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Desiccation as a Post-maturation Treatment Helps Complete Maturation of Norway Spruce Somatic Embryos: Carbohydrates, Phytohormones and Proteomic Status

Exposure of Norway spruce (Picea abies) somatic embryos and those of many other conifers to post-maturation desiccation treatment significantly improves their germination. An integration analysis was conducted to understand the underlying processes induced during the desiccation phase at the molecul...

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Autores principales: Eliášová, Kateřina, Konrádová, Hana, Dobrev, Petre I., Motyka, Václav, Lomenech, Anne-Marie, Fischerová, Lucie, Lelu-Walter, Marie-Anne, Vondráková, Zuzana, Teyssier, Caroline
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8882965/
https://www.ncbi.nlm.nih.gov/pubmed/35237290
http://dx.doi.org/10.3389/fpls.2022.823617
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author Eliášová, Kateřina
Konrádová, Hana
Dobrev, Petre I.
Motyka, Václav
Lomenech, Anne-Marie
Fischerová, Lucie
Lelu-Walter, Marie-Anne
Vondráková, Zuzana
Teyssier, Caroline
author_facet Eliášová, Kateřina
Konrádová, Hana
Dobrev, Petre I.
Motyka, Václav
Lomenech, Anne-Marie
Fischerová, Lucie
Lelu-Walter, Marie-Anne
Vondráková, Zuzana
Teyssier, Caroline
author_sort Eliášová, Kateřina
collection PubMed
description Exposure of Norway spruce (Picea abies) somatic embryos and those of many other conifers to post-maturation desiccation treatment significantly improves their germination. An integration analysis was conducted to understand the underlying processes induced during the desiccation phase at the molecular level. Carbohydrate, protein and phytohormone assays associated with histological and proteomic studies were performed for the evaluation of markers and actors in this phase. Multivariate comparison of mature somatic embryos with mature desiccated somatic embryos and/or zygotic embryos provided new insights into the processes involved during the desiccation step of somatic embryogenesis. Desiccated embryos were characterized by reduced levels of starch and soluble carbohydrates but elevated levels of raffinose family oligosaccharides. Desiccation treatment decreased the content of abscisic acid and its derivatives but increased total auxins and cytokinins. The content of phytohormones in dry zygotic embryos was lower than in somatic embryos, but their profile was mostly analogous, apart from differences in cytokinin profiles. The biological processes “Acquisition of desiccation tolerance”, “Response to stimulus”, “Response to stress” and “Stored energy” were activated in both the desiccated somatic embryos and zygotic embryos when compared to the proteome of mature somatic embryos before desiccation. Based on the specific biochemical changes of important constituents (abscisic acid, raffinose, stachyose, LEA proteins and cruciferins) induced by the desiccation treatment and observed similarities between somatic and zygotic P. abies embryos, we concluded that the somatic embryos approximated to a state of desiccation tolerance. This physiological change could be responsible for the reorientation of Norway spruce somatic embryos toward a stage suitable for germination.
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spelling pubmed-88829652022-03-01 Desiccation as a Post-maturation Treatment Helps Complete Maturation of Norway Spruce Somatic Embryos: Carbohydrates, Phytohormones and Proteomic Status Eliášová, Kateřina Konrádová, Hana Dobrev, Petre I. Motyka, Václav Lomenech, Anne-Marie Fischerová, Lucie Lelu-Walter, Marie-Anne Vondráková, Zuzana Teyssier, Caroline Front Plant Sci Plant Science Exposure of Norway spruce (Picea abies) somatic embryos and those of many other conifers to post-maturation desiccation treatment significantly improves their germination. An integration analysis was conducted to understand the underlying processes induced during the desiccation phase at the molecular level. Carbohydrate, protein and phytohormone assays associated with histological and proteomic studies were performed for the evaluation of markers and actors in this phase. Multivariate comparison of mature somatic embryos with mature desiccated somatic embryos and/or zygotic embryos provided new insights into the processes involved during the desiccation step of somatic embryogenesis. Desiccated embryos were characterized by reduced levels of starch and soluble carbohydrates but elevated levels of raffinose family oligosaccharides. Desiccation treatment decreased the content of abscisic acid and its derivatives but increased total auxins and cytokinins. The content of phytohormones in dry zygotic embryos was lower than in somatic embryos, but their profile was mostly analogous, apart from differences in cytokinin profiles. The biological processes “Acquisition of desiccation tolerance”, “Response to stimulus”, “Response to stress” and “Stored energy” were activated in both the desiccated somatic embryos and zygotic embryos when compared to the proteome of mature somatic embryos before desiccation. Based on the specific biochemical changes of important constituents (abscisic acid, raffinose, stachyose, LEA proteins and cruciferins) induced by the desiccation treatment and observed similarities between somatic and zygotic P. abies embryos, we concluded that the somatic embryos approximated to a state of desiccation tolerance. This physiological change could be responsible for the reorientation of Norway spruce somatic embryos toward a stage suitable for germination. Frontiers Media S.A. 2022-02-14 /pmc/articles/PMC8882965/ /pubmed/35237290 http://dx.doi.org/10.3389/fpls.2022.823617 Text en Copyright © 2022 Eliášová, Konrádová, Dobrev, Motyka, Lomenech, Fischerová, Lelu-Walter, Vondráková and Teyssier. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Eliášová, Kateřina
Konrádová, Hana
Dobrev, Petre I.
Motyka, Václav
Lomenech, Anne-Marie
Fischerová, Lucie
Lelu-Walter, Marie-Anne
Vondráková, Zuzana
Teyssier, Caroline
Desiccation as a Post-maturation Treatment Helps Complete Maturation of Norway Spruce Somatic Embryos: Carbohydrates, Phytohormones and Proteomic Status
title Desiccation as a Post-maturation Treatment Helps Complete Maturation of Norway Spruce Somatic Embryos: Carbohydrates, Phytohormones and Proteomic Status
title_full Desiccation as a Post-maturation Treatment Helps Complete Maturation of Norway Spruce Somatic Embryos: Carbohydrates, Phytohormones and Proteomic Status
title_fullStr Desiccation as a Post-maturation Treatment Helps Complete Maturation of Norway Spruce Somatic Embryos: Carbohydrates, Phytohormones and Proteomic Status
title_full_unstemmed Desiccation as a Post-maturation Treatment Helps Complete Maturation of Norway Spruce Somatic Embryos: Carbohydrates, Phytohormones and Proteomic Status
title_short Desiccation as a Post-maturation Treatment Helps Complete Maturation of Norway Spruce Somatic Embryos: Carbohydrates, Phytohormones and Proteomic Status
title_sort desiccation as a post-maturation treatment helps complete maturation of norway spruce somatic embryos: carbohydrates, phytohormones and proteomic status
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8882965/
https://www.ncbi.nlm.nih.gov/pubmed/35237290
http://dx.doi.org/10.3389/fpls.2022.823617
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