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Protocol to isolate cells in four stages of S phase for high-resolution replication-timing sequencing
Traditional replication timing (RT) experiments divide S phase into two phases: early and late. However, there is an increasing awareness that variation in RT can occur during the course of S phase and impact our understanding of RT patterns and regulation. Here, we describe a RT protocol in RPE-1 c...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8885763/ https://www.ncbi.nlm.nih.gov/pubmed/35243385 http://dx.doi.org/10.1016/j.xpro.2022.101209 |