Isolation and freezing of human peripheral blood mononuclear cells from pregnant patients

To analyze immune cell populations accurately, a large number of Peripheral Blood Mononuclear Cells (PBMCs) must be obtained from blood samples. Traditional manual isolation and SepMate(TM) isolation of PBMCs consistently yield blood-stained plasma layers and overall low numbers of CD4+ and CD8+ cel...

Descripción completa

Detalles Bibliográficos
Autores principales: Efthymiou, Athina, Mureanu, Nicoleta, Pemberton, Rebecca, Tai-MacArthur, Sarah, Mastronicola, Daniela, Scottà, Cristiano, Lombardi, Giovanna, Nicolaides, Kypros H., Shangaris, Panicos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8888982/
https://www.ncbi.nlm.nih.gov/pubmed/35252886
http://dx.doi.org/10.1016/j.xpro.2022.101204
Descripción
Sumario:To analyze immune cell populations accurately, a large number of Peripheral Blood Mononuclear Cells (PBMCs) must be obtained from blood samples. Traditional manual isolation and SepMate(TM) isolation of PBMCs consistently yield blood-stained plasma layers and overall low numbers of CD4+ and CD8+ cells. Here, we describe an optimized protocol, using PBS with EDTA to increase PBMC yield from pregnant patients. This protocol enables analysis of CD4+, CD8+, and Regulatory T Cells and is potentially applicable to any immune cell population. For complete details on the use and execution of this protocol, please refer to the SepMateTM website https://www.stemcell.com/products/brands/SepMateTM-pbmc-isolation.html.

Ejemplares similares