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CRISPR-Cas13d Exhibits Robust Antiviral Activity Against Seneca Valley Virus
In recent years, Seneca Valley virus (SVV) as a newly identified pathogen of porcine vesicular disease spread quickly and has posed a potential threat to the swine industry in several countries resulting in economic losses. Considering the evolution of SVV, attention should be given to controlling S...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8882862/ https://www.ncbi.nlm.nih.gov/pubmed/35237251 http://dx.doi.org/10.3389/fmicb.2022.835040 |
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author | Zhang, Yu-Yuan Sun, Ming-Xia Lian, Yuexiao Wang, Tong-Yun Jia, Mei-Yu Leng, Chaoliang Chen, Meng Bai, Yuan-Zhe Meng, Fandan Cai, Xue-Hui Tang, Yan-Dong |
author_facet | Zhang, Yu-Yuan Sun, Ming-Xia Lian, Yuexiao Wang, Tong-Yun Jia, Mei-Yu Leng, Chaoliang Chen, Meng Bai, Yuan-Zhe Meng, Fandan Cai, Xue-Hui Tang, Yan-Dong |
author_sort | Zhang, Yu-Yuan |
collection | PubMed |
description | In recent years, Seneca Valley virus (SVV) as a newly identified pathogen of porcine vesicular disease spread quickly and has posed a potential threat to the swine industry in several countries resulting in economic losses. Considering the evolution of SVV, attention should be given to controlling SVV epidemics. So far there are no commercial vaccines or drugs available to combat SVV. Therefore, development of strategies for preventing and controlling SVV infection should be taken into account. In the current study, we evaluated whether the CRISPR-Cas13d system could be used as a powerful tool against SVV infection. Besides, selected crRNAs showed different capacity against SVV infection. Our study suggests the CRISPR-Cas13d system significantly inhibited SVV replication and exhibited potent anti-SVV activity. This knowledge may provide a novel alternative strategy to control epidemics of SVV in the future. |
format | Online Article Text |
id | pubmed-8882862 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88828622022-03-01 CRISPR-Cas13d Exhibits Robust Antiviral Activity Against Seneca Valley Virus Zhang, Yu-Yuan Sun, Ming-Xia Lian, Yuexiao Wang, Tong-Yun Jia, Mei-Yu Leng, Chaoliang Chen, Meng Bai, Yuan-Zhe Meng, Fandan Cai, Xue-Hui Tang, Yan-Dong Front Microbiol Microbiology In recent years, Seneca Valley virus (SVV) as a newly identified pathogen of porcine vesicular disease spread quickly and has posed a potential threat to the swine industry in several countries resulting in economic losses. Considering the evolution of SVV, attention should be given to controlling SVV epidemics. So far there are no commercial vaccines or drugs available to combat SVV. Therefore, development of strategies for preventing and controlling SVV infection should be taken into account. In the current study, we evaluated whether the CRISPR-Cas13d system could be used as a powerful tool against SVV infection. Besides, selected crRNAs showed different capacity against SVV infection. Our study suggests the CRISPR-Cas13d system significantly inhibited SVV replication and exhibited potent anti-SVV activity. This knowledge may provide a novel alternative strategy to control epidemics of SVV in the future. Frontiers Media S.A. 2022-02-14 /pmc/articles/PMC8882862/ /pubmed/35237251 http://dx.doi.org/10.3389/fmicb.2022.835040 Text en Copyright © 2022 Zhang, Sun, Lian, Wang, Jia, Leng, Chen, Bai, Meng, Cai and Tang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Zhang, Yu-Yuan Sun, Ming-Xia Lian, Yuexiao Wang, Tong-Yun Jia, Mei-Yu Leng, Chaoliang Chen, Meng Bai, Yuan-Zhe Meng, Fandan Cai, Xue-Hui Tang, Yan-Dong CRISPR-Cas13d Exhibits Robust Antiviral Activity Against Seneca Valley Virus |
title | CRISPR-Cas13d Exhibits Robust Antiviral Activity Against Seneca Valley Virus |
title_full | CRISPR-Cas13d Exhibits Robust Antiviral Activity Against Seneca Valley Virus |
title_fullStr | CRISPR-Cas13d Exhibits Robust Antiviral Activity Against Seneca Valley Virus |
title_full_unstemmed | CRISPR-Cas13d Exhibits Robust Antiviral Activity Against Seneca Valley Virus |
title_short | CRISPR-Cas13d Exhibits Robust Antiviral Activity Against Seneca Valley Virus |
title_sort | crispr-cas13d exhibits robust antiviral activity against seneca valley virus |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8882862/ https://www.ncbi.nlm.nih.gov/pubmed/35237251 http://dx.doi.org/10.3389/fmicb.2022.835040 |
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