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Optimization of supercritical-CO(2) extraction and pharmacokinetics in SD rats of alkaloids form Sophora moorcroftiana seed

The total alkaloids extracted from the seeds of Sophora moorcroftiana (TAs-SM) have the potential to treat alveolar echinococcosis, a disease included by the WHO in a list of 17 key neglected diseases world-wide. The aims of the current study were first to develop a supercritical fluid extraction (S...

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Autores principales: Hu, Chunhui, Gan, Xuehui, Jia, Qiangqiang, Gao, Pan, Du, Tao, Zhang, Fabin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8885761/
https://www.ncbi.nlm.nih.gov/pubmed/35228632
http://dx.doi.org/10.1038/s41598-022-07278-1
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author Hu, Chunhui
Gan, Xuehui
Jia, Qiangqiang
Gao, Pan
Du, Tao
Zhang, Fabin
author_facet Hu, Chunhui
Gan, Xuehui
Jia, Qiangqiang
Gao, Pan
Du, Tao
Zhang, Fabin
author_sort Hu, Chunhui
collection PubMed
description The total alkaloids extracted from the seeds of Sophora moorcroftiana (TAs-SM) have the potential to treat alveolar echinococcosis, a disease included by the WHO in a list of 17 key neglected diseases world-wide. The aims of the current study were first to develop a supercritical fluid extraction (SFE) method for optimizing TAs-SM extraction, and second, to develop an optimized method for evaluating TAs-SM pharmacokinetics in vivo. The Box–Behnken response surface method was used to optimize the extraction process, and ultra-high liquid chromatography coupled with high resolution electrospray mass spectrometry (UPLC-HR-ESI-MS) was used to determine the pharmacokinetics of TAs-SM in SD rats. The results indicated the following optimal SFE extraction conditions: pressure = 31 MPa, temperature = 70 °C, time = 162.18 min. With these parameters, total alkaloids could be extracted from each gram of S. moorcroftiana, with the total content being 68.88 μg. The linear range of UPLC-HR-ESI-MS is 0.78–200.00 ng/ml, R(2) > 0.99, and the sample recovery is 99–113%. The precision, accuracy, selectivity and stability of the method meet the requirements of US FDA guidelines. To our knowledge this study is the first to establish an SFE method for extracting TAs-SM and the first to employ UPLC-HR-ESI-MS for measuring TAs-SM in rats. These findings provide important contributions for using TAs-SM in further drug development and clinical applications.
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spelling pubmed-88857612022-03-01 Optimization of supercritical-CO(2) extraction and pharmacokinetics in SD rats of alkaloids form Sophora moorcroftiana seed Hu, Chunhui Gan, Xuehui Jia, Qiangqiang Gao, Pan Du, Tao Zhang, Fabin Sci Rep Article The total alkaloids extracted from the seeds of Sophora moorcroftiana (TAs-SM) have the potential to treat alveolar echinococcosis, a disease included by the WHO in a list of 17 key neglected diseases world-wide. The aims of the current study were first to develop a supercritical fluid extraction (SFE) method for optimizing TAs-SM extraction, and second, to develop an optimized method for evaluating TAs-SM pharmacokinetics in vivo. The Box–Behnken response surface method was used to optimize the extraction process, and ultra-high liquid chromatography coupled with high resolution electrospray mass spectrometry (UPLC-HR-ESI-MS) was used to determine the pharmacokinetics of TAs-SM in SD rats. The results indicated the following optimal SFE extraction conditions: pressure = 31 MPa, temperature = 70 °C, time = 162.18 min. With these parameters, total alkaloids could be extracted from each gram of S. moorcroftiana, with the total content being 68.88 μg. The linear range of UPLC-HR-ESI-MS is 0.78–200.00 ng/ml, R(2) > 0.99, and the sample recovery is 99–113%. The precision, accuracy, selectivity and stability of the method meet the requirements of US FDA guidelines. To our knowledge this study is the first to establish an SFE method for extracting TAs-SM and the first to employ UPLC-HR-ESI-MS for measuring TAs-SM in rats. These findings provide important contributions for using TAs-SM in further drug development and clinical applications. Nature Publishing Group UK 2022-02-28 /pmc/articles/PMC8885761/ /pubmed/35228632 http://dx.doi.org/10.1038/s41598-022-07278-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Hu, Chunhui
Gan, Xuehui
Jia, Qiangqiang
Gao, Pan
Du, Tao
Zhang, Fabin
Optimization of supercritical-CO(2) extraction and pharmacokinetics in SD rats of alkaloids form Sophora moorcroftiana seed
title Optimization of supercritical-CO(2) extraction and pharmacokinetics in SD rats of alkaloids form Sophora moorcroftiana seed
title_full Optimization of supercritical-CO(2) extraction and pharmacokinetics in SD rats of alkaloids form Sophora moorcroftiana seed
title_fullStr Optimization of supercritical-CO(2) extraction and pharmacokinetics in SD rats of alkaloids form Sophora moorcroftiana seed
title_full_unstemmed Optimization of supercritical-CO(2) extraction and pharmacokinetics in SD rats of alkaloids form Sophora moorcroftiana seed
title_short Optimization of supercritical-CO(2) extraction and pharmacokinetics in SD rats of alkaloids form Sophora moorcroftiana seed
title_sort optimization of supercritical-co(2) extraction and pharmacokinetics in sd rats of alkaloids form sophora moorcroftiana seed
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8885761/
https://www.ncbi.nlm.nih.gov/pubmed/35228632
http://dx.doi.org/10.1038/s41598-022-07278-1
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