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Experimental investigation of early assessment of corpora cavernosa fibrosis with two-dimensional shear wave elastography

This study explored the usefulness of two-dimensional shear wave elastography (2D-SWE) in the early assessment of corpora cavernosa fibrosis (CCF). New Zealand male rabbits were randomly assigned to an experimental group or a control group. Recombinant human transforming growth factor beta 1 (TGF-β1...

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Autores principales: Yu, Li, Rao, Wan-Ting, Tang, Jing-Dong, Xing, Jin-Fang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8887103/
https://www.ncbi.nlm.nih.gov/pubmed/34494557
http://dx.doi.org/10.4103/aja202147
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author Yu, Li
Rao, Wan-Ting
Tang, Jing-Dong
Xing, Jin-Fang
author_facet Yu, Li
Rao, Wan-Ting
Tang, Jing-Dong
Xing, Jin-Fang
author_sort Yu, Li
collection PubMed
description This study explored the usefulness of two-dimensional shear wave elastography (2D-SWE) in the early assessment of corpora cavernosa fibrosis (CCF). New Zealand male rabbits were randomly assigned to an experimental group or a control group. Recombinant human transforming growth factor beta 1 (TGF-β1) was injected into the dorsal penis tissue of rabbits in the experimental group. Conventional ultrasound and 2D-SWE examinations were performed before and 20 days after injection. Penile histological analysis was performed by hematoxylin–eosin staining, sirius red staining, and immunohistochemistry. Measurement of 2D-SWE examination results was performed using shear wave elastography quantitative measurement (SWQ). Histological analysis outcomes were the proportion of smooth muscle cells (SMCs), collagen fibers (CFs), collagen type I (Col I), and collagen type III (Col III), as well as the SMCs/CFs ratio, measured by sirius red staining. Other histological analysis outcomes were the positive area proportion (PAP) of TGF-β1 (PAP(T)), fibronectin (PAP(F)), and Col III (PAP(C)), measured by immunohistochemistry. After recombinant human TGF-β1 injection, SWQ was higher in the experimental group than that in the control group (P < 0.001); however, there were no differences in conventional ultrasound results. There were significant differences in histological outcomes between the two groups (all P < 0.05). These results indicated that 2D-SWE was superior for identifying early histological changes in CCF.
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spelling pubmed-88871032022-03-10 Experimental investigation of early assessment of corpora cavernosa fibrosis with two-dimensional shear wave elastography Yu, Li Rao, Wan-Ting Tang, Jing-Dong Xing, Jin-Fang Asian J Androl Original Article This study explored the usefulness of two-dimensional shear wave elastography (2D-SWE) in the early assessment of corpora cavernosa fibrosis (CCF). New Zealand male rabbits were randomly assigned to an experimental group or a control group. Recombinant human transforming growth factor beta 1 (TGF-β1) was injected into the dorsal penis tissue of rabbits in the experimental group. Conventional ultrasound and 2D-SWE examinations were performed before and 20 days after injection. Penile histological analysis was performed by hematoxylin–eosin staining, sirius red staining, and immunohistochemistry. Measurement of 2D-SWE examination results was performed using shear wave elastography quantitative measurement (SWQ). Histological analysis outcomes were the proportion of smooth muscle cells (SMCs), collagen fibers (CFs), collagen type I (Col I), and collagen type III (Col III), as well as the SMCs/CFs ratio, measured by sirius red staining. Other histological analysis outcomes were the positive area proportion (PAP) of TGF-β1 (PAP(T)), fibronectin (PAP(F)), and Col III (PAP(C)), measured by immunohistochemistry. After recombinant human TGF-β1 injection, SWQ was higher in the experimental group than that in the control group (P < 0.001); however, there were no differences in conventional ultrasound results. There were significant differences in histological outcomes between the two groups (all P < 0.05). These results indicated that 2D-SWE was superior for identifying early histological changes in CCF. Wolters Kluwer - Medknow 2021-09-03 /pmc/articles/PMC8887103/ /pubmed/34494557 http://dx.doi.org/10.4103/aja202147 Text en Copyright: ©The Author(s)(2021) https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Yu, Li
Rao, Wan-Ting
Tang, Jing-Dong
Xing, Jin-Fang
Experimental investigation of early assessment of corpora cavernosa fibrosis with two-dimensional shear wave elastography
title Experimental investigation of early assessment of corpora cavernosa fibrosis with two-dimensional shear wave elastography
title_full Experimental investigation of early assessment of corpora cavernosa fibrosis with two-dimensional shear wave elastography
title_fullStr Experimental investigation of early assessment of corpora cavernosa fibrosis with two-dimensional shear wave elastography
title_full_unstemmed Experimental investigation of early assessment of corpora cavernosa fibrosis with two-dimensional shear wave elastography
title_short Experimental investigation of early assessment of corpora cavernosa fibrosis with two-dimensional shear wave elastography
title_sort experimental investigation of early assessment of corpora cavernosa fibrosis with two-dimensional shear wave elastography
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8887103/
https://www.ncbi.nlm.nih.gov/pubmed/34494557
http://dx.doi.org/10.4103/aja202147
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