Cargando…
α-Tocopherol Prevents Sperm Apoptosis and Necrosis in Rats Exposed to 2,3,7,8-Tetrachlorodibenzo-p-dioxin
2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is a persistent organic pollutant that induces overproduction of reactive oxygen species (ROS). Studies on avoiding the adverse effects of dioxin pollution exposure are needed in all aspects, including reproductive health. This study aimed to determine the...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8885270/ https://www.ncbi.nlm.nih.gov/pubmed/35237404 http://dx.doi.org/10.1155/2022/3685686 |
_version_ | 1784660375469293568 |
---|---|
author | Meles, Dewa Ketut Rachmawati, Kadek Hamid, Iwan Sahrial Mustofa, Imam Wurlina, Wurlina Suwasanti, Niluh Putri, Desak Ketut Sekar Cempaka Utama, Suzanita |
author_facet | Meles, Dewa Ketut Rachmawati, Kadek Hamid, Iwan Sahrial Mustofa, Imam Wurlina, Wurlina Suwasanti, Niluh Putri, Desak Ketut Sekar Cempaka Utama, Suzanita |
author_sort | Meles, Dewa Ketut |
collection | PubMed |
description | 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is a persistent organic pollutant that induces overproduction of reactive oxygen species (ROS). Studies on avoiding the adverse effects of dioxin pollution exposure are needed in all aspects, including reproductive health. This study aimed to determine the effect of α-tocopherol on superoxide dismutase (SOD) and malondialdehyde (MDA) levels, live spermatozoa, apoptosis, and necrosis in male rats exposed to dioxin as a model. Thirty healthy 12-week-old male rats were randomly divided into five groups. Rats in the control group were given corn oil twice daily at 4-hour intervals. The remaining rats were given TCDD 700 mg/kg BW daily, followed by administration of corn oil and α-tocopherol at doses of 77, 140, and 259 mg/kg BW/d for T0, T1, T2, and T3 groups, respectively. The treatments were conducted for 45 days; all rats were euthanized to collect blood and testicular samples on day 46. The results showed that exposure of TCDD resulted in a decrease in SOD activity and live spermatozoa and increased MDA level and death, apoptosis, and necrosis of spermatozoa (T0) compared to the control (C) group (p < 0.05). The administration of α-tocopherol, starting from the doses of 77 (T1), 149 (T2), and 259 mg (T3) per kg BW, was sequentially followed by returning MDA levels, recovering SOD activities, and restoration in the percentage of living, dead, apoptotic, and necrotic spermatozoa, similar (p > 0.05) to those of the control group. It could be concluded that the administration of α-tocopherol resolves the harmful effects of TCDD on the viability of spermatozoa in rats as a model. |
format | Online Article Text |
id | pubmed-8885270 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-88852702022-03-01 α-Tocopherol Prevents Sperm Apoptosis and Necrosis in Rats Exposed to 2,3,7,8-Tetrachlorodibenzo-p-dioxin Meles, Dewa Ketut Rachmawati, Kadek Hamid, Iwan Sahrial Mustofa, Imam Wurlina, Wurlina Suwasanti, Niluh Putri, Desak Ketut Sekar Cempaka Utama, Suzanita Vet Med Int Research Article 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is a persistent organic pollutant that induces overproduction of reactive oxygen species (ROS). Studies on avoiding the adverse effects of dioxin pollution exposure are needed in all aspects, including reproductive health. This study aimed to determine the effect of α-tocopherol on superoxide dismutase (SOD) and malondialdehyde (MDA) levels, live spermatozoa, apoptosis, and necrosis in male rats exposed to dioxin as a model. Thirty healthy 12-week-old male rats were randomly divided into five groups. Rats in the control group were given corn oil twice daily at 4-hour intervals. The remaining rats were given TCDD 700 mg/kg BW daily, followed by administration of corn oil and α-tocopherol at doses of 77, 140, and 259 mg/kg BW/d for T0, T1, T2, and T3 groups, respectively. The treatments were conducted for 45 days; all rats were euthanized to collect blood and testicular samples on day 46. The results showed that exposure of TCDD resulted in a decrease in SOD activity and live spermatozoa and increased MDA level and death, apoptosis, and necrosis of spermatozoa (T0) compared to the control (C) group (p < 0.05). The administration of α-tocopherol, starting from the doses of 77 (T1), 149 (T2), and 259 mg (T3) per kg BW, was sequentially followed by returning MDA levels, recovering SOD activities, and restoration in the percentage of living, dead, apoptotic, and necrotic spermatozoa, similar (p > 0.05) to those of the control group. It could be concluded that the administration of α-tocopherol resolves the harmful effects of TCDD on the viability of spermatozoa in rats as a model. Hindawi 2022-02-21 /pmc/articles/PMC8885270/ /pubmed/35237404 http://dx.doi.org/10.1155/2022/3685686 Text en Copyright © 2022 Dewa Ketut Meles et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Meles, Dewa Ketut Rachmawati, Kadek Hamid, Iwan Sahrial Mustofa, Imam Wurlina, Wurlina Suwasanti, Niluh Putri, Desak Ketut Sekar Cempaka Utama, Suzanita α-Tocopherol Prevents Sperm Apoptosis and Necrosis in Rats Exposed to 2,3,7,8-Tetrachlorodibenzo-p-dioxin |
title |
α-Tocopherol Prevents Sperm Apoptosis and Necrosis in Rats Exposed to 2,3,7,8-Tetrachlorodibenzo-p-dioxin |
title_full |
α-Tocopherol Prevents Sperm Apoptosis and Necrosis in Rats Exposed to 2,3,7,8-Tetrachlorodibenzo-p-dioxin |
title_fullStr |
α-Tocopherol Prevents Sperm Apoptosis and Necrosis in Rats Exposed to 2,3,7,8-Tetrachlorodibenzo-p-dioxin |
title_full_unstemmed |
α-Tocopherol Prevents Sperm Apoptosis and Necrosis in Rats Exposed to 2,3,7,8-Tetrachlorodibenzo-p-dioxin |
title_short |
α-Tocopherol Prevents Sperm Apoptosis and Necrosis in Rats Exposed to 2,3,7,8-Tetrachlorodibenzo-p-dioxin |
title_sort | α-tocopherol prevents sperm apoptosis and necrosis in rats exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8885270/ https://www.ncbi.nlm.nih.gov/pubmed/35237404 http://dx.doi.org/10.1155/2022/3685686 |
work_keys_str_mv | AT melesdewaketut atocopherolpreventsspermapoptosisandnecrosisinratsexposedto2378tetrachlorodibenzopdioxin AT rachmawatikadek atocopherolpreventsspermapoptosisandnecrosisinratsexposedto2378tetrachlorodibenzopdioxin AT hamidiwansahrial atocopherolpreventsspermapoptosisandnecrosisinratsexposedto2378tetrachlorodibenzopdioxin AT mustofaimam atocopherolpreventsspermapoptosisandnecrosisinratsexposedto2378tetrachlorodibenzopdioxin AT wurlinawurlina atocopherolpreventsspermapoptosisandnecrosisinratsexposedto2378tetrachlorodibenzopdioxin AT suwasantiniluh atocopherolpreventsspermapoptosisandnecrosisinratsexposedto2378tetrachlorodibenzopdioxin AT putridesakketutsekarcempaka atocopherolpreventsspermapoptosisandnecrosisinratsexposedto2378tetrachlorodibenzopdioxin AT utamasuzanita atocopherolpreventsspermapoptosisandnecrosisinratsexposedto2378tetrachlorodibenzopdioxin |