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Anti-SARS-CoV-2 Omicron Antibodies Isolated from a SARS-CoV-2 Delta Semi-Immune Phage Display Library

This report describes the discovery and characterization of antibodies with potential broad SARS-CoV-2 neutralization profiles. The antibodies were obtained from a phage display library built with the VH repertoire of a convalescent COVID-19 patient who was infected with SARS-CoV-2 B.1.617.2 (Delta)...

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Autores principales: Mendoza-Salazar, Ivette, Gómez-Castellano, Keyla M., González-González, Edith, Gamboa-Suasnavart, Ramsés, Rodríguez-Luna, Stefany D., Santiago-Casas, Giovanni, Cortés-Paniagua, María I., Pérez-Tapia, Sonia M., Almagro, Juan C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8883901/
https://www.ncbi.nlm.nih.gov/pubmed/35225871
http://dx.doi.org/10.3390/antib11010013
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author Mendoza-Salazar, Ivette
Gómez-Castellano, Keyla M.
González-González, Edith
Gamboa-Suasnavart, Ramsés
Rodríguez-Luna, Stefany D.
Santiago-Casas, Giovanni
Cortés-Paniagua, María I.
Pérez-Tapia, Sonia M.
Almagro, Juan C.
author_facet Mendoza-Salazar, Ivette
Gómez-Castellano, Keyla M.
González-González, Edith
Gamboa-Suasnavart, Ramsés
Rodríguez-Luna, Stefany D.
Santiago-Casas, Giovanni
Cortés-Paniagua, María I.
Pérez-Tapia, Sonia M.
Almagro, Juan C.
author_sort Mendoza-Salazar, Ivette
collection PubMed
description This report describes the discovery and characterization of antibodies with potential broad SARS-CoV-2 neutralization profiles. The antibodies were obtained from a phage display library built with the VH repertoire of a convalescent COVID-19 patient who was infected with SARS-CoV-2 B.1.617.2 (Delta). The patient received a single dose of Ad5-nCoV vaccine (Convidecia™, CanSino Biologics Inc.) one month before developing COVID-19 symptoms. Four synthetic VL libraries were used as counterparts of the immune VH repertoire. After three rounds of panning with SARS-CoV-2 receptor-binding domain wildtype (RBD-WT) 34 unique scFvs, were identified, with 27 cross-reactive for the RBD-WT and RBD Delta (RBD-DT), and seven specifics for the RBD-WT. The cross-reactive scFvs were more diverse than the RBD-WT specific ones, being encoded by several IGHV genes from the IGHV1 and IGHV3 families combined with short HCDR3s. Six cross-reactive scFvs and one RBD-WT specific scFv were converted to human IgG1 (hIgG1). Out of the seven antibodies, six blocked the RBD-WT binding to angiotensin converting enzyme 2 (ACE2), suggesting these antibodies may neutralize the SARS-CoV-2 infection. Importantly, one of the antibodies also recognized the RBD from the B.1.1.529 (Omicron) isolate, implying that the VH repertoire of the convalescent patient would protect against SARS-CoV-2 Wildtype, Delta, and Omicron. From a practical viewpoint, the triple cross-reactive antibody provides the substrate for developing therapeutic antibodies with a broad SARS-CoV-2 neutralization profile.
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spelling pubmed-88839012022-03-01 Anti-SARS-CoV-2 Omicron Antibodies Isolated from a SARS-CoV-2 Delta Semi-Immune Phage Display Library Mendoza-Salazar, Ivette Gómez-Castellano, Keyla M. González-González, Edith Gamboa-Suasnavart, Ramsés Rodríguez-Luna, Stefany D. Santiago-Casas, Giovanni Cortés-Paniagua, María I. Pérez-Tapia, Sonia M. Almagro, Juan C. Antibodies (Basel) Article This report describes the discovery and characterization of antibodies with potential broad SARS-CoV-2 neutralization profiles. The antibodies were obtained from a phage display library built with the VH repertoire of a convalescent COVID-19 patient who was infected with SARS-CoV-2 B.1.617.2 (Delta). The patient received a single dose of Ad5-nCoV vaccine (Convidecia™, CanSino Biologics Inc.) one month before developing COVID-19 symptoms. Four synthetic VL libraries were used as counterparts of the immune VH repertoire. After three rounds of panning with SARS-CoV-2 receptor-binding domain wildtype (RBD-WT) 34 unique scFvs, were identified, with 27 cross-reactive for the RBD-WT and RBD Delta (RBD-DT), and seven specifics for the RBD-WT. The cross-reactive scFvs were more diverse than the RBD-WT specific ones, being encoded by several IGHV genes from the IGHV1 and IGHV3 families combined with short HCDR3s. Six cross-reactive scFvs and one RBD-WT specific scFv were converted to human IgG1 (hIgG1). Out of the seven antibodies, six blocked the RBD-WT binding to angiotensin converting enzyme 2 (ACE2), suggesting these antibodies may neutralize the SARS-CoV-2 infection. Importantly, one of the antibodies also recognized the RBD from the B.1.1.529 (Omicron) isolate, implying that the VH repertoire of the convalescent patient would protect against SARS-CoV-2 Wildtype, Delta, and Omicron. From a practical viewpoint, the triple cross-reactive antibody provides the substrate for developing therapeutic antibodies with a broad SARS-CoV-2 neutralization profile. MDPI 2022-02-10 /pmc/articles/PMC8883901/ /pubmed/35225871 http://dx.doi.org/10.3390/antib11010013 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mendoza-Salazar, Ivette
Gómez-Castellano, Keyla M.
González-González, Edith
Gamboa-Suasnavart, Ramsés
Rodríguez-Luna, Stefany D.
Santiago-Casas, Giovanni
Cortés-Paniagua, María I.
Pérez-Tapia, Sonia M.
Almagro, Juan C.
Anti-SARS-CoV-2 Omicron Antibodies Isolated from a SARS-CoV-2 Delta Semi-Immune Phage Display Library
title Anti-SARS-CoV-2 Omicron Antibodies Isolated from a SARS-CoV-2 Delta Semi-Immune Phage Display Library
title_full Anti-SARS-CoV-2 Omicron Antibodies Isolated from a SARS-CoV-2 Delta Semi-Immune Phage Display Library
title_fullStr Anti-SARS-CoV-2 Omicron Antibodies Isolated from a SARS-CoV-2 Delta Semi-Immune Phage Display Library
title_full_unstemmed Anti-SARS-CoV-2 Omicron Antibodies Isolated from a SARS-CoV-2 Delta Semi-Immune Phage Display Library
title_short Anti-SARS-CoV-2 Omicron Antibodies Isolated from a SARS-CoV-2 Delta Semi-Immune Phage Display Library
title_sort anti-sars-cov-2 omicron antibodies isolated from a sars-cov-2 delta semi-immune phage display library
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8883901/
https://www.ncbi.nlm.nih.gov/pubmed/35225871
http://dx.doi.org/10.3390/antib11010013
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