miR-204-5p is sponged by TUG1 to aggravate neuron damage induced by focal cerebral ischemia and reperfusion injury through upregulating COX2

Studies have reported that miR-204-5p is involved in multiple biological processes. However, little is known about the expression and mechanism of miR-204-5p in cerebral ischemia and reperfusion injury. This study found that miR-204-5p expression was significantly downregulated in the blood of patie...

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Autores principales: Xiang, Pu, Hu, Jian, Wang, Hong, Luo, Ying, Gu, Chao, Tan, Xiaodan, Tu, Yujun, Guo, Wenjia, Chen, Lin, Gao, Lin, Chen, Rongchun, Yang, Junqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8885635/
https://www.ncbi.nlm.nih.gov/pubmed/35228515
http://dx.doi.org/10.1038/s41420-022-00885-x
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author Xiang, Pu
Hu, Jian
Wang, Hong
Luo, Ying
Gu, Chao
Tan, Xiaodan
Tu, Yujun
Guo, Wenjia
Chen, Lin
Gao, Lin
Chen, Rongchun
Yang, Junqing
author_facet Xiang, Pu
Hu, Jian
Wang, Hong
Luo, Ying
Gu, Chao
Tan, Xiaodan
Tu, Yujun
Guo, Wenjia
Chen, Lin
Gao, Lin
Chen, Rongchun
Yang, Junqing
author_sort Xiang, Pu
collection PubMed
description Studies have reported that miR-204-5p is involved in multiple biological processes. However, little is known about the expression and mechanism of miR-204-5p in cerebral ischemia and reperfusion injury. This study found that miR-204-5p expression was significantly downregulated in the blood of patients with ischemic stroke, MCAO/R rat brains, and OGD/R neurons. Overexpression of miR-204-5p markedly reduced infarct volume and neurological impairment and alleviated the inflammatory response in vivo. miR-204-5p promoted neuronal viability and reduced apoptotic cells in vitro. Mechanically, miR-204-5p was negatively regulated by the expression lncRNA TUG1 upstream and down-regulated COX2 expression downstream. Therefore, the TUG1/miR-204-5p/COX2 axis was involved in ischemia and reperfusion-induced neuronal damage. This finding may provide a novel strategy for the treatment of cerebral ischemia and reperfusion injury.
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spelling pubmed-88856352022-03-17 miR-204-5p is sponged by TUG1 to aggravate neuron damage induced by focal cerebral ischemia and reperfusion injury through upregulating COX2 Xiang, Pu Hu, Jian Wang, Hong Luo, Ying Gu, Chao Tan, Xiaodan Tu, Yujun Guo, Wenjia Chen, Lin Gao, Lin Chen, Rongchun Yang, Junqing Cell Death Discov Article Studies have reported that miR-204-5p is involved in multiple biological processes. However, little is known about the expression and mechanism of miR-204-5p in cerebral ischemia and reperfusion injury. This study found that miR-204-5p expression was significantly downregulated in the blood of patients with ischemic stroke, MCAO/R rat brains, and OGD/R neurons. Overexpression of miR-204-5p markedly reduced infarct volume and neurological impairment and alleviated the inflammatory response in vivo. miR-204-5p promoted neuronal viability and reduced apoptotic cells in vitro. Mechanically, miR-204-5p was negatively regulated by the expression lncRNA TUG1 upstream and down-regulated COX2 expression downstream. Therefore, the TUG1/miR-204-5p/COX2 axis was involved in ischemia and reperfusion-induced neuronal damage. This finding may provide a novel strategy for the treatment of cerebral ischemia and reperfusion injury. Nature Publishing Group UK 2022-02-28 /pmc/articles/PMC8885635/ /pubmed/35228515 http://dx.doi.org/10.1038/s41420-022-00885-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Xiang, Pu
Hu, Jian
Wang, Hong
Luo, Ying
Gu, Chao
Tan, Xiaodan
Tu, Yujun
Guo, Wenjia
Chen, Lin
Gao, Lin
Chen, Rongchun
Yang, Junqing
miR-204-5p is sponged by TUG1 to aggravate neuron damage induced by focal cerebral ischemia and reperfusion injury through upregulating COX2
title miR-204-5p is sponged by TUG1 to aggravate neuron damage induced by focal cerebral ischemia and reperfusion injury through upregulating COX2
title_full miR-204-5p is sponged by TUG1 to aggravate neuron damage induced by focal cerebral ischemia and reperfusion injury through upregulating COX2
title_fullStr miR-204-5p is sponged by TUG1 to aggravate neuron damage induced by focal cerebral ischemia and reperfusion injury through upregulating COX2
title_full_unstemmed miR-204-5p is sponged by TUG1 to aggravate neuron damage induced by focal cerebral ischemia and reperfusion injury through upregulating COX2
title_short miR-204-5p is sponged by TUG1 to aggravate neuron damage induced by focal cerebral ischemia and reperfusion injury through upregulating COX2
title_sort mir-204-5p is sponged by tug1 to aggravate neuron damage induced by focal cerebral ischemia and reperfusion injury through upregulating cox2
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8885635/
https://www.ncbi.nlm.nih.gov/pubmed/35228515
http://dx.doi.org/10.1038/s41420-022-00885-x
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