to lipopolysaccharide (Escherichia coli O111:B4) Gram negative bacteria did not modify tlr gene expression in N. rossii head–kidney or anterior intestine, although increased water temperature (+4°C) had a significant effect.
format Online
Article
Text
id pubmed-8882821
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-88828212022-03-01 Toll-Like Receptor Evolution: Does Temperature Matter? Sousa, Cármen Fernandes, Stefan A. Cardoso, João C. R. Wang, Ying Zhai, Wanying Guerreiro, Pedro M. Chen, Liangbiao Canário, Adelino V. M. Power, Deborah M. Front Immunol Immunology Toll-like receptors (TLRs) recognize conserved pathogen-associated molecular patterns (PAMPs) and are an ancient and well-conserved group of pattern recognition receptors (PRRs). The isolation of the Antarctic continent and its unique teleost fish and microbiota prompted the present investigation into Tlr evolution. Gene homologues of tlr members in teleosts from temperate regions were present in the genome of Antarctic Nototheniidae and the non-Antarctic sister lineage Bovichtidae. Overall, in Nototheniidae apart from D. mawsoni, no major tlr gene family expansion or contraction occurred. Instead, lineage and species-specific changes in the ectodomain and LRR of Tlrs occurred, particularly in the Tlr11 superfamily that is well represented in fish. Positive selective pressure and associated sequence modifications in the TLR ectodomain and within the leucine-rich repeats (LRR), important for pathogen recognition, occurred in Tlr5, Tlr8, Tlr13, Tlr21, Tlr22, and Tlr23 presumably associated with the unique Antarctic microbiota. Exposure to lipopolysaccharide (Escherichia coli O111:B4) Gram negative bacteria did not modify tlr gene expression in N. rossii head–kidney or anterior intestine, although increased water temperature (+4°C) had a significant effect. Frontiers Media S.A. 2022-02-14 /pmc/articles/PMC8882821/ /pubmed/35237266 http://dx.doi.org/10.3389/fimmu.2022.812890 Text en Copyright © 2022 Sousa, Fernandes, Cardoso, Wang, Zhai, Guerreiro, Chen, Canário and Power https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Sousa, Cármen
Fernandes, Stefan A.
Cardoso, João C. R.
Wang, Ying
Zhai, Wanying
Guerreiro, Pedro M.
Chen, Liangbiao
Canário, Adelino V. M.
Power, Deborah M.
Toll-Like Receptor Evolution: Does Temperature Matter?
title Toll-Like Receptor Evolution: Does Temperature Matter?
title_full Toll-Like Receptor Evolution: Does Temperature Matter?
title_fullStr Toll-Like Receptor Evolution: Does Temperature Matter?
title_full_unstemmed Toll-Like Receptor Evolution: Does Temperature Matter?
title_short Toll-Like Receptor Evolution: Does Temperature Matter?
title_sort toll-like receptor evolution: does temperature matter?
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8882821/
https://www.ncbi.nlm.nih.gov/pubmed/35237266
http://dx.doi.org/10.3389/fimmu.2022.812890
work_keys_str_mv AT sousacarmen tolllikereceptorevolutiondoestemperaturematter
AT fernandesstefana tolllikereceptorevolutiondoestemperaturematter
AT cardosojoaocr tolllikereceptorevolutiondoestemperaturematter
AT wangying tolllikereceptorevolutiondoestemperaturematter
AT zhaiwanying tolllikereceptorevolutiondoestemperaturematter
AT guerreiropedrom tolllikereceptorevolutiondoestemperaturematter
AT chenliangbiao tolllikereceptorevolutiondoestemperaturematter
AT canarioadelinovm tolllikereceptorevolutiondoestemperaturematter
AT powerdeborahm tolllikereceptorevolutiondoestemperaturematter