Cargando…

Dysbiosis of Cervical and Vaginal Microbiota Associated With Cervical Intraepithelial Neoplasia

Cervical intraepithelial neoplasia (CIN) is a precancerous condition inducing local lesions on the surface of the squamocolumnar junction of the cervix. Despite the role of vaginal microbiota having been under-discussed, the role of the cervical microbiome and the microbial migration across the repr...

Descripción completa

Detalles Bibliográficos
Autores principales: Lin, Suibin, Zhang, Bin, Lin, Yixia, Lin, Yueping, Zuo, Xiaoyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8885166/
https://www.ncbi.nlm.nih.gov/pubmed/35237529
http://dx.doi.org/10.3389/fcimb.2022.767693
_version_ 1784660342566027264
author Lin, Suibin
Zhang, Bin
Lin, Yixia
Lin, Yueping
Zuo, Xiaoyu
author_facet Lin, Suibin
Zhang, Bin
Lin, Yixia
Lin, Yueping
Zuo, Xiaoyu
author_sort Lin, Suibin
collection PubMed
description Cervical intraepithelial neoplasia (CIN) is a precancerous condition inducing local lesions on the surface of the squamocolumnar junction of the cervix. Despite the role of vaginal microbiota having been under-discussed, the role of the cervical microbiome and the microbial migration across the reproductive tract involved in CIN was limitedly studied. We aimed to synchronously characterize the dysbiosis associated with CIN in both the cervix and vagina in a Chinese population. Profiling of cervical and vaginal microbiota from 60 CIN women and 60 healthy women was conducted. 16S rRNA sequencing was adopted. By comparing the microbial profiles between different parts of the reproductive tract, our results demonstrated an increased shift of microbial diversity in the cervix compared with that in the vagina for the CIN patients, specifically in CIN 1. Less dysbiosis was found between the CIN patients and controls, in either the vagina or cervix. The microbial community may be modulated by the onset of sexual activity, a known clinical risk factor for cervical neoplasia. Distinct patterns of perturbated bacteria were found in the vaginal and cervical microbiota, in which reduced Actinobacteria-related operational taxonomic units (OTUs) and increased Proteobacteria-related OTUs were found in the vagina and cervix, respectively. A good agreement between the direction of the top-significant perturbated OTUs was observed between the vaginal and cervical microbiome, suggesting a potential microbial migration in the reproductive tract. Enriched genera such as Sphingomonas and Stenotrophomonas were found in cervical microbiota-associated CIN. Multivariate analysis revealed Comamonas, Rhizobium, and Pseudomonas as independent genera contributing to CIN in the cervix. In summary, this study revealed the perturbation of microbiota in the presence of CIN and demonstrated a distinct pattern of characteristic bacteria community between the vagina and cervix involved in the development of CIN.
format Online
Article
Text
id pubmed-8885166
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-88851662022-03-01 Dysbiosis of Cervical and Vaginal Microbiota Associated With Cervical Intraepithelial Neoplasia Lin, Suibin Zhang, Bin Lin, Yixia Lin, Yueping Zuo, Xiaoyu Front Cell Infect Microbiol Cellular and Infection Microbiology Cervical intraepithelial neoplasia (CIN) is a precancerous condition inducing local lesions on the surface of the squamocolumnar junction of the cervix. Despite the role of vaginal microbiota having been under-discussed, the role of the cervical microbiome and the microbial migration across the reproductive tract involved in CIN was limitedly studied. We aimed to synchronously characterize the dysbiosis associated with CIN in both the cervix and vagina in a Chinese population. Profiling of cervical and vaginal microbiota from 60 CIN women and 60 healthy women was conducted. 16S rRNA sequencing was adopted. By comparing the microbial profiles between different parts of the reproductive tract, our results demonstrated an increased shift of microbial diversity in the cervix compared with that in the vagina for the CIN patients, specifically in CIN 1. Less dysbiosis was found between the CIN patients and controls, in either the vagina or cervix. The microbial community may be modulated by the onset of sexual activity, a known clinical risk factor for cervical neoplasia. Distinct patterns of perturbated bacteria were found in the vaginal and cervical microbiota, in which reduced Actinobacteria-related operational taxonomic units (OTUs) and increased Proteobacteria-related OTUs were found in the vagina and cervix, respectively. A good agreement between the direction of the top-significant perturbated OTUs was observed between the vaginal and cervical microbiome, suggesting a potential microbial migration in the reproductive tract. Enriched genera such as Sphingomonas and Stenotrophomonas were found in cervical microbiota-associated CIN. Multivariate analysis revealed Comamonas, Rhizobium, and Pseudomonas as independent genera contributing to CIN in the cervix. In summary, this study revealed the perturbation of microbiota in the presence of CIN and demonstrated a distinct pattern of characteristic bacteria community between the vagina and cervix involved in the development of CIN. Frontiers Media S.A. 2022-02-14 /pmc/articles/PMC8885166/ /pubmed/35237529 http://dx.doi.org/10.3389/fcimb.2022.767693 Text en Copyright © 2022 Lin, Zhang, Lin, Lin and Zuo https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Lin, Suibin
Zhang, Bin
Lin, Yixia
Lin, Yueping
Zuo, Xiaoyu
Dysbiosis of Cervical and Vaginal Microbiota Associated With Cervical Intraepithelial Neoplasia
title Dysbiosis of Cervical and Vaginal Microbiota Associated With Cervical Intraepithelial Neoplasia
title_full Dysbiosis of Cervical and Vaginal Microbiota Associated With Cervical Intraepithelial Neoplasia
title_fullStr Dysbiosis of Cervical and Vaginal Microbiota Associated With Cervical Intraepithelial Neoplasia
title_full_unstemmed Dysbiosis of Cervical and Vaginal Microbiota Associated With Cervical Intraepithelial Neoplasia
title_short Dysbiosis of Cervical and Vaginal Microbiota Associated With Cervical Intraepithelial Neoplasia
title_sort dysbiosis of cervical and vaginal microbiota associated with cervical intraepithelial neoplasia
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8885166/
https://www.ncbi.nlm.nih.gov/pubmed/35237529
http://dx.doi.org/10.3389/fcimb.2022.767693
work_keys_str_mv AT linsuibin dysbiosisofcervicalandvaginalmicrobiotaassociatedwithcervicalintraepithelialneoplasia
AT zhangbin dysbiosisofcervicalandvaginalmicrobiotaassociatedwithcervicalintraepithelialneoplasia
AT linyixia dysbiosisofcervicalandvaginalmicrobiotaassociatedwithcervicalintraepithelialneoplasia
AT linyueping dysbiosisofcervicalandvaginalmicrobiotaassociatedwithcervicalintraepithelialneoplasia
AT zuoxiaoyu dysbiosisofcervicalandvaginalmicrobiotaassociatedwithcervicalintraepithelialneoplasia