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Cadaverine Is a Switch in the Lysine Degradation Pathway in Pseudomonas aeruginosa Biofilm Identified by Untargeted Metabolomics
There is a critical need to accurately diagnose, prevent, and treat biofilms in humans. The biofilm forming P. aeruginosa bacteria can cause acute and chronic infections, which are difficult to treat due to their ability to evade host defenses along with an inherent antibiotic-tolerance. Using an un...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8884266/ https://www.ncbi.nlm.nih.gov/pubmed/35237533 http://dx.doi.org/10.3389/fcimb.2022.833269 |
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author | Leggett, Abigail Li, Da-Wei Sindeldecker, Devin Staats, Amelia Rigel, Nicholas Bruschweiler-Li, Lei Brüschweiler, Rafael Stoodley, Paul |
author_facet | Leggett, Abigail Li, Da-Wei Sindeldecker, Devin Staats, Amelia Rigel, Nicholas Bruschweiler-Li, Lei Brüschweiler, Rafael Stoodley, Paul |
author_sort | Leggett, Abigail |
collection | PubMed |
description | There is a critical need to accurately diagnose, prevent, and treat biofilms in humans. The biofilm forming P. aeruginosa bacteria can cause acute and chronic infections, which are difficult to treat due to their ability to evade host defenses along with an inherent antibiotic-tolerance. Using an untargeted NMR-based metabolomics approach, we identified statistically significant differences in 52 metabolites between P. aeruginosa grown in the planktonic and lawn biofilm states. Among them, the metabolites of the cadaverine branch of the lysine degradation pathway were systematically decreased in biofilm. Exogenous supplementation of cadaverine caused significantly increased planktonic growth, decreased biofilm accumulation by 49% and led to altered biofilm morphology, converting to a pellicle biofilm at the air-liquid interface. Our findings show how metabolic pathway differences directly affect the growth mode in P. aeruginosa and could support interventional strategies to control biofilm formation. |
format | Online Article Text |
id | pubmed-8884266 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-88842662022-03-01 Cadaverine Is a Switch in the Lysine Degradation Pathway in Pseudomonas aeruginosa Biofilm Identified by Untargeted Metabolomics Leggett, Abigail Li, Da-Wei Sindeldecker, Devin Staats, Amelia Rigel, Nicholas Bruschweiler-Li, Lei Brüschweiler, Rafael Stoodley, Paul Front Cell Infect Microbiol Cellular and Infection Microbiology There is a critical need to accurately diagnose, prevent, and treat biofilms in humans. The biofilm forming P. aeruginosa bacteria can cause acute and chronic infections, which are difficult to treat due to their ability to evade host defenses along with an inherent antibiotic-tolerance. Using an untargeted NMR-based metabolomics approach, we identified statistically significant differences in 52 metabolites between P. aeruginosa grown in the planktonic and lawn biofilm states. Among them, the metabolites of the cadaverine branch of the lysine degradation pathway were systematically decreased in biofilm. Exogenous supplementation of cadaverine caused significantly increased planktonic growth, decreased biofilm accumulation by 49% and led to altered biofilm morphology, converting to a pellicle biofilm at the air-liquid interface. Our findings show how metabolic pathway differences directly affect the growth mode in P. aeruginosa and could support interventional strategies to control biofilm formation. Frontiers Media S.A. 2022-02-14 /pmc/articles/PMC8884266/ /pubmed/35237533 http://dx.doi.org/10.3389/fcimb.2022.833269 Text en Copyright © 2022 Leggett, Li, Sindeldecker, Staats, Rigel, Bruschweiler-Li, Brüschweiler and Stoodley https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Leggett, Abigail Li, Da-Wei Sindeldecker, Devin Staats, Amelia Rigel, Nicholas Bruschweiler-Li, Lei Brüschweiler, Rafael Stoodley, Paul Cadaverine Is a Switch in the Lysine Degradation Pathway in Pseudomonas aeruginosa Biofilm Identified by Untargeted Metabolomics |
title | Cadaverine Is a Switch in the Lysine Degradation Pathway in Pseudomonas aeruginosa Biofilm Identified by Untargeted Metabolomics |
title_full | Cadaverine Is a Switch in the Lysine Degradation Pathway in Pseudomonas aeruginosa Biofilm Identified by Untargeted Metabolomics |
title_fullStr | Cadaverine Is a Switch in the Lysine Degradation Pathway in Pseudomonas aeruginosa Biofilm Identified by Untargeted Metabolomics |
title_full_unstemmed | Cadaverine Is a Switch in the Lysine Degradation Pathway in Pseudomonas aeruginosa Biofilm Identified by Untargeted Metabolomics |
title_short | Cadaverine Is a Switch in the Lysine Degradation Pathway in Pseudomonas aeruginosa Biofilm Identified by Untargeted Metabolomics |
title_sort | cadaverine is a switch in the lysine degradation pathway in pseudomonas aeruginosa biofilm identified by untargeted metabolomics |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8884266/ https://www.ncbi.nlm.nih.gov/pubmed/35237533 http://dx.doi.org/10.3389/fcimb.2022.833269 |
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