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In Vitro Study of the Recruitment and Expansion of Mesenchymal Stem Cells at the Interface of a Cu-Doped PCL-Bioglass Scaffold

Developing new barrier membranes with improved biomechanical characteristics has acquired much interest owing to their crucial role in the field of periodontal tissue regeneration. In this regard, we enriched the electrospun polycaprolactone (PCL)/gelatin (Gel) membranes by adding bioglass (BG) or C...

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Autores principales: Malekahmadi, Behnaz, Esfahanian, Vahid, Ejeian, Fatemeh, Dastgurdi, Maziar Ebrahimi, Agheb, Maria, Kaveian, Faranak, Rafienia, Mohammad, Nasr-Esfahani, Mohammad Hossein
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8883986/
https://www.ncbi.nlm.nih.gov/pubmed/35225912
http://dx.doi.org/10.3390/biomimetics7010019
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author Malekahmadi, Behnaz
Esfahanian, Vahid
Ejeian, Fatemeh
Dastgurdi, Maziar Ebrahimi
Agheb, Maria
Kaveian, Faranak
Rafienia, Mohammad
Nasr-Esfahani, Mohammad Hossein
author_facet Malekahmadi, Behnaz
Esfahanian, Vahid
Ejeian, Fatemeh
Dastgurdi, Maziar Ebrahimi
Agheb, Maria
Kaveian, Faranak
Rafienia, Mohammad
Nasr-Esfahani, Mohammad Hossein
author_sort Malekahmadi, Behnaz
collection PubMed
description Developing new barrier membranes with improved biomechanical characteristics has acquired much interest owing to their crucial role in the field of periodontal tissue regeneration. In this regard, we enriched the electrospun polycaprolactone (PCL)/gelatin (Gel) membranes by adding bioglass (BG) or Cu-doped bioglass (CuBG) and examined their cellular adhesion and proliferation potential in the presence of alveolar bone marrow-derived mesenchymal stem cells (aBMSCs). The membranes were fabricated and characterized using mechanical strength, SEM, FTIR, EDX, and ICP assay. Besides, aBMSCs were isolated, characterized, and seeded with a density of 35,000 cells in each experimental group. Next, the cellular morphology, cell adhesion capacity, proliferation rate, and membrane antibacterial activity were assessed. The results displayed a significant improvement in the wettability, pore size, and Young’s modulus of the PCL membrane following the incorporation of gelatin and CuBG particles. Moreover, all scaffolds exhibited reasonable biocompatibility and bioactivity in physiological conditions. Although the PCL/Gel/CuBG membrane revealed the lowest primary cell attachment, cells were grown properly and reached the confluent state after seven days. In conclusion, we found a reasonable level of attachment and proliferation of aBMSCs on all modified membranes. Meanwhile, a trace amount of Cu provided superiority for PCL/Gel/CuBG in periodontal tissue regeneration.
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spelling pubmed-88839862022-03-01 In Vitro Study of the Recruitment and Expansion of Mesenchymal Stem Cells at the Interface of a Cu-Doped PCL-Bioglass Scaffold Malekahmadi, Behnaz Esfahanian, Vahid Ejeian, Fatemeh Dastgurdi, Maziar Ebrahimi Agheb, Maria Kaveian, Faranak Rafienia, Mohammad Nasr-Esfahani, Mohammad Hossein Biomimetics (Basel) Article Developing new barrier membranes with improved biomechanical characteristics has acquired much interest owing to their crucial role in the field of periodontal tissue regeneration. In this regard, we enriched the electrospun polycaprolactone (PCL)/gelatin (Gel) membranes by adding bioglass (BG) or Cu-doped bioglass (CuBG) and examined their cellular adhesion and proliferation potential in the presence of alveolar bone marrow-derived mesenchymal stem cells (aBMSCs). The membranes were fabricated and characterized using mechanical strength, SEM, FTIR, EDX, and ICP assay. Besides, aBMSCs were isolated, characterized, and seeded with a density of 35,000 cells in each experimental group. Next, the cellular morphology, cell adhesion capacity, proliferation rate, and membrane antibacterial activity were assessed. The results displayed a significant improvement in the wettability, pore size, and Young’s modulus of the PCL membrane following the incorporation of gelatin and CuBG particles. Moreover, all scaffolds exhibited reasonable biocompatibility and bioactivity in physiological conditions. Although the PCL/Gel/CuBG membrane revealed the lowest primary cell attachment, cells were grown properly and reached the confluent state after seven days. In conclusion, we found a reasonable level of attachment and proliferation of aBMSCs on all modified membranes. Meanwhile, a trace amount of Cu provided superiority for PCL/Gel/CuBG in periodontal tissue regeneration. MDPI 2022-01-21 /pmc/articles/PMC8883986/ /pubmed/35225912 http://dx.doi.org/10.3390/biomimetics7010019 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Malekahmadi, Behnaz
Esfahanian, Vahid
Ejeian, Fatemeh
Dastgurdi, Maziar Ebrahimi
Agheb, Maria
Kaveian, Faranak
Rafienia, Mohammad
Nasr-Esfahani, Mohammad Hossein
In Vitro Study of the Recruitment and Expansion of Mesenchymal Stem Cells at the Interface of a Cu-Doped PCL-Bioglass Scaffold
title In Vitro Study of the Recruitment and Expansion of Mesenchymal Stem Cells at the Interface of a Cu-Doped PCL-Bioglass Scaffold
title_full In Vitro Study of the Recruitment and Expansion of Mesenchymal Stem Cells at the Interface of a Cu-Doped PCL-Bioglass Scaffold
title_fullStr In Vitro Study of the Recruitment and Expansion of Mesenchymal Stem Cells at the Interface of a Cu-Doped PCL-Bioglass Scaffold
title_full_unstemmed In Vitro Study of the Recruitment and Expansion of Mesenchymal Stem Cells at the Interface of a Cu-Doped PCL-Bioglass Scaffold
title_short In Vitro Study of the Recruitment and Expansion of Mesenchymal Stem Cells at the Interface of a Cu-Doped PCL-Bioglass Scaffold
title_sort in vitro study of the recruitment and expansion of mesenchymal stem cells at the interface of a cu-doped pcl-bioglass scaffold
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8883986/
https://www.ncbi.nlm.nih.gov/pubmed/35225912
http://dx.doi.org/10.3390/biomimetics7010019
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