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Hemostatic Activity of Canine Never-Frozen Liquid Plasma Collected for Transfusion

This study measured the changes of hemostatic activity in liquid plasma (LP) over 7 days of storage. Five canine plasma units, divided into two aliquots were evaluated: one stored refrigerated at 2–6°C as never-frozen LP and one frozen at −18°C as fresh frozen plasma (FFP). Clotting times, coagulati...

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Autores principales: Proverbio, Daniela, Perego, Roberta, Baggiani, Luciana, Spada, Eva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8887598/
https://www.ncbi.nlm.nih.gov/pubmed/35242834
http://dx.doi.org/10.3389/fvets.2022.731617
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author Proverbio, Daniela
Perego, Roberta
Baggiani, Luciana
Spada, Eva
author_facet Proverbio, Daniela
Perego, Roberta
Baggiani, Luciana
Spada, Eva
author_sort Proverbio, Daniela
collection PubMed
description This study measured the changes of hemostatic activity in liquid plasma (LP) over 7 days of storage. Five canine plasma units, divided into two aliquots were evaluated: one stored refrigerated at 2–6°C as never-frozen LP and one frozen at −18°C as fresh frozen plasma (FFP). Clotting times, coagulation activities of factor (F) V, VIII, X, XI, antithrombin (AT), and von Willebrand (vWF), fibrinogen and D-dimers (DD) content were assessed before storage (baseline value), and after 12, 24, 48 h and 7 days (D7) in LP stored refrigerated, and on day 7 in FFP. At baseline median values of all factor activity were greater than 80%, and for clotting times, AT, fibrinogen and DD content, were within the canine reference range. Some hemostatic parameters changed significantly over 7 days and at the end of storage in LP. However, median activities of FV, FVIII, FX and FXI, coagulation time, AT, fibrinogen and DD content remained within reference ranges at all time points. The only exception was for vWF which median activity was lower than reference range for all storage time points. Activity of FVIII was significant lower in LP at D7 when compared to activity in FFP, with values of 62 vs. 118%, respectively. DD content showed a median value higher than reference range in FFP at D7. Despite some statistically significant changes at the end of 7-day storage period, never-frozen LP maintained median factor activities >80% for most factors. The clinical impact of the drop over time of vWF activity is unknown.
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spelling pubmed-88875982022-03-02 Hemostatic Activity of Canine Never-Frozen Liquid Plasma Collected for Transfusion Proverbio, Daniela Perego, Roberta Baggiani, Luciana Spada, Eva Front Vet Sci Veterinary Science This study measured the changes of hemostatic activity in liquid plasma (LP) over 7 days of storage. Five canine plasma units, divided into two aliquots were evaluated: one stored refrigerated at 2–6°C as never-frozen LP and one frozen at −18°C as fresh frozen plasma (FFP). Clotting times, coagulation activities of factor (F) V, VIII, X, XI, antithrombin (AT), and von Willebrand (vWF), fibrinogen and D-dimers (DD) content were assessed before storage (baseline value), and after 12, 24, 48 h and 7 days (D7) in LP stored refrigerated, and on day 7 in FFP. At baseline median values of all factor activity were greater than 80%, and for clotting times, AT, fibrinogen and DD content, were within the canine reference range. Some hemostatic parameters changed significantly over 7 days and at the end of storage in LP. However, median activities of FV, FVIII, FX and FXI, coagulation time, AT, fibrinogen and DD content remained within reference ranges at all time points. The only exception was for vWF which median activity was lower than reference range for all storage time points. Activity of FVIII was significant lower in LP at D7 when compared to activity in FFP, with values of 62 vs. 118%, respectively. DD content showed a median value higher than reference range in FFP at D7. Despite some statistically significant changes at the end of 7-day storage period, never-frozen LP maintained median factor activities >80% for most factors. The clinical impact of the drop over time of vWF activity is unknown. Frontiers Media S.A. 2022-02-15 /pmc/articles/PMC8887598/ /pubmed/35242834 http://dx.doi.org/10.3389/fvets.2022.731617 Text en Copyright © 2022 Proverbio, Perego, Baggiani and Spada. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Proverbio, Daniela
Perego, Roberta
Baggiani, Luciana
Spada, Eva
Hemostatic Activity of Canine Never-Frozen Liquid Plasma Collected for Transfusion
title Hemostatic Activity of Canine Never-Frozen Liquid Plasma Collected for Transfusion
title_full Hemostatic Activity of Canine Never-Frozen Liquid Plasma Collected for Transfusion
title_fullStr Hemostatic Activity of Canine Never-Frozen Liquid Plasma Collected for Transfusion
title_full_unstemmed Hemostatic Activity of Canine Never-Frozen Liquid Plasma Collected for Transfusion
title_short Hemostatic Activity of Canine Never-Frozen Liquid Plasma Collected for Transfusion
title_sort hemostatic activity of canine never-frozen liquid plasma collected for transfusion
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8887598/
https://www.ncbi.nlm.nih.gov/pubmed/35242834
http://dx.doi.org/10.3389/fvets.2022.731617
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