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Microfluidic channel sensory system for electro-addressing cell location, determining confluency, and quantifying a general number of cells

Microfluidics is a highly useful platform for culturing, monitoring, and testing biological cells. The integration of electrodes into microfluidic channels extends the functionality, sensing, and testing capabilities of microfluidic systems. By employing an electrochemical impedance spectroscopy (EI...

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Autores principales: Rapier, Crystal E., Jagadeesan, Srikanth, Vatine, Gad, Ben-Yoav, Hadar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8885753/
https://www.ncbi.nlm.nih.gov/pubmed/35228609
http://dx.doi.org/10.1038/s41598-022-07194-4
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author Rapier, Crystal E.
Jagadeesan, Srikanth
Vatine, Gad
Ben-Yoav, Hadar
author_facet Rapier, Crystal E.
Jagadeesan, Srikanth
Vatine, Gad
Ben-Yoav, Hadar
author_sort Rapier, Crystal E.
collection PubMed
description Microfluidics is a highly useful platform for culturing, monitoring, and testing biological cells. The integration of electrodes into microfluidic channels extends the functionality, sensing, and testing capabilities of microfluidic systems. By employing an electrochemical impedance spectroscopy (EIS) technique, the non-invasive, label-free detection of the activities of cells in real-time can be achieved. To address the movement toward spatially resolving cells in cell culture, we developed a sensory system capable of electro-addressing cell location within a microfluidic channel. This simple system allows for real-time cell location, integrity monitoring (of barrier producing cells), and confluency sensing without the need for frequent optical evaluation—saving time. EIS results demonstrate that cells within microfluidic channels can be located between various pairs of electrodes at different positions along the length of the device. Impedance spectra clearly differentiates between empty, sparse, and confluent microfluidic channels. The system also senses the level of cell confluence between electrode pairs—allowing for the relative quantification of cells in different areas of the microfluidic channel. The system’s electrode layout can easily be incorporated into other devices. Namely, organ-on-a-chip devices, that require the monitoring of precise cell location and confluency levels for understanding tissue function, modeling diseases, and for testing therapeutics.
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spelling pubmed-88857532022-03-01 Microfluidic channel sensory system for electro-addressing cell location, determining confluency, and quantifying a general number of cells Rapier, Crystal E. Jagadeesan, Srikanth Vatine, Gad Ben-Yoav, Hadar Sci Rep Article Microfluidics is a highly useful platform for culturing, monitoring, and testing biological cells. The integration of electrodes into microfluidic channels extends the functionality, sensing, and testing capabilities of microfluidic systems. By employing an electrochemical impedance spectroscopy (EIS) technique, the non-invasive, label-free detection of the activities of cells in real-time can be achieved. To address the movement toward spatially resolving cells in cell culture, we developed a sensory system capable of electro-addressing cell location within a microfluidic channel. This simple system allows for real-time cell location, integrity monitoring (of barrier producing cells), and confluency sensing without the need for frequent optical evaluation—saving time. EIS results demonstrate that cells within microfluidic channels can be located between various pairs of electrodes at different positions along the length of the device. Impedance spectra clearly differentiates between empty, sparse, and confluent microfluidic channels. The system also senses the level of cell confluence between electrode pairs—allowing for the relative quantification of cells in different areas of the microfluidic channel. The system’s electrode layout can easily be incorporated into other devices. Namely, organ-on-a-chip devices, that require the monitoring of precise cell location and confluency levels for understanding tissue function, modeling diseases, and for testing therapeutics. Nature Publishing Group UK 2022-02-28 /pmc/articles/PMC8885753/ /pubmed/35228609 http://dx.doi.org/10.1038/s41598-022-07194-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Rapier, Crystal E.
Jagadeesan, Srikanth
Vatine, Gad
Ben-Yoav, Hadar
Microfluidic channel sensory system for electro-addressing cell location, determining confluency, and quantifying a general number of cells
title Microfluidic channel sensory system for electro-addressing cell location, determining confluency, and quantifying a general number of cells
title_full Microfluidic channel sensory system for electro-addressing cell location, determining confluency, and quantifying a general number of cells
title_fullStr Microfluidic channel sensory system for electro-addressing cell location, determining confluency, and quantifying a general number of cells
title_full_unstemmed Microfluidic channel sensory system for electro-addressing cell location, determining confluency, and quantifying a general number of cells
title_short Microfluidic channel sensory system for electro-addressing cell location, determining confluency, and quantifying a general number of cells
title_sort microfluidic channel sensory system for electro-addressing cell location, determining confluency, and quantifying a general number of cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8885753/
https://www.ncbi.nlm.nih.gov/pubmed/35228609
http://dx.doi.org/10.1038/s41598-022-07194-4
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