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Decontamination of High-Efficiency Mask Filters From Respiratory Pathogens Including SARS-CoV-2 by Non-thermal Plasma

The current pandemic resulted in a rapidly increasing demand for personal protective equipment (PPE) initially leading to severe shortages of these items. Hence, during an unexpected and fast virus spread, the possibility of reusing highly efficient protective equipment could provide a viable soluti...

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Autores principales: Obrová, Klára, Vaňková, Eva, Sláma, Michal, Hodek, Jan, Khun, Josef, Ulrychová, Lucie, Nogueira, Filomena, Laos, Triin, Sponseiler, Isabella, Kašparová, Petra, Machková, Anna, Weber, Jan, Scholtz, Vladimír, Lion, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8883054/
https://www.ncbi.nlm.nih.gov/pubmed/35237577
http://dx.doi.org/10.3389/fbioe.2022.815393
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author Obrová, Klára
Vaňková, Eva
Sláma, Michal
Hodek, Jan
Khun, Josef
Ulrychová, Lucie
Nogueira, Filomena
Laos, Triin
Sponseiler, Isabella
Kašparová, Petra
Machková, Anna
Weber, Jan
Scholtz, Vladimír
Lion, Thomas
author_facet Obrová, Klára
Vaňková, Eva
Sláma, Michal
Hodek, Jan
Khun, Josef
Ulrychová, Lucie
Nogueira, Filomena
Laos, Triin
Sponseiler, Isabella
Kašparová, Petra
Machková, Anna
Weber, Jan
Scholtz, Vladimír
Lion, Thomas
author_sort Obrová, Klára
collection PubMed
description The current pandemic resulted in a rapidly increasing demand for personal protective equipment (PPE) initially leading to severe shortages of these items. Hence, during an unexpected and fast virus spread, the possibility of reusing highly efficient protective equipment could provide a viable solution for keeping both healthcare professionals and the general public equipped and protected. This requires an efficient decontamination technique that preserves functionality of the sensitive materials used for PPE production. Non-thermal plasma (NTP) is a decontamination technique with documented efficiency against select bacterial and fungal pathogens combined with low damage to exposed materials. We have investigated NTP for decontamination of high-efficiency P3 R filters from viral respiratory pathogens in comparison to other commonly used techniques. We show that NTP treatment completely inactivates SARS-CoV-2 and three other common human respiratory viruses including Influenza A, Rhinovirus and Adenovirus, revealing an efficiency comparable to 90°C dry heat or UVC light. Unlike some of the tested techniques (e.g., autoclaving), NTP neither influenced the filtering efficiency nor the microstructure of the filter. We demonstrate that NTP is a powerful and economic technology for efficient decontamination of protective filters and other sensitive materials from different respiratory pathogens.
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spelling pubmed-88830542022-03-01 Decontamination of High-Efficiency Mask Filters From Respiratory Pathogens Including SARS-CoV-2 by Non-thermal Plasma Obrová, Klára Vaňková, Eva Sláma, Michal Hodek, Jan Khun, Josef Ulrychová, Lucie Nogueira, Filomena Laos, Triin Sponseiler, Isabella Kašparová, Petra Machková, Anna Weber, Jan Scholtz, Vladimír Lion, Thomas Front Bioeng Biotechnol Bioengineering and Biotechnology The current pandemic resulted in a rapidly increasing demand for personal protective equipment (PPE) initially leading to severe shortages of these items. Hence, during an unexpected and fast virus spread, the possibility of reusing highly efficient protective equipment could provide a viable solution for keeping both healthcare professionals and the general public equipped and protected. This requires an efficient decontamination technique that preserves functionality of the sensitive materials used for PPE production. Non-thermal plasma (NTP) is a decontamination technique with documented efficiency against select bacterial and fungal pathogens combined with low damage to exposed materials. We have investigated NTP for decontamination of high-efficiency P3 R filters from viral respiratory pathogens in comparison to other commonly used techniques. We show that NTP treatment completely inactivates SARS-CoV-2 and three other common human respiratory viruses including Influenza A, Rhinovirus and Adenovirus, revealing an efficiency comparable to 90°C dry heat or UVC light. Unlike some of the tested techniques (e.g., autoclaving), NTP neither influenced the filtering efficiency nor the microstructure of the filter. We demonstrate that NTP is a powerful and economic technology for efficient decontamination of protective filters and other sensitive materials from different respiratory pathogens. Frontiers Media S.A. 2022-02-14 /pmc/articles/PMC8883054/ /pubmed/35237577 http://dx.doi.org/10.3389/fbioe.2022.815393 Text en Copyright © 2022 Obrová, Vaňková, Sláma, Hodek, Khun, Ulrychová, Nogueira, Laos, Sponseiler, Kašparová, Machková, Weber, Scholtz and Lion. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Obrová, Klára
Vaňková, Eva
Sláma, Michal
Hodek, Jan
Khun, Josef
Ulrychová, Lucie
Nogueira, Filomena
Laos, Triin
Sponseiler, Isabella
Kašparová, Petra
Machková, Anna
Weber, Jan
Scholtz, Vladimír
Lion, Thomas
Decontamination of High-Efficiency Mask Filters From Respiratory Pathogens Including SARS-CoV-2 by Non-thermal Plasma
title Decontamination of High-Efficiency Mask Filters From Respiratory Pathogens Including SARS-CoV-2 by Non-thermal Plasma
title_full Decontamination of High-Efficiency Mask Filters From Respiratory Pathogens Including SARS-CoV-2 by Non-thermal Plasma
title_fullStr Decontamination of High-Efficiency Mask Filters From Respiratory Pathogens Including SARS-CoV-2 by Non-thermal Plasma
title_full_unstemmed Decontamination of High-Efficiency Mask Filters From Respiratory Pathogens Including SARS-CoV-2 by Non-thermal Plasma
title_short Decontamination of High-Efficiency Mask Filters From Respiratory Pathogens Including SARS-CoV-2 by Non-thermal Plasma
title_sort decontamination of high-efficiency mask filters from respiratory pathogens including sars-cov-2 by non-thermal plasma
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8883054/
https://www.ncbi.nlm.nih.gov/pubmed/35237577
http://dx.doi.org/10.3389/fbioe.2022.815393
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