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The Transcription Factor Lrp of Pantoea stewartii subsp. stewartii Controls Capsule Production, Motility, and Virulence Important for in planta Growth

The bacterial phytopathogen Pantoea stewartii subsp. stewartii causes leaf blight and Stewart’s wilt disease in susceptible corn varieties. A previous RNA-Seq study examined P. stewartii gene expression patterns during late-stage infection in the xylem, and a Tn-Seq study using a P. stewartii mutant...

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Autores principales: Bartholomew, Holly P., Reynoso, Guadalupe, Thomas, Brandi J., Mullins, Chase M., Smith, Chastyn, Gentzel, Irene N., Giese, Laura A., Mackey, David, Stevens, Ann M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8882988/
https://www.ncbi.nlm.nih.gov/pubmed/35237242
http://dx.doi.org/10.3389/fmicb.2021.806504
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author Bartholomew, Holly P.
Reynoso, Guadalupe
Thomas, Brandi J.
Mullins, Chase M.
Smith, Chastyn
Gentzel, Irene N.
Giese, Laura A.
Mackey, David
Stevens, Ann M.
author_facet Bartholomew, Holly P.
Reynoso, Guadalupe
Thomas, Brandi J.
Mullins, Chase M.
Smith, Chastyn
Gentzel, Irene N.
Giese, Laura A.
Mackey, David
Stevens, Ann M.
author_sort Bartholomew, Holly P.
collection PubMed
description The bacterial phytopathogen Pantoea stewartii subsp. stewartii causes leaf blight and Stewart’s wilt disease in susceptible corn varieties. A previous RNA-Seq study examined P. stewartii gene expression patterns during late-stage infection in the xylem, and a Tn-Seq study using a P. stewartii mutant library revealed genes essential for colonization of the xylem. Based on these findings, strains with in-frame chromosomal deletions in the genes encoding seven transcription factors (NsrR, IscR, Nac, Lrp, DSJ_00125, DSJ_03645, and DSJ_18135) and one hypothetical protein (DSJ_21690) were constructed to further evaluate the role of the encoded gene products during in vitro and in planta growth. Assays for capsule production and motility indicate that Lrp plays a role in regulating these two key physiological outputs in vitro. Single infections of each deletion strain into the xylem of corn seedlings determined that Lrp plays a significant role in P. stewartii virulence. In planta xylem competition assays between co-inoculated deletion and the corresponding complementation or wild-type strains as well as in vitro growth curves determined that Lrp controls functions important for P. stewartii colonization and growth in corn plants, whereas IscR may have a more generalized impact on growth. Defining the role of essential transcription factors, such as Lrp, during in planta growth will enable modeling of key components of the P. stewartii regulatory network utilized during growth in corn plants.
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spelling pubmed-88829882022-03-01 The Transcription Factor Lrp of Pantoea stewartii subsp. stewartii Controls Capsule Production, Motility, and Virulence Important for in planta Growth Bartholomew, Holly P. Reynoso, Guadalupe Thomas, Brandi J. Mullins, Chase M. Smith, Chastyn Gentzel, Irene N. Giese, Laura A. Mackey, David Stevens, Ann M. Front Microbiol Microbiology The bacterial phytopathogen Pantoea stewartii subsp. stewartii causes leaf blight and Stewart’s wilt disease in susceptible corn varieties. A previous RNA-Seq study examined P. stewartii gene expression patterns during late-stage infection in the xylem, and a Tn-Seq study using a P. stewartii mutant library revealed genes essential for colonization of the xylem. Based on these findings, strains with in-frame chromosomal deletions in the genes encoding seven transcription factors (NsrR, IscR, Nac, Lrp, DSJ_00125, DSJ_03645, and DSJ_18135) and one hypothetical protein (DSJ_21690) were constructed to further evaluate the role of the encoded gene products during in vitro and in planta growth. Assays for capsule production and motility indicate that Lrp plays a role in regulating these two key physiological outputs in vitro. Single infections of each deletion strain into the xylem of corn seedlings determined that Lrp plays a significant role in P. stewartii virulence. In planta xylem competition assays between co-inoculated deletion and the corresponding complementation or wild-type strains as well as in vitro growth curves determined that Lrp controls functions important for P. stewartii colonization and growth in corn plants, whereas IscR may have a more generalized impact on growth. Defining the role of essential transcription factors, such as Lrp, during in planta growth will enable modeling of key components of the P. stewartii regulatory network utilized during growth in corn plants. Frontiers Media S.A. 2022-02-14 /pmc/articles/PMC8882988/ /pubmed/35237242 http://dx.doi.org/10.3389/fmicb.2021.806504 Text en Copyright © 2022 Bartholomew, Reynoso, Thomas, Mullins, Smith, Gentzel, Giese, Mackey and Stevens. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Bartholomew, Holly P.
Reynoso, Guadalupe
Thomas, Brandi J.
Mullins, Chase M.
Smith, Chastyn
Gentzel, Irene N.
Giese, Laura A.
Mackey, David
Stevens, Ann M.
The Transcription Factor Lrp of Pantoea stewartii subsp. stewartii Controls Capsule Production, Motility, and Virulence Important for in planta Growth
title The Transcription Factor Lrp of Pantoea stewartii subsp. stewartii Controls Capsule Production, Motility, and Virulence Important for in planta Growth
title_full The Transcription Factor Lrp of Pantoea stewartii subsp. stewartii Controls Capsule Production, Motility, and Virulence Important for in planta Growth
title_fullStr The Transcription Factor Lrp of Pantoea stewartii subsp. stewartii Controls Capsule Production, Motility, and Virulence Important for in planta Growth
title_full_unstemmed The Transcription Factor Lrp of Pantoea stewartii subsp. stewartii Controls Capsule Production, Motility, and Virulence Important for in planta Growth
title_short The Transcription Factor Lrp of Pantoea stewartii subsp. stewartii Controls Capsule Production, Motility, and Virulence Important for in planta Growth
title_sort transcription factor lrp of pantoea stewartii subsp. stewartii controls capsule production, motility, and virulence important for in planta growth
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8882988/
https://www.ncbi.nlm.nih.gov/pubmed/35237242
http://dx.doi.org/10.3389/fmicb.2021.806504
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