Cargando…
High throughput single-cell genome sequencing gives insights into the generation and evolution of mosaic aneuploidy in Leishmania donovani
Leishmania, a unicellular eukaryotic parasite, is a unique model for aneuploidy and cellular heterogeneity, along with their potential role in adaptation to environmental stresses. Somy variation within clonal populations was previously explored in a small subset of chromosomes using fluorescence hy...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8886908/ https://www.ncbi.nlm.nih.gov/pubmed/34893872 http://dx.doi.org/10.1093/nar/gkab1203 |
_version_ | 1784660781446463488 |
---|---|
author | Negreira, Gabriel H Monsieurs, Pieter Imamura, Hideo Maes, Ilse Kuk, Nada Yagoubat, Akila Van den Broeck, Frederik Sterkers, Yvon Dujardin, Jean-Claude Domagalska, Malgorzata A |
author_facet | Negreira, Gabriel H Monsieurs, Pieter Imamura, Hideo Maes, Ilse Kuk, Nada Yagoubat, Akila Van den Broeck, Frederik Sterkers, Yvon Dujardin, Jean-Claude Domagalska, Malgorzata A |
author_sort | Negreira, Gabriel H |
collection | PubMed |
description | Leishmania, a unicellular eukaryotic parasite, is a unique model for aneuploidy and cellular heterogeneity, along with their potential role in adaptation to environmental stresses. Somy variation within clonal populations was previously explored in a small subset of chromosomes using fluorescence hybridization methods. This phenomenon, termed mosaic aneuploidy (MA), might have important evolutionary and functional implications but remains under-explored due to technological limitations. Here, we applied and validated a high throughput single-cell genome sequencing method to study for the first time the extent and dynamics of whole karyotype heterogeneity in two clonal populations of Leishmania promastigotes representing different stages of MA evolution in vitro. We found that drastic changes in karyotypes quickly emerge in a population stemming from an almost euploid founder cell. This possibly involves polyploidization/hybridization at an early stage of population expansion, followed by assorted ploidy reduction. During further stages of expansion, MA increases by moderate and gradual karyotypic alterations, affecting a defined subset of chromosomes. Our data provide the first complete characterization of MA in Leishmania and pave the way for further functional studies. |
format | Online Article Text |
id | pubmed-8886908 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-88869082022-03-02 High throughput single-cell genome sequencing gives insights into the generation and evolution of mosaic aneuploidy in Leishmania donovani Negreira, Gabriel H Monsieurs, Pieter Imamura, Hideo Maes, Ilse Kuk, Nada Yagoubat, Akila Van den Broeck, Frederik Sterkers, Yvon Dujardin, Jean-Claude Domagalska, Malgorzata A Nucleic Acids Res Genomics Leishmania, a unicellular eukaryotic parasite, is a unique model for aneuploidy and cellular heterogeneity, along with their potential role in adaptation to environmental stresses. Somy variation within clonal populations was previously explored in a small subset of chromosomes using fluorescence hybridization methods. This phenomenon, termed mosaic aneuploidy (MA), might have important evolutionary and functional implications but remains under-explored due to technological limitations. Here, we applied and validated a high throughput single-cell genome sequencing method to study for the first time the extent and dynamics of whole karyotype heterogeneity in two clonal populations of Leishmania promastigotes representing different stages of MA evolution in vitro. We found that drastic changes in karyotypes quickly emerge in a population stemming from an almost euploid founder cell. This possibly involves polyploidization/hybridization at an early stage of population expansion, followed by assorted ploidy reduction. During further stages of expansion, MA increases by moderate and gradual karyotypic alterations, affecting a defined subset of chromosomes. Our data provide the first complete characterization of MA in Leishmania and pave the way for further functional studies. Oxford University Press 2021-12-10 /pmc/articles/PMC8886908/ /pubmed/34893872 http://dx.doi.org/10.1093/nar/gkab1203 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Genomics Negreira, Gabriel H Monsieurs, Pieter Imamura, Hideo Maes, Ilse Kuk, Nada Yagoubat, Akila Van den Broeck, Frederik Sterkers, Yvon Dujardin, Jean-Claude Domagalska, Malgorzata A High throughput single-cell genome sequencing gives insights into the generation and evolution of mosaic aneuploidy in Leishmania donovani |
title | High throughput single-cell genome sequencing gives insights into the
generation and evolution of mosaic aneuploidy in Leishmania
donovani |
title_full | High throughput single-cell genome sequencing gives insights into the
generation and evolution of mosaic aneuploidy in Leishmania
donovani |
title_fullStr | High throughput single-cell genome sequencing gives insights into the
generation and evolution of mosaic aneuploidy in Leishmania
donovani |
title_full_unstemmed | High throughput single-cell genome sequencing gives insights into the
generation and evolution of mosaic aneuploidy in Leishmania
donovani |
title_short | High throughput single-cell genome sequencing gives insights into the
generation and evolution of mosaic aneuploidy in Leishmania
donovani |
title_sort | high throughput single-cell genome sequencing gives insights into the
generation and evolution of mosaic aneuploidy in leishmania
donovani |
topic | Genomics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8886908/ https://www.ncbi.nlm.nih.gov/pubmed/34893872 http://dx.doi.org/10.1093/nar/gkab1203 |
work_keys_str_mv | AT negreiragabrielh highthroughputsinglecellgenomesequencinggivesinsightsintothegenerationandevolutionofmosaicaneuploidyinleishmaniadonovani AT monsieurspieter highthroughputsinglecellgenomesequencinggivesinsightsintothegenerationandevolutionofmosaicaneuploidyinleishmaniadonovani AT imamurahideo highthroughputsinglecellgenomesequencinggivesinsightsintothegenerationandevolutionofmosaicaneuploidyinleishmaniadonovani AT maesilse highthroughputsinglecellgenomesequencinggivesinsightsintothegenerationandevolutionofmosaicaneuploidyinleishmaniadonovani AT kuknada highthroughputsinglecellgenomesequencinggivesinsightsintothegenerationandevolutionofmosaicaneuploidyinleishmaniadonovani AT yagoubatakila highthroughputsinglecellgenomesequencinggivesinsightsintothegenerationandevolutionofmosaicaneuploidyinleishmaniadonovani AT vandenbroeckfrederik highthroughputsinglecellgenomesequencinggivesinsightsintothegenerationandevolutionofmosaicaneuploidyinleishmaniadonovani AT sterkersyvon highthroughputsinglecellgenomesequencinggivesinsightsintothegenerationandevolutionofmosaicaneuploidyinleishmaniadonovani AT dujardinjeanclaude highthroughputsinglecellgenomesequencinggivesinsightsintothegenerationandevolutionofmosaicaneuploidyinleishmaniadonovani AT domagalskamalgorzataa highthroughputsinglecellgenomesequencinggivesinsightsintothegenerationandevolutionofmosaicaneuploidyinleishmaniadonovani |