Cargando…

Hdac4 Regulates the Proliferation of Neural Crest-Derived Osteoblasts During Murine Craniofacial Development

Craniofacial development involves the regulation of a compendium of transcription factors, signaling molecules, and epigenetic regulators. Histone deacetylases (HDACs) are involved in the regulation of cell proliferation, differentiation, and homeostasis across a wide range of tissues, including the...

Descripción completa

Detalles Bibliográficos
Autores principales: Ha, Nayoung, Sun, Jian, Bian, Qian, Wu, Dandan, Wang, Xudong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8886889/
https://www.ncbi.nlm.nih.gov/pubmed/35242053
http://dx.doi.org/10.3389/fphys.2022.819619
_version_ 1784660778449633280
author Ha, Nayoung
Sun, Jian
Bian, Qian
Wu, Dandan
Wang, Xudong
author_facet Ha, Nayoung
Sun, Jian
Bian, Qian
Wu, Dandan
Wang, Xudong
author_sort Ha, Nayoung
collection PubMed
description Craniofacial development involves the regulation of a compendium of transcription factors, signaling molecules, and epigenetic regulators. Histone deacetylases (HDACs) are involved in the regulation of cell proliferation, differentiation, and homeostasis across a wide range of tissues, including the brain and the cardiovascular, muscular, and skeletal systems. However, the functional role of Hdac4 during craniofacial development remains unclear. In this study, we investigated the effects of knocking out Hdac4 on craniofacial skeletal development by conditionally disrupting the Hdac4 gene in cranial neural crest cells (CNCCs) using Cre-mediated recombination. Mice deficient for Hdac4 in CNCC-derived osteoblasts demonstrated a dramatic decrease in frontal bone formation. In vitro, pre-osteoblasts (MC3T3-E1 cells) lacking Hdac4 exhibited reduced proliferative activity in association with the dysregulation of cell cycle-related genes. These findings suggested that Hdac4 acts, at least in part, as a regulator of craniofacial skeletal development by positively regulating the proliferation of CNCC-derived osteoblasts.
format Online
Article
Text
id pubmed-8886889
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-88868892022-03-02 Hdac4 Regulates the Proliferation of Neural Crest-Derived Osteoblasts During Murine Craniofacial Development Ha, Nayoung Sun, Jian Bian, Qian Wu, Dandan Wang, Xudong Front Physiol Physiology Craniofacial development involves the regulation of a compendium of transcription factors, signaling molecules, and epigenetic regulators. Histone deacetylases (HDACs) are involved in the regulation of cell proliferation, differentiation, and homeostasis across a wide range of tissues, including the brain and the cardiovascular, muscular, and skeletal systems. However, the functional role of Hdac4 during craniofacial development remains unclear. In this study, we investigated the effects of knocking out Hdac4 on craniofacial skeletal development by conditionally disrupting the Hdac4 gene in cranial neural crest cells (CNCCs) using Cre-mediated recombination. Mice deficient for Hdac4 in CNCC-derived osteoblasts demonstrated a dramatic decrease in frontal bone formation. In vitro, pre-osteoblasts (MC3T3-E1 cells) lacking Hdac4 exhibited reduced proliferative activity in association with the dysregulation of cell cycle-related genes. These findings suggested that Hdac4 acts, at least in part, as a regulator of craniofacial skeletal development by positively regulating the proliferation of CNCC-derived osteoblasts. Frontiers Media S.A. 2022-02-15 /pmc/articles/PMC8886889/ /pubmed/35242053 http://dx.doi.org/10.3389/fphys.2022.819619 Text en Copyright © 2022 Ha, Sun, Bian, Wu and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Ha, Nayoung
Sun, Jian
Bian, Qian
Wu, Dandan
Wang, Xudong
Hdac4 Regulates the Proliferation of Neural Crest-Derived Osteoblasts During Murine Craniofacial Development
title Hdac4 Regulates the Proliferation of Neural Crest-Derived Osteoblasts During Murine Craniofacial Development
title_full Hdac4 Regulates the Proliferation of Neural Crest-Derived Osteoblasts During Murine Craniofacial Development
title_fullStr Hdac4 Regulates the Proliferation of Neural Crest-Derived Osteoblasts During Murine Craniofacial Development
title_full_unstemmed Hdac4 Regulates the Proliferation of Neural Crest-Derived Osteoblasts During Murine Craniofacial Development
title_short Hdac4 Regulates the Proliferation of Neural Crest-Derived Osteoblasts During Murine Craniofacial Development
title_sort hdac4 regulates the proliferation of neural crest-derived osteoblasts during murine craniofacial development
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8886889/
https://www.ncbi.nlm.nih.gov/pubmed/35242053
http://dx.doi.org/10.3389/fphys.2022.819619
work_keys_str_mv AT hanayoung hdac4regulatestheproliferationofneuralcrestderivedosteoblastsduringmurinecraniofacialdevelopment
AT sunjian hdac4regulatestheproliferationofneuralcrestderivedosteoblastsduringmurinecraniofacialdevelopment
AT bianqian hdac4regulatestheproliferationofneuralcrestderivedosteoblastsduringmurinecraniofacialdevelopment
AT wudandan hdac4regulatestheproliferationofneuralcrestderivedosteoblastsduringmurinecraniofacialdevelopment
AT wangxudong hdac4regulatestheproliferationofneuralcrestderivedosteoblastsduringmurinecraniofacialdevelopment