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Crystallographic characterization of a marine invertebrate ferritin from the sea cucumber Apostichopus japonicus

Ferritin is considered to be an ubiquitous and conserved iron‐binding protein that plays a crucial role in iron storage, detoxification, and immune response. Although ferritin is of critical importance for almost all kingdoms of life, there is a lack of knowledge about its role in the marine inverte...

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Autores principales: Wu, Yan, Ming, Tinghong, Huo, Chunheng, Qiu, Xiaoting, Su, Chang, Lu, Chenyang, Zhou, Jun, Li, Ye, Su, Xiurong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8886333/
https://www.ncbi.nlm.nih.gov/pubmed/35090095
http://dx.doi.org/10.1002/2211-5463.13375
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author Wu, Yan
Ming, Tinghong
Huo, Chunheng
Qiu, Xiaoting
Su, Chang
Lu, Chenyang
Zhou, Jun
Li, Ye
Su, Xiurong
author_facet Wu, Yan
Ming, Tinghong
Huo, Chunheng
Qiu, Xiaoting
Su, Chang
Lu, Chenyang
Zhou, Jun
Li, Ye
Su, Xiurong
author_sort Wu, Yan
collection PubMed
description Ferritin is considered to be an ubiquitous and conserved iron‐binding protein that plays a crucial role in iron storage, detoxification, and immune response. Although ferritin is of critical importance for almost all kingdoms of life, there is a lack of knowledge about its role in the marine invertebrate sea cucumber (Apostichopus japonicus). In this study, we characterized the first crystal structure of A. japonicus ferritin (AjFER) at 2.75 Å resolution. The structure of AjFER shows a 4‐3‐2 symmetry cage‐like hollow shell composed of 24 subunits, mostly similar to the structural characteristics of other known ferritin species, including the conserved ferroxidase center and 3‐fold channel. The 3‐fold channel consisting of three 3‐fold negative amino acid rings suggests a potential pathway in which metal ions can be first captured by Asp120 from the outside environment, attracted by His116 and Cys128 when entering the channel, and then transferred by Glu138 from the 3‐fold channel to the ferroxidase site. Overall, the presented crystal structure of AjFER may provide insights into the potential mechanism of the metal transport pathway for related marine invertebrate ferritins.
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spelling pubmed-88863332022-03-04 Crystallographic characterization of a marine invertebrate ferritin from the sea cucumber Apostichopus japonicus Wu, Yan Ming, Tinghong Huo, Chunheng Qiu, Xiaoting Su, Chang Lu, Chenyang Zhou, Jun Li, Ye Su, Xiurong FEBS Open Bio Research Articles Ferritin is considered to be an ubiquitous and conserved iron‐binding protein that plays a crucial role in iron storage, detoxification, and immune response. Although ferritin is of critical importance for almost all kingdoms of life, there is a lack of knowledge about its role in the marine invertebrate sea cucumber (Apostichopus japonicus). In this study, we characterized the first crystal structure of A. japonicus ferritin (AjFER) at 2.75 Å resolution. The structure of AjFER shows a 4‐3‐2 symmetry cage‐like hollow shell composed of 24 subunits, mostly similar to the structural characteristics of other known ferritin species, including the conserved ferroxidase center and 3‐fold channel. The 3‐fold channel consisting of three 3‐fold negative amino acid rings suggests a potential pathway in which metal ions can be first captured by Asp120 from the outside environment, attracted by His116 and Cys128 when entering the channel, and then transferred by Glu138 from the 3‐fold channel to the ferroxidase site. Overall, the presented crystal structure of AjFER may provide insights into the potential mechanism of the metal transport pathway for related marine invertebrate ferritins. John Wiley and Sons Inc. 2022-02-07 /pmc/articles/PMC8886333/ /pubmed/35090095 http://dx.doi.org/10.1002/2211-5463.13375 Text en © 2022 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Wu, Yan
Ming, Tinghong
Huo, Chunheng
Qiu, Xiaoting
Su, Chang
Lu, Chenyang
Zhou, Jun
Li, Ye
Su, Xiurong
Crystallographic characterization of a marine invertebrate ferritin from the sea cucumber Apostichopus japonicus
title Crystallographic characterization of a marine invertebrate ferritin from the sea cucumber Apostichopus japonicus
title_full Crystallographic characterization of a marine invertebrate ferritin from the sea cucumber Apostichopus japonicus
title_fullStr Crystallographic characterization of a marine invertebrate ferritin from the sea cucumber Apostichopus japonicus
title_full_unstemmed Crystallographic characterization of a marine invertebrate ferritin from the sea cucumber Apostichopus japonicus
title_short Crystallographic characterization of a marine invertebrate ferritin from the sea cucumber Apostichopus japonicus
title_sort crystallographic characterization of a marine invertebrate ferritin from the sea cucumber apostichopus japonicus
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8886333/
https://www.ncbi.nlm.nih.gov/pubmed/35090095
http://dx.doi.org/10.1002/2211-5463.13375
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