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Kastor and Polluks polypeptides encoded by a single gene locus cooperatively regulate VDAC and spermatogenesis
Although several long noncoding RNAs (lncRNAs) have recently been shown to encode small polypeptides, those in testis remain largely uncharacterized. Here we identify two sperm-specific polypeptides, Kastor and Polluks, encoded by a single mouse locus (Gm9999) previously annotated as encoding a lncR...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8885739/ https://www.ncbi.nlm.nih.gov/pubmed/35228556 http://dx.doi.org/10.1038/s41467-022-28677-y |
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author | Mise, Shintaro Matsumoto, Akinobu Shimada, Keisuke Hosaka, Toshiaki Takahashi, Masatomo Ichihara, Kazuya Shimizu, Hideyuki Shiraishi, Chisa Saito, Daisuke Suyama, Mikita Yasuda, Tomoharu Ide, Toru Izumi, Yoshihiro Bamba, Takeshi Kimura-Someya, Tomomi Shirouzu, Mikako Miyata, Haruhiko Ikawa, Masahito Nakayama, Keiichi I. |
author_facet | Mise, Shintaro Matsumoto, Akinobu Shimada, Keisuke Hosaka, Toshiaki Takahashi, Masatomo Ichihara, Kazuya Shimizu, Hideyuki Shiraishi, Chisa Saito, Daisuke Suyama, Mikita Yasuda, Tomoharu Ide, Toru Izumi, Yoshihiro Bamba, Takeshi Kimura-Someya, Tomomi Shirouzu, Mikako Miyata, Haruhiko Ikawa, Masahito Nakayama, Keiichi I. |
author_sort | Mise, Shintaro |
collection | PubMed |
description | Although several long noncoding RNAs (lncRNAs) have recently been shown to encode small polypeptides, those in testis remain largely uncharacterized. Here we identify two sperm-specific polypeptides, Kastor and Polluks, encoded by a single mouse locus (Gm9999) previously annotated as encoding a lncRNA. Both Kastor and Polluks are inserted in the outer mitochondrial membrane and directly interact with voltage-dependent anion channel (VDAC), despite their different amino acid sequences. Male VDAC3-deficient mice are infertile as a result of reduced sperm motility due to an abnormal mitochondrial sheath in spermatozoa, and deficiency of both Kastor and Polluks also severely impaired male fertility in association with formation of a similarly abnormal mitochondrial sheath. Spermatozoa lacking either Kastor or Polluks partially recapitulate the phenotype of those lacking both. Cooperative function of Kastor and Polluks in regulation of VDAC3 may thus be essential for mitochondrial sheath formation in spermatozoa and for male fertility. |
format | Online Article Text |
id | pubmed-8885739 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-88857392022-03-17 Kastor and Polluks polypeptides encoded by a single gene locus cooperatively regulate VDAC and spermatogenesis Mise, Shintaro Matsumoto, Akinobu Shimada, Keisuke Hosaka, Toshiaki Takahashi, Masatomo Ichihara, Kazuya Shimizu, Hideyuki Shiraishi, Chisa Saito, Daisuke Suyama, Mikita Yasuda, Tomoharu Ide, Toru Izumi, Yoshihiro Bamba, Takeshi Kimura-Someya, Tomomi Shirouzu, Mikako Miyata, Haruhiko Ikawa, Masahito Nakayama, Keiichi I. Nat Commun Article Although several long noncoding RNAs (lncRNAs) have recently been shown to encode small polypeptides, those in testis remain largely uncharacterized. Here we identify two sperm-specific polypeptides, Kastor and Polluks, encoded by a single mouse locus (Gm9999) previously annotated as encoding a lncRNA. Both Kastor and Polluks are inserted in the outer mitochondrial membrane and directly interact with voltage-dependent anion channel (VDAC), despite their different amino acid sequences. Male VDAC3-deficient mice are infertile as a result of reduced sperm motility due to an abnormal mitochondrial sheath in spermatozoa, and deficiency of both Kastor and Polluks also severely impaired male fertility in association with formation of a similarly abnormal mitochondrial sheath. Spermatozoa lacking either Kastor or Polluks partially recapitulate the phenotype of those lacking both. Cooperative function of Kastor and Polluks in regulation of VDAC3 may thus be essential for mitochondrial sheath formation in spermatozoa and for male fertility. Nature Publishing Group UK 2022-02-28 /pmc/articles/PMC8885739/ /pubmed/35228556 http://dx.doi.org/10.1038/s41467-022-28677-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Mise, Shintaro Matsumoto, Akinobu Shimada, Keisuke Hosaka, Toshiaki Takahashi, Masatomo Ichihara, Kazuya Shimizu, Hideyuki Shiraishi, Chisa Saito, Daisuke Suyama, Mikita Yasuda, Tomoharu Ide, Toru Izumi, Yoshihiro Bamba, Takeshi Kimura-Someya, Tomomi Shirouzu, Mikako Miyata, Haruhiko Ikawa, Masahito Nakayama, Keiichi I. Kastor and Polluks polypeptides encoded by a single gene locus cooperatively regulate VDAC and spermatogenesis |
title | Kastor and Polluks polypeptides encoded by a single gene locus cooperatively regulate VDAC and spermatogenesis |
title_full | Kastor and Polluks polypeptides encoded by a single gene locus cooperatively regulate VDAC and spermatogenesis |
title_fullStr | Kastor and Polluks polypeptides encoded by a single gene locus cooperatively regulate VDAC and spermatogenesis |
title_full_unstemmed | Kastor and Polluks polypeptides encoded by a single gene locus cooperatively regulate VDAC and spermatogenesis |
title_short | Kastor and Polluks polypeptides encoded by a single gene locus cooperatively regulate VDAC and spermatogenesis |
title_sort | kastor and polluks polypeptides encoded by a single gene locus cooperatively regulate vdac and spermatogenesis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8885739/ https://www.ncbi.nlm.nih.gov/pubmed/35228556 http://dx.doi.org/10.1038/s41467-022-28677-y |
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